Information on EC 3.4.21.B45 - kallikrein 14

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.B45
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
kallikrein 14
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
proteolytic cleavage of polypeptides
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
342900-46-3
-
9001-01-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
both KLK14 and trypsin are able to activate MAP kinase signalling in the human HEK cells as does the PAR-activating peptide, while KLK8 does not
physiological function
additional information
-
enzyme structure homology modelling using crystal structure, PDB 2BDG, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
airway trypsin-like protease precursor + H2O
?
show the reaction diagram
-
residues 6-10
-
-
?
Ala-Thr-Pro-Lys-Ile-Phe-Asn + H2O
Ala-Thr-Pro-Lys + Ile-Phe-Asn
show the reaction diagram
-
19% cleavage after 1 h incubation
-
-
?
collagen alpha1 (XII and XIX) chain precursor + H2O
?
show the reaction diagram
-
residues 1838-1841
-
-
?
collagen alpha3 (IV) chain precursor + H2O
?
show the reaction diagram
-
residues 832-835
-
-
?
Collagen IV + H2O
?
show the reaction diagram
-
-
-
-
?
D-Ile-Pro-Arg-p-nitroanilide + H2O
D-Ile-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
?
D-Val-Leu-Lys-thiobenzyl ester + H2O
D-Val-Leu-Lys + thiobenzyl alcohol
show the reaction diagram
-
low activity
-
-
?
endothelin-2 precursor + H2O
?
show the reaction diagram
-
residues 145-149
-
-
?
GDDSTPSILPAPRGYPGQV + H2O
GDDSTPSILPAPR + GYPGQV
show the reaction diagram
-
the tethered ligand is GYPGQV
-
-
?
GPNSKGRSLIGRLDTPYGGC + H2O
SLIGRLDTPYGGC + RLDTPYGGC + GPNSKGRSLIG
show the reaction diagram
-
the tethered ligand is SLIGRL
-
-
?
GSLR-4-nitroanilide + H2O
GSLR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
GTNRSSKGRSLIGKVDGTSHVTGKGVT + H2O
GTNRSSKGR + SLIGKVDGTSHVTGKGVT
show the reaction diagram
-
the tethered ligand is SLIGKV
-
-
?
HANR-4-nitroanilide + H2O
HANR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HASR-4-nitroanilide + H2O
HASR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HAVR-4-nitroanilide + H2O
HAVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HLNR-4-nitroanilide + H2O
HLNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HLSR-4-nitroanilide + H2O
HLSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HLVR-4-nitroanilide + H2O
HLVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
human proteinase-activated receptor 2 zymogen + H2O
mature human proteinase-activated receptor 2 + ?
show the reaction diagram
-
unmasking the PAR2 receptor-activating sequence from a peptide precursor
-
-
?
HVNR-4-nitroanilide + H2O
HVNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HVSR-4-nitroanilide + H2O
HVSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
HVVR-4-nitroanilide + H2O
HVVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
L-Gln-Gly-Asp-Lys-Ile-Ile-Asp + H2O
L-Gln-Gly-Asp-Lys + Ile-Ile-Asp
show the reaction diagram
-
41% cleavage after 1 h incubation
-
-
?
L-Glu-Thr-Arg-Ile-Ile-Lys + H2O
L-Glu-Thr-Arg + Ile-Ile-Lys
show the reaction diagram
-
55% cleavage after 1 h incubation
-
-
?
L-Ile-Gln-Ser-Arg-Ile-Val-Gly + H2O
L-Ile-Gln-Ser-Arg + Ile-Val-Gly
show the reaction diagram
-
97% cleavage after 1 h incubation
-
-
?
L-Ile-Leu-Ser-Arg-Ile-Val-Gly + H2O
L-Ile-Leu-Ser-Arg + Ile-Val-Gly
show the reaction diagram
-
87% cleavage after 1 h incubation
-
-
?
L-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
L-Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-Pro-Phe-Arg-p-nitroanilide + H2O
L-Pro-Phe-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
L-Ser-Ser-Ser-Arg-Ile-Ile-Asn + H2O
L-Ser-Ser-Ser-Arg + Ile-Ile-Asn
show the reaction diagram
-
57% cleavage after 1 h incubation
-
-
?
laminin alpha-1 precursor + H2O
?
show the reaction diagram
-
residues 1988-1991
-
-
?
laminin alpha-5 + H2O
?
show the reaction diagram
-
-
-
-
?
