Information on EC 3.4.24.31 - mycolysin

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
3.4.24.31
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RECOMMENDED NAME
GeneOntology No.
mycolysin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage of bonds with hydrophobic residues in P1'
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
153190-34-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
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?
casein + H2O
?
show the reaction diagram
furylacryloyl-Gly-Leu-NH2 + H2O
?
show the reaction diagram
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-
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Oxidized insulin B-chain + H2O
?
show the reaction diagram
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cleavage at: Phe1-Val2, His5-Leu6, His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Leu17-Val18, Gly23-Phe24, Phe24-Phe25, Phe25-Tyr26
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additional information
?
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specificity overview: synthetic peptides
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
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contains 1 gatom of zinc per mol of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoacetyl-L-Leu-D-Phe
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weak
2-mercaptoacetyl-L-Leu-L-Phe
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2-mercaptoacetyl-L-Phe-L-Leu
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much more sensitive to inhibition than thermolysin
additional information
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not inhibited by PMSF and iodoacetamide
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37100
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Streptomyces naraensis, gel filtration, sedimentation-diffusion method, sedimentation-viscosity method, amino acid composition
60000
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Streptomyces cacaoi, calculation from nucleotide sequence of the cloned gene
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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contains 1.86% of carbohydrate, with complete absence of hexosamine and sialic acid
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by acetone precipitation and Fractogel CM-650 chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Streptomyces lividans
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in Streptomyces lividans using a pIJ702 vector
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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mutations in the hydrophobic region or in the proline at the boundary of the hydrophobic core-cleavage region delay export of the enzyme and reduce total secretion by a factor of 2-10