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Literature summary for 3.5.1.17 extracted from
- Efficient Nepsilon-lauroyl-L-lysine production by recombinant epsilon-lysine acylase from Streptomyces mobaraensis (2009), J. Biotechnol., 141, 160-165.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression as soluble secreted enzyme in Streptomyces lividans strain TK24, subcloning in Escherichia coli strain DH5alpha | Streptomyces mobaraensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55816 | - |
x * 55816, sequence calculation | Streptomyces mobaraensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces mobaraensis | C4B800 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme to homogeneity | Streptomyces mobaraensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2500 | 2800 | purified recombinant enzyme, pH not specified in the publication, temperature not specified in the publication | Streptomyces mobaraensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Sm-ELA catalyzes not only the hydrolysis of various Nepsilon-acyl-L-lysines but also the synthesis of Nepsilon-acyl-L-lysines from L-lysine hydrochloride and medium-/long-chain fatty acids, including Nepsilon-lauroyl-L-lysine | Streptomyces mobaraensis | ? | - |
? |  |
| N6-acyl-L-lysine + H2O | the enzyme specifically catalyzes hydrolysis of the epsilon-amide bond in various Nepsilon-acyl-L-lysines | Streptomyces mobaraensis | a carboxylate + L-lysine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55816, sequence calculation | Streptomyces mobaraensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ELA | - |
Streptomyces mobaraensis |
| epsilon-lysine acylase | - |
Streptomyces mobaraensis |
| More | the enzyme belongs to the YtcJ-like metal-dependent amidohydrolase family, which is further characterized as the metallo-dependent hydrolase superfamily | Streptomyces mobaraensis |
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