MANR-4-nitroanilide + H2O
MANR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MASR-4-nitroanilide + H2O
MASR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
matrillin-4 precursor + H2O
?
show the reaction diagram
-
residues 178-182
-
-
?
MAVR-4-nitroanilide + H2O
MAVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
methoxy-succinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
methoxy-succinyl-Arg-Pro-Tyr + p-nitroaniline
show the reaction diagram
very low activity
-
-
?
MLNR-4-nitroanilide + H2O
MLNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MLSR-4-nitroanilide + H2O
MLSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MLVR-4-nitroanilide + H2O
MLVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MVNR-4-nitroanilide + H2O
MVNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MVSR-4-nitroanilide + H2O
MVSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MVVR-4-nitroanilide + H2O
MVVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
NATLDPRSFLLRNPNDKYE + H2O
NATLDPR + SFLLRNPNDKYE
show the reaction diagram
-
the tethered ligand is SFLLRN
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
-
-
-
?
pro-KLK11 + H2O
KLK11 + ?
show the reaction diagram
-
-
-
-
?
pro-KLK3 + H2O
KLK3 + ?
show the reaction diagram
-
KLK3 is activated by cleavage after arginine at position 7
-
-
?
proprotein convertase subtilisin/kexin type 5 precursor + H2O
?
show the reaction diagram
-
residues 371-375
-
-
?
proteinase-activated receptor 2 zymogen + H2O
mature proteinase-activated receptor 2 + ?
show the reaction diagram
-
-
-
-
?
rat proteinase-activated receptor 2 zymogen + H2O
mature rat proteinase-activated receptor 2 + ?
show the reaction diagram
-
unmasking the PAR2 receptor-activating sequence from a peptide precursor
-
-
?
semenogelin I + H2O
?
show the reaction diagram
-
-
-
-
?
semenogelin II + H2O
?
show the reaction diagram
-
-
-
-
?
serin protease inhibitor Kazal-type 5 precursor + H2O
?
show the reaction diagram
-
residues 1034-1037
-
-
?
tosyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
tosyl-Gly-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
vascular endothelial growth factor receptor 3 precursor + H2O
?
show the reaction diagram
-
residues 1126-1130
-
-
?
VGSLR-4-nitroanilide + H2O
VGSLR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
villin-like protein + H2O
?
show the reaction diagram
-
residues 61-65
-
-
?
WANR-4-nitroanilide + H2O
WANR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WASR-4-nitroanilide + H2O
WASR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WAVR-4-nitroanilide + H2O
WAVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WLNR-4-nitroanilide + H2O
WLNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WLSR-4-nitroanilide + H2O
WLSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WLVR-4-nitroanilide + H2O
WLVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WVNR-4-nitroanilide + H2O
WVNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WVSR-4-nitroanilide + H2O
WVSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
WVVR-4-nitroanilide + H2O
WVVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YAAR-4-nitroanilide + H2O
YAAR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YANR-4-nitroanilide + H2O
YANR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YASR-4-nitroanilide + H2O
YASR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YAVR-4-nitroanilide + H2O
YAVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YLNR-4-nitroanilide + H2O
YLNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YLSR-4-nitroanilide + H2O
YLSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YLVR-4-nitroanilide + H2O
YLVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YVNR-4-nitroanilide + H2O
YVNR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YVSR-4-nitroanilide + H2O
YVSR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
YVVR-4-nitroanilide + H2O
YVVR + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
airway trypsin-like protease precursor + H2O
?
show the reaction diagram
-
residues 6-10
-
-
?
collagen alpha1 (XII and XIX) chain precursor + H2O
?
show the reaction diagram
-
residues 1838-1841
-
-
?
collagen alpha3 (IV) chain precursor + H2O
?
show the reaction diagram
-
residues 832-835
-
-
?
Collagen IV + H2O
?
show the reaction diagram
-
-
-
-
?
endothelin-2 precursor + H2O
?
show the reaction diagram
-
residues 145-149
-
-
?
laminin alpha-1 precursor + H2O
?
show the reaction diagram
-
residues 1988-1991
-
-
?
laminin alpha-5 + H2O
?
show the reaction diagram
-
-
-
-
?
matrillin-4 precursor + H2O
?
show the reaction diagram
-
residues 178-182
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
-
-
-
?
proprotein convertase subtilisin/kexin type 5 precursor + H2O
?
show the reaction diagram
-
residues 371-375
-
-
?
proteinase-activated receptor 2 zymogen + H2O
mature proteinase-activated receptor 2 + ?
