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Information on EC 3.2.1.81 - beta-agarase

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IUBMB Comments
Also acts on porphyran, but more slowly . This enzyme cleaves the beta-(1->4) linkages of agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place . The end products of hydrolysis are neoagarotetraose and neoagarohexaose in the case of AgaA from the marine bacterium Zobellia galactanivorans, and neoagarotetraose and neoagarobiose in the case of AgaB .
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This record set is specific for:
UNIPROT: Q6F4N4
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
agarase, beta-agarase, aga50d, endo-type beta-agarase, agaac, lsl-1, beta-agarase a, agarase 0107, beta-agarase c, agab34, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agarase
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Agarase 0107
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
agarose 4-glycanohydrolase
Also acts on porphyran, but more slowly [1]. This enzyme cleaves the beta-(1->4) linkages of agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place [6]. The end products of hydrolysis are neoagarotetraose and neoagarohexaose in the case of AgaA from the marine bacterium Zobellia galactanivorans, and neoagarotetraose and neoagarobiose in the case of AgaB [6].
CAS REGISTRY NUMBER
COMMENTARY hide
37288-57-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
agarose + H2O
neoagarotetraose
show the reaction diagram
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-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q6F4N4_9GAMM
433
0
48204
TrEMBL
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
recombinant enzyme
49000
recombinant enzyme
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Bacillus subtilis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fu, X.T.; Kim, S.M.
Agarase: review of major sources, categories, purification method, enzyme characteristics and applications
Mar. drugs
8
200-218
2010
Agarivorans albus (A8W969), Agarivorans albus YKW-34 (A8W969), Agarivorans sp., Agarivorans sp. (A1E2A6), Agarivorans sp. (C9WIW7), Agarivorans sp. JA-1 (A1E2A6), Alteromonas sp., Alteromonas sp. C-1, Alteromonas sp. SY37-12, Bacillus sp. MK03, Cytophaga sp., Flavobacterium flevense, Microbulbifer thermotolerans (Q6F4N4), Microbulbifer thermotolerans JAMB-A94 (Q6F4N4), Pseudoalteromonas antarctica, Pseudoalteromonas antarctica N-1, Pseudomonas sp., Pseudomonas sp. (Q9KK55), Pseudomonas sp. SK38, Pseudomonas sp. W7 (Q9KK55), Saccharophagus degradans (Q21HB4), Saccharophagus degradans (Q21HC5), Saccharophagus degradans (Q21LJ2), Saccharophagus degradans 2-40 (Q21HB9), Saccharophagus degradans 2-40 (Q21LJ1), Saccharophagus degradans 2-40 / ATCC 43961 (Q21HB4), Saccharophagus degradans 2-40 / ATCC 43961 (Q21HC5), Saccharophagus degradans 2-40 / ATCC 43961 (Q21LJ2), Vibrio sp., Vibrio sp. (A1E2B6), Vibrio sp. JT0107, Vibrio sp. PO-303 (A5A6R7), Vibrio sp. V134 (A1E2B6), Zobellia galactanivorans (G0L322), Zobellia galactanivorans (Q9RGX8)
Manually annotated by BRENDA team