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Literature summary for 3.4.22.B61 extracted from

  • Smooker, P.M.; Whisstock, J.C.; Irving, J.A.
    Siyaguna, S.; Spithill, T.W.: Pike, R.N.: A single amino acid substitution affects substrate specificity in cysteine proteinases from Fasciola hepatica (2000), Protein Sci., 9, 2567-2572.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B61

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Fasciola hepatica

Protein Variants

Protein Variants Comment Organism
L69Y substantial increase in the ability to accommodate substrates with a proline residue in the P2 Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0059
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin FhCatL5-L69Y Fasciola hepatica
0.017
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin FhCatL5 Fasciola hepatica
0.0195
-
 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin FhCatL5-L69Y Fasciola hepatica
0.0286
-
 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin FhCatL5 Fasciola hepatica
0.0318
-
 tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin FhCatL5-L69Y Fasciola hepatica
0.0472
-
 tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin FhCatL5 Fasciola hepatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 x * 26000, mature enzyme, SDS-PAGE Fasciola hepatica

Organism

Organism UniProt Comment Textmining
Fasciola hepatica Q9NB30
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification 38000 Da proenzyme, 26000 Da mature enzyme Fasciola hepatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information cathepsin L5 does not exhibit substantial catalytic activity against substrates containing proline in the P2 position Fasciola hepatica ?
-
 ?
tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica tosyl-Gly-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 26000, mature enzyme, SDS-PAGE Fasciola hepatica

Synonyms

Synonyms Comment Organism
cathepsin L5
-
 Fasciola hepatica
FhCatL5
-
 Fasciola hepatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin FhCatL5 Fasciola hepatica
32.9
-
 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin FhCatL5-L69Y Fasciola hepatica
582
-
 tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin FhCatL5 Fasciola hepatica
1387
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin FhCatL5 Fasciola hepatica
2145
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin FhCatL5-L69Y Fasciola hepatica
2493
-
 tosyl-Gly-Phe-Arg-7-amido-4-methylcoumarin FhCatL5-L69Y Fasciola hepatica