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Information on EC 3.2.1.37 - xylan 1,4-beta-xylosidase and Organism(s) Aspergillus niger and UniProt Accession O00089

for references in articles please use BRENDA:EC3.2.1.37
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IUBMB Comments
Also hydrolyses xylobiose. Some other exoglycosidase activities have been found associated with this enzyme in sheep liver.
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This record set is specific for:
Aspergillus niger
UNIPROT: O00089
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The taxonomic range for the selected organisms is: Aspergillus niger
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-xylosidase, alpha-l-arabinofuranosidase, xylosidase, beta-d-xylosidase, xyl i, xyl3a, xynb2, xln-dt, xynb