Information on EC 3.5.1.17 - acyl-lysine deacylase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.1.17
-
RECOMMENDED NAME
GeneOntology No.
acyl-lysine deacylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N6-acyl-L-lysine + H2O = a carboxylate + L-lysine
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lysine degradation
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-
SYSTEMATIC NAME
IUBMB Comments
N6-acyl-L-lysine amidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9025-11-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
EA ATCC 23584
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Manually annotated by BRENDA team
No. 10, mould
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-
Manually annotated by BRENDA team
pigeon
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Manually annotated by BRENDA team
chicken
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-
Manually annotated by BRENDA team
Mushroom
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
pig
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-methionine + H2O
methionine
show the reaction diagram
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-
-
-
?
epsilon-N-acyl-L-lysine + H2O
?
show the reaction diagram
N2-acetyl-L-lysine + H2O
L-lysine + acetate
show the reaction diagram
N2-benzoyl-L-lysine + H2O
L-lysine + benzoate
show the reaction diagram
N4-acetyl-L-diaminobutyric acid + H2O
L-diaminobutyrate + acetate
show the reaction diagram
-
-
-
-
?
N5-nicotinyl-L-ornithine + H2O
L-ornithine + nicotinate
show the reaction diagram
N5-propionyl-L-ornithine + H2O
L-ornithine + propionate
show the reaction diagram
N6-acetyl-L-lysine + H2O
L-lysine + acetate
show the reaction diagram
N6-acetyl-ornithine + H2O
L-ornithine + acetate
show the reaction diagram
N6-acyl-L-lysine + H2O
a carboxylate + L-lysine
show the reaction diagram
the enzyme specifically catalyzes hydrolysis of the epsilon-amide bond in various Nepsilon-acyl-L-lysines
-
-
?
N6-acyl-L-lysine + H2O
L-lysine + carboxylic acid
show the reaction diagram
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-
-
-
?
N6-alpha-naphthoyl-L-lysine + H2O
L-lysine + 1-naphthol
show the reaction diagram
-
-
-
-
?
N6-benzoyl-L-lysine + H2O
L-lysine + benzoate
show the reaction diagram
N6-butyryl-L-lysine + H2O
L-lysine + butanoate
show the reaction diagram
-
-
-
-
?
N6-caproyl-L-lysine + H2O
L-lysine + n-hexanoate
show the reaction diagram
-
-
-
-
?
N6-caprylyl-L-lysine + H2O
L-lysine + caprylic acid
show the reaction diagram
-
-
-
-
?
N6-carbobenzoxy-L-lysine + H2O
L-lysine + benzoate
show the reaction diagram
N6-chloroacetyl-L-lysine + H2O
L-lysine + chloroacetate
show the reaction diagram
N6-chloroacetyl-ornithine + H2O
L-ornithine + chloroacetate
show the reaction diagram
N6-cyclohexanecarboxyl-L-lysine + H2O
L-lysine + cyclohexanecarboxylate
show the reaction diagram
-
-
-
-
?
N6-dichloroacetyl-L-lysine + H2O
L-lysine + dichloroacetate
show the reaction diagram
-
-
-
-
?
N6-formyl-L-lysine + H2O
L-lysine + formate
show the reaction diagram
N6-glycyl-L-lysine + H2O
L-lysine + glycine
show the reaction diagram
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-
-
-
?
N6-iodoacetyl-L-lysine + H2O
L-lysine + iodoacetate
show the reaction diagram
-
-
-
-
?
N6-isobutyryl-L-lysine + H2O
L-lysine + isobutyrate
show the reaction diagram
-
-
-
-
?
N6-m-nitrobenzoyl-L-lysine + H2O
L-lysine + 3-nitrobenzoate
show the reaction diagram
-
-
-
-
?
N6-metacrylyl-L-lysine + H2O
L-lysine + ?
show the reaction diagram
-
-
-
-
?
N6-nicotinyl-L-Lysine + H2O
L-lysine + nicotinate
show the reaction diagram
N6-p-nitrobenzoyl-L-lysine + H2O
L-lysine + 4-nitrobenzoate
show the reaction diagram
-
-
-
-
?
N6-phenylacetyl-L-lysine + H2O
L-lysine + phenylacetate
show the reaction diagram
-
-
-
-
?
N6-propionyl-lysine + H2O
L-lysine + propionate
show the reaction diagram
N6-propionyl-ornithine + H2O
L-ornithine + propionate
show the reaction diagram
N6-trichloroacetyl-L-lysine + H2O
L-lysine + trichloroacetate
show the reaction diagram
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-
-
-
?
zeta-N-acetyl-DL-homolysine + H2O
?
show the reaction diagram
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-
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
epsilon-N-acyl-L-lysine + H2O
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
citrate
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slight inhibitory effect
diphosphate
-
marked inhibition
EDTA
-
slight inhibitory effect
Monoiodoacetic acid
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omega-chloroacetophenone
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oxalate
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marked inhibition
p-chloromercuribenzoate
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inhibition is completely recovered by addition of cysteine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
N6-acetyl-L-lysine
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-
0.1
N6-benzoyl-L-lysine
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-
0.29
N6-chloroacetyl-L-lysine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.087
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-
14.5
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homogenous protein, vertical zone electrophoresis, purification 60.4fold
240
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cell-free extract, purification 1fold
2500 - 2800
purified recombinant enzyme, pH not specified in the publication, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2 - 8.4
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-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55816
x * 55816, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55816, sequence calculation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 60% ammonium sulfate, can be stored several months without decrease of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme to homogeneity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression as soluble secreted enzyme in Streptomyces lividans strain TK24, subcloning in Escherichia coli strain DH5alpha