show the reaction diagram
-
-
-
-
?
serin protease inhibitor Kazal-type 5 precursor + H2O
?
show the reaction diagram
-
residues 1034-1037
-
-
?
vascular endothelial growth factor receptor 3 precursor + H2O
?
show the reaction diagram
-
residues 1126-1130
-
-
?
villin-like protein + H2O
?
show the reaction diagram
-
residues 61-65
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTG9
-
synthetic inhibitor
-
alpha1-Antichymotrypsin
-
forms covalent complex with hK14
-
Alpha1-antitrypsin
-
forms covalent complex with hK14
-
lympho-epithelial Kazal type inhibitor fragment D5
-
-
-
lympho-epithelial Kazal type inhibitor fragment D8-D11
-
-
-
lympho-epithelial Kazal type inhibitor fragment D9-D15
-
-
-
Spink6
-
i.e. serine protease inhibitor Kazal type 6. Selective inhibitor of kallikreins in the skin. Spink6 possesses only one typical Kazal domain. Its mRNA is expressed at low levels in several tissues and is induced during keratinocyte differentiation. Spink6 is expressed in the stratum granulosum of human skin at various anatomical localisations and in the skin appendages, including sebaceous glands and sweat glands. Spink6 expression is decreased in lesions of atopic dermatitis. Recombinant SPINK6 is inhibitory for KLK5, KLK7 and KLK14
-
Zn2+
-
semenogelins are able to reverse KLK14 inhibition by Zn2+
additional information
-
not inhibited by lympho-epithelial Kazal type inhibitor fragments D1 and D6
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.431
GSLR-4-nitroanilide
-
pH 8.0, 37°C
0.75
L-Pro-Phe-Arg-p-nitroanilide
-
in 0.1 M Tris, 0.5 M NaCl, pH 8
1.675
tosyl-Gly-L-Pro-L-Arg-4-nitroanilide
-
pH 8.0, temperature not specified in the publication
0.318
VGSLR-4-nitroanilide
-
pH 8.0, 37°C
0.034
WASR-4-nitroanilide
-
pH 8.0, 37°C
0.052
WAVR-4-nitroanilide
-
pH 8.0, 37°C
0.0305
WVSR-4-nitroanilide
-
pH 8.0, 37°C
0.043
YAAR-4-nitroanilide
-
pH 8.0, 37°C
0.017
YANR-4-nitroanilide
-
pH 8.0, 37°C
0.022
YASR-4-nitroanilide
-
pH 8.0, 37°C
0.045
YAVR-4-nitroanilide
-
pH 8.0, 37°C
0.025
YVSR-4-nitroanilide
-
pH 8.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.8
GSLR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
44.03
VGSLR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
63.24
WASR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
97.31
WAVR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
42.91
WVSR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
69.37
YAAR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
53.04
YANR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
83.85
YASR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
93.33
YAVR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
34.57
YVSR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
36.6
GSLR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202446
138.3
VGSLR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202445
1880
WASR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202439
1888
WAVR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202440
1408
WVSR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202442
1609
YAAR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202444
3161
YANR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202441
3806
YASR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202437
2074
YAVR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202438
1370
YVSR-4-nitroanilide
Homo sapiens
-
pH 8.0, 37°C
202443
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000176
lympho-epithelial Kazal type inhibitor fragment D5
-
-
-
0.0000031
lympho-epithelial Kazal type inhibitor fragment D8-11
-
-
-
0.0000523
lympho-epithelial Kazal type inhibitor fragment D9-15
-
-
-
0.0000005
Spink6
-
pH 8.0, temperature not specified in the publication
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
KLK14 expression significantly higher compared to normal breast tissues
Manually annotated by BRENDA team
-
of normal bronchus
Manually annotated by BRENDA team
-
benign and malignant glandular epithelium
Manually annotated by BRENDA team
-
KLK13 and KLK14 cannot be considered as specific markers for non-small-cell lung cancer
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
casein zymography
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
pro-kallikrein 14 iss activated with thermolysin
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cation exchange chromatography
-
Superdex gel filtration and reversed-phase liquid chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis
-
expression in Pichia pastoris
-
gene KLK14, recombinant expression in Spodoptera frugiperda Sf9 insect cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in addition to the classical form of the gene, five splice variants were identified. The splicing events involve 5'-truncation or complete elimination of exon 4 and/or retention of intron I. All encoded protein products of the splice variants are predicted to be truncated and catalytically inactive
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I155N
natural polymorphism
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine