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Information on EC 3.4.24.69 - bontoxilysin

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.69 bontoxilysin
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UNIPROT: Q57236 not found.
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates
Synonyms
botulinum toxin, botulinum toxin type a, bont-a, bont/a, onabotulinumtoxina, botulinum neurotoxin, bonta, incobotulinumtoxina, bont/b, abobotulinumtoxina, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
botulinum neurotoxin serotype F
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BoNT
-
-
-
-
BoNT/B
-
-
-
-
BoNT/C1
-
-
-
-
BoNT/D
-
-
-
-
BoNT/E
-
-
-
-
BoNT/F
-
-
-
-
BoNT/G
-
-
-
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Bontoxilysin C1
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Botulinum neurotoxin
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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CAS REGISTRY NUMBER
COMMENTARY hide
107231-12-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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BoNTs bind motor neurons via ganglioside-protein dual receptors, i.e. two HCR/F binding glycans: ganglioside GD1a and oligosaccharides containing an N-acetyllactosamine core, HCR/F binds synaptic vesicle glycoproteins through the keratan sulfate moiety of SV2, structure-function properties of BoNT/F host receptor interactions, dual receptors for BoNT/F, overview. Deglycosylation of glycoproteins disrupts the interaction with HCR/F, while the binding of HCR/B to its cognate receptor, synaptotagmin I, is unaffected. Mutations within the putative ganglioside binding pocket of HCR/F decrease binding to gangliosides, synaptic vesicle protein complexes, and primary rat hippocampal neurons, overview
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
BoNTs bind motor neurons via ganglioside-protein dual receptors, i.e. two HCR/F binding glycans: ganglioside GD1a and oligosaccharides containing an N-acetyllactosamine core, HCR/F binds synaptic vesicle glycoproteins through the keratan sulfate moiety of SV2, structure-function properties of BoNT/F host receptor interactions, dual receptors for BoNT/F, overview. Deglycosylation of glycoproteins disrupts the interaction with HCR/F, while the binding of HCR/B to its cognate receptor, synaptotagmin I, is unaffected. Mutations within the putative ganglioside binding pocket of HCR/F decrease binding to gangliosides, synaptic vesicle protein complexes, and primary rat hippocampal neurons, overview
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-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
receptor binding assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
receptor binding assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q57236_CLOBO
1278
0
147075
TrEMBL
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
receptor binding domains of BoNT/A and BoNT/F
the crystal structure of the BoNT/F receptor-binding domain in complex with the sugar moiety of ganglioside GD1a is reported. GD1a binds in a shallow groove formed by a conserved peptide motif, with additional stabilizing interactions provided by two arginine residues
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H1241K
mutant shows an increased affinity for GD1a and confers the ability to bind ganglioside GM1a
R1111A/H1241K/R1256A
triple mutant binds GD1a 17fold greater than the double-mutant
R1111A/R1256A
triple mutant binds GD1a 17fold greater than the double-mutant
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fu, Z.; Chen, C.; Barbieri, J.T.; Kim, J.J.; Baldwin, M.R.
Glycosylated SV2 and gangliosides as dual receptors for botulinum neurotoxin serotype F
Biochemistry
48
5631-5641
2009
Clostridium botulinum (Q57236)
Manually annotated by BRENDA team
Benson, M.A.; Fu, Z.; Kim, J.J.; Baldwin, M.R.
Unique ganglioside recognition strategies for clostridial neurotoxins
J. Biol. Chem.
286
34015-34022
2011
Clostridium botulinum (Q57236)
Manually annotated by BRENDA team