Information on EC 3.4.22.B61 - cathepsin L5

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The expected taxonomic range for this enzyme is: Fasciola

EC NUMBER
COMMENTARY hide
3.4.22.B61
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
cathepsin L5
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cathepsin L5 does not exhibit substantial catalytic activity against substrates containing proline in the P2 position (thereby distinguished from Fasciola cathepsin L1, L2 and L3)
show the reaction diagram
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-
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Albumin + H2O
?
show the reaction diagram
-
weak substrate
-
-
?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
CatL5 has a clear preference for benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin over tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
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cathepsin l5 hydrolyzes hemoglobin only at pH 5.5
-
-
?
Immunoglobulin + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
weak substrate
-
-
?
tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
type 1 collagen + H2O
?
show the reaction diagram
-
efficient cleavage of type 1 collagen by CatL5 is limited to the beta-and gamma-chains
-
-
?
type 4 collagen + H2O
?
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
preferred substrate
-
-
?
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02481 - 0.1373
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
0.0059 - 0.017
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
0.05392 - 0.0786
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
0.0318 - 0.0472
tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin
0.0195 - 5392
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
0.01272 - 0.03287
Z-Phe-Arg-7-amido-4-methylcoumarin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.05 - 17.89
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
1387 - 2145
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
0.84 - 1.34
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
582 - 2493
tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin
0.19 - 32.9
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
1.73 - 21.52
Z-Phe-Arg-7-amido-4-methylcoumarin
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
80.5 - 721
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
11711
15.5 - 17
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
8882
2.1 - 40
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
2114
136 - 938
Z-Phe-Arg-7-amido-4-methylcoumarin
24223
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
x * 26000, mature enzyme, SDS-PAGE
38000
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x * 38000, proenzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
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38000 Da proenzyme, 26000 Da mature enzyme
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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wild type CatL5 and mutant CatL5 L69W retain more than 90% activity after 4 h incubation at pH 7.5 and about 40% activity after incubation for 24 h
717293
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nickel chelating column chromatography and Mono S column chromatography
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nickel-chelating Sepharose column chromatography and Mono S column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cathepsin L5 is expressed in COS 7 cells, it induces a humoral response when delivered as secretory constructs in mice
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expressed in Saccharomyces cerevisiae
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expressed in yeast
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expression in Saccharomyces cerevisiae
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expression in yeast; expression of pro-form in yeast
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L69C
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the mutation results in low overall activity
L69F
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the mutant shows a 2-3fold increase in the ability to cleave substrates with P2 proline
L69S
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the mutation results in low overall activity
L69W
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the mutation results in an increased preference for substrates containing proline at P2. The mutant shows increased Km values for benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin and tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin compared to the wild type enzyme, but show increased catalytic efficiency for tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
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vaccination of rats with recombinant zymogen and subsequent challenge with Fasciola hepatica metercercariae. Vaccination results in significantly smaller and fewer flukes than in controls. Maximal protection of 83% is seen in the group vaccinated with cathepsin B and cathepsin L5 in combinantion
medicine