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Literature summary for 3.4.24.31 extracted from
- Mycolysin (2004), Handbook of Proteolytic Enzymes(Barrett,A. J. ,Rawlings,N. D. ,Woessner,J. F. ,Eds. )Academic Press, 1, 412-413.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Streptomyces lividans | Streptomyces cacaoi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutations in the hydrophobic region or in the proline at the boundary of the hydrophobic core-cleavage region delay export of the enzyme and reduce total secretion by a factor of 2-10 | Streptomyces cacaoi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM inactivates activity almost completely, addition of 1 mM Zn2+, Co2+ or Mn2+ can restore depressed activity to 100, 142 and 115% respectively | Streptomyces cacaoi |  |
| additional information | not inhibited by PMSF and iodoacetamide | Streptomyces cacaoi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces cacaoi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by acetone precipitation and Fractogel CM-650 chromatography | Streptomyces cacaoi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Streptomyces cacaoi | ? | - |
? | |
| casein + H2O | - |
Streptomyces cacaoi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Streptomyces cacaoi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Streptomyces cacaoi |
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Release 2023.1 (January 2023)
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