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Literature summary for 3.5.1.67 extracted from

  • Ibrahim, S.A.; Lea, P.J.; Fowden, L.
    Purification and properties of 4-methyleneglutaminase from the leaves of peanut (Arachis hypogaea) (1984), Phytochemistry, 23, 1545-1549.
No PubMed abstract available
Search for more literature for 3.5.1.67

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by anions or carboxylic acids Arachis hypogaea

General Stability

General Stability Organism
glutamine stabilizes, protects against thermal denaturation Arachis hypogaea

Inhibitors

Inhibitors Comment Organism Structure
4-methylene-L-glutamate non-competitive, Ki: 5.4 mM Arachis hypogaea
albizziine 20 mM: 50% inhibition Arachis hypogaea
arsenate very slight inhibition Arachis hypogaea
BO33- strong inhibition, non-competitive, Ki: 0.125 mM Arachis hypogaea
Cu2+ 1 mM: 20% inhibition Arachis hypogaea
D-glutamine 20 mM: 20% inhibition Arachis hypogaea
I- slight inhibition Arachis hypogaea
L-asparagine very slight inhibition, 20 mM: 3% inhibition Arachis hypogaea
L-glutamate non-competitive, Ki: 5.4 mM Arachis hypogaea
L-Glutamate-5-ethyl ester 20 mM: 40% inhibition Arachis hypogaea
L-glutamate-5-hydroxamate competitive, 20 mM: 35% inhibition, 4-methylene-L-glutamine as substrate Arachis hypogaea
L-glutamate-5-methyl ester 20 mM: 63% inhibition Arachis hypogaea
L-glutamate-diethyl ester 20 mM: 40% inhibition Arachis hypogaea
L-glutamine competitive, 20 mM: 50% inhibition, 4-methylene-L-glutamine as substrate Arachis hypogaea
L-N5-Ethylglutamine theanine, slight inhibition, 20 mM: 14% inhibition Arachis hypogaea
additional information insensitive to a variety of salts and carboxylic acids; kinetics of inhibition; no inhibition by phosphate, EDTA, a wide range of mono- and divalent cations, pyruvate, 2-oxoglutarate, SO42- Arachis hypogaea
N-acetyl-L-glutamate 20 mM: 78% inhibition Arachis hypogaea
N-acetyl-L-glutamine 20 mM: 29% inhibition Arachis hypogaea
NaBr slight inhibition Arachis hypogaea
NaCl slight inhibition Arachis hypogaea
NaCN very slight inhibition Arachis hypogaea
NaI slight inhibition Arachis hypogaea
Phenol red competitive, Ki: 3.8 mM Arachis hypogaea
S-Carbamoyl-L-cysteine 20 mM: 78% inhibition Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data Arachis hypogaea
additional information
-
 additional information kinetics of inhibition Arachis hypogaea
13
-
 L-glutamine pH 7.2 Arachis hypogaea
13
-
 4-methylene-L-glutamine pH: 7.2 Arachis hypogaea
16
-
 L-glutamine pH 7.6 Arachis hypogaea
16
-
 4-methylene-L-glutamine pH: 7.6 Arachis hypogaea
20
-
 L-glutamate-5-hydroxamate
-
 Arachis hypogaea
20
-
 L-glutamine pH: 8.0 Arachis hypogaea
20
-
 4-methylene-L-glutamine pH: 8.0 Arachis hypogaea
27
-
 L-glutamine pH: 6.8 Arachis hypogaea
29
-
 4-methylene-L-glutamine pH: 6.8 Arachis hypogaea
36
-
 4-methylene-L-glutamine pH: 6.4 Arachis hypogaea
36
-
 L-glutamine pH 6.4 Arachis hypogaea
40
-
 L-glutamine pH 8.4 Arachis hypogaea
50
-
 4-methylene-L-glutamine pH: 8.4 Arachis hypogaea
67
-
 L-glutamine pH 6.0 Arachis hypogaea
67
-
 4-methylene-L-glutamine pH: 6.0 Arachis hypogaea

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions not involved in catalytic reaction Arachis hypogaea
Na2HPO4 0.15 M, substrate 4-methylene-L-glutamine: very slight activation, substrate L-glutamine: no effect Arachis hypogaea
Na2SO4 0.15 M, substrate 4-methylene-L-glutamine: very slight activation, substrate L-glutamine: no effect Arachis hypogaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methylene-L-glutamine + H2O Arachis hypogaea
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
 peanut, groundnut
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arachis hypogaea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arachis hypogaea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.042
-
-
 Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylene-L-glutamine + H2O
-
 Arachis hypogaea 4-methylene-L-glutamate + NH3
-
 ?
4-methylene-L-glutamine + H2O
-
 Arachis hypogaea ?
-
 ?
L-glutamate-5-ethyl ester + H2O about 1.6% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea ?
-
 ?
L-glutamate-5-hydroxamate + H2O about 6.6% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea ?
-
 ?
L-glutamate-5-methyl ester + H2O about 9% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea ?
-
 ?
L-glutamine + H2O about 20% of the rate of 4-methylene-L-glutamine hydrolysis Arachis hypogaea L-glutamate + NH3
-
 ?
additional information substrate specificity Arachis hypogaea ?
-
 ?
additional information hydrolyses compounds with substituents on the amide group, e.g. esters and the hydroxamic acid derivative Arachis hypogaea ?
-
 ?
additional information L-N5-ethylglutamine, theanine, N-methyl-DL-glutamic acid, N-carbobenzoxy-L-glutamic acid, L-glutamic acid diethyl ester, N-acetyl-L-glutamine, D-glutamine, L-asparagine, O-carbamoyl-L-serine, S-carbamoyl-L-cysteine, albizziine, N-acetyl-L-glutamic acid: no substrates Arachis hypogaea ?
-
 ?
additional information L-asparagine: not a substrate Arachis hypogaea ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arachis hypogaea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 glutamine protects against thermal denaturation Arachis hypogaea
40
-
 stable up to, above 40°C: activity lost rapidly, glutamine protects against thermal denaturation Arachis hypogaea
55
-
 10 min, about 50% loss of activity, glutamine protects against thermal denaturation Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 activity sensitive to buffer composition Arachis hypogaea
8.5 11 broad plateau Arachis hypogaea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.8
-
 Phenol red competitive Arachis hypogaea
5.4
-
 L-glutamate non-competitive Arachis hypogaea
20
-
 albizziine
-
 Arachis hypogaea