Information on EC 3.5.1.67 - 4-methyleneglutaminase

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The expected taxonomic range for this enzyme is: Arachis hypogaea

EC NUMBER
COMMENTARY hide
3.5.1.67
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RECOMMENDED NAME
GeneOntology No.
4-methyleneglutaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-methylene-L-glutamine + H2O = 4-methylene-L-glutamate + NH3
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
C5-Branched dibasic acid metabolism
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SYSTEMATIC NAME
IUBMB Comments
4-methylene-L-glutamine amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
86855-36-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-L-glutamine + H2O
4-methyl-L-glutamate + NH3
show the reaction diagram
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about 9% of the rate of 4-methylene-L-glutamine hydrolysis
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?
4-methylene-L-glutamine + H2O
4-methylene-L-glutamate + NH3
show the reaction diagram
4-methylene-L-glutamine + H2O
?
show the reaction diagram
L-glutamate-5-ethyl ester + H2O
?
show the reaction diagram
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about 1.6% of the rate of 4-methylene-L-glutamine hydrolysis
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?
L-glutamate-5-hydroxamate + H2O
?
show the reaction diagram
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about 6.6% of the rate of 4-methylene-L-glutamine hydrolysis
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?
L-glutamate-5-methyl ester + H2O
?
show the reaction diagram
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about 9% of the rate of 4-methylene-L-glutamine hydrolysis
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?
L-glutamine + H2O
L-glutamate + NH3
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-methylene-L-glutamine + H2O
?
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na2HPO4
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0.15 M, substrate 4-methylene-L-glutamine: very slight activation, substrate L-glutamine: no effect
Na2SO4
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0.15 M, substrate 4-methylene-L-glutamine: very slight activation, substrate L-glutamine: no effect
phosphate
Phosphate esters
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slight activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-methylene-L-glutamate
4-methylene-L-glutamine
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competitive, glutamine as substrate, Ki: 30 mM
albizziine
arsenate
BO33-
D-glutamine
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20 mM: 20% inhibition
diazo-oxo-norleucine
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very slight inhibition, 4 mM: 7% inhibition
I-
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slight inhibition
L-asparagine
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very slight inhibition, 20 mM: 3% inhibition
L-glutamate
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non-competitive, Ki: 5.4 mM
L-Glutamate-5-ethyl ester
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20 mM: 40% inhibition
L-glutamate-5-hydroxamate
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competitive, 20 mM: 35% inhibition, 4-methylene-L-glutamine as substrate
L-glutamate-5-methyl ester
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20 mM: 63% inhibition
L-glutamate-diethyl ester
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20 mM: 40% inhibition
L-glutamine
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competitive, 20 mM: 50% inhibition, 4-methylene-L-glutamine as substrate
L-N5-Ethylglutamine
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theanine, slight inhibition, 20 mM: 14% inhibition
N-acetyl-L-glutamate
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20 mM: 78% inhibition
N-acetyl-L-glutamine
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20 mM: 29% inhibition
NaBr
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slight inhibition
NaCl
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slight inhibition
NaCN
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very slight inhibition
NaI
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slight inhibition
Phenol red
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competitive, Ki: 3.8 mM
S-Carbamoyl-L-cysteine
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20 mM: 78% inhibition
Urea
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slight inhibition, 10 mM: 10% inhibition
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
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slight activation
benzalkonium chloride
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activation, 126% activity
bovine serum albumin
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activation, 0.5%: 180% activity
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Detergents
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ionic or non-ionic detergents, 3-4fold activation
methionine sulfoximine
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very slight activation
rabbit preimmune serum
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activation, 0.5%: 180% activity
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SDS
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0.001-1%, 2-3fold stimulation, 4-methylene-L-glutamine or glutamine as substrate; activation
Sodium deoxycholate
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activation, 160% activity
sodium octanoate
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activation, 130% activity
Triton X-100
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activation, 180% activity
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3 - 67
4-methylene-L-glutamine
20
L-glutamate-5-hydroxamate
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13 - 67
L-glutamine
additional information
additional information
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30
4-methylene-L-glutamine
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competitive, glutamine as substrate
20
albizziine
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5.4
L-glutamate
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non-competitive
3.8
Phenol red
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competitive
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49.3 - 106
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3 multiple forms of 4-methylene-L-glutamine amidohydrolase: AH-1: 49.3 micromol/min/mg, AH-2: 106.0 micromol/min/mg, AH-3: 77.3 micromol/min/mg
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9
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broad pH optimum
8.5 - 11
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broad plateau
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 10.5
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pH 6.5: 47% of maximal activity, pH 10.5: 27% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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27% of total activity
Manually annotated by BRENDA team
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2% of total activity
Manually annotated by BRENDA team
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3% of total activity
Manually annotated by BRENDA team
additional information
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level of 4-methylene-L-glutamine amidohydrolase activity increases rapidly with germination to a maximum after 3 weeks; tissue distribution, not in root, imbibed seeds and mature plants
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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present in soluble fraction
Manually annotated by BRENDA team
additional information
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subcellular localization
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
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gel filtration
48600
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1 * 48600, SDS-PAGE
73000
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sucrose density gradient centrifugation, possibly the result of aggregation in the presence of high sucrose concentrations
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 48600, SDS-PAGE
additional information
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probably a heterodimer, band II: 50000, probably a homodimer, PAGE; two electrophoretically distinct catalytically active forms: band I: 57000 and 50000
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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stable up to, above 40°C: activity lost rapidly, glutamine protects against thermal denaturation
55
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10 min, about 50% loss of activity, glutamine protects against thermal denaturation
additional information
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glutamine protects against thermal denaturation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glutamine stabilizes, protects against thermal denaturation
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in solution, stable for many months
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4°C, stable for at least 4 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from fresh or frozen leaves; multiple electrophoretically distinct forms; partial
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multiple electrophoretically distinct forms; two catalytically active forms
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