Information on EC 3.4.15.4 - Peptidyl-dipeptidase B

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The expected taxonomic range for this enzyme is: Bos taurus

EC NUMBER
COMMENTARY hide
3.4.15.4
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RECOMMENDED NAME
GeneOntology No.
Peptidyl-dipeptidase B
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of a C-terminal dipeptide or exceptionally a tripeptide
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
147014-93-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-Gly-Ala-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Ala + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
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-
-
-
-
2-aminobenzoyl-Gly-Asn-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Asn + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
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-
-
-
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2-aminobenzoyl-Gly-Asp-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Asp + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
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-
-
-
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2-aminobenzoyl-Gly-Lys-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Lys + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
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-
-
-
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2-aminobenzoyl-Gly-Ser-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Ser + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
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-
-
-
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2-aminobenzoyl-Gly-Thr-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Thr + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
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-
-
-
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Atriopeptin II + H2O
Atriopeptin I + ?
show the reaction diagram
Atriopeptin II peptide Ser103-Arg125 + H2O
Atriopeptin I peptide Ser103-Ser123 + Phe-Arg
show the reaction diagram
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-
-
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Atriopeptin III peptide Ser103-Tyr126 + H2O
Atriopeptin I + Phe-Arg-Tyr
show the reaction diagram
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-
-
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Benzoyl-Asn-Ser-Phe-Arg-Tyr + H2O
Benzoyl-Asn-Ser-Phe + Arg-Tyr
show the reaction diagram
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successive dipeptidyl cleavage after longer incubation yielding benzoyl-Asn + Ser-Phe
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Benzoyl-Gly-Asn-Ser + H2O
?
show the reaction diagram
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-
-
-
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benzoyl-Gly-His-Leu + H2O
?
show the reaction diagram
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-
-
-
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Benzoyl-Gly-Phe-Ser-Arg + H2O
?
show the reaction diagram
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-
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[Mercaptopropionyl105,(D)Ala107]-atriopeptinIII-NH2 peptide + H2O
Analog(1-18) + Phe-Arg-Tyr-NH2
show the reaction diagram
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enzyme acts exclusively as tripeptidyl carboxyhydrolase
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additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
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metallopeptidase
additional information
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in contrast to peptidyl-dipeptidase A (EC 3.4.15.1) it displays no dependence on Cl-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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2-D-Methylsuccinyl-L-Pro
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3-Mercaptopropanoyl-L-Pro
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Benzoyl-Asn-Ser-Phe-Arg-Tyr-NH2
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competitive inhibitor of 2-aminobenzoyl-Gly-Ala-Phe(4-nitro)-Arg hydrolysis and benzoyl-Asn-Ser-Phe-Arg-Tyr hydrolysis
Bradykinin potentiating factor
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-
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D-2-Methyl-3-mercaptopropanoyl-L-Pro
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i.e. captopril
DTT
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mercaptoethanol
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additional information
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marked substrate inhibition with benzoyl-Gly-His-Leu, but little or no substrate inhibition with benzoyl-Gly-His-Gly; the enzyme is 5500 times less sensitive to D-Cys-L-Pro, the very potent inhibitor of angiotensin I-converting enzyme (EC 3.4.15.1)
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.102
2-Aminobenzoyl-Gly-Ala-(4-nitro)Phe-Arg
-
-
0.09 - 0.213
2-Aminobenzoyl-Gly-Asp-(4-nitro)Phe-Arg
0.129
2-Aminobenzoyl-Gly-Lys-(4-nitro)Phe-Arg
-
-
0.14
2-Aminobenzoyl-Gly-Ser-(4-nitro)Phe-Arg
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-
0.1
2-Aminobenzoyl-Gly-Thr-(4-nitro)Phe-Arg
0.124 - 0.27
Atriopeptin I
0.025
Atriopeptin II
-
-
1.13
benzoyl-Gly-Gly-Phe-Arg
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-
2.4
benzoyl-Gly-His-Gly
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-
0.8
benzoyl-Gly-His-Leu
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-
0.12
benzoyl-Gly-Ser-Phe-Arg
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
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6.5: about 65% of activity maximum, 9: about 60% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000
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bovine, sucrose density gradient sedimentation, gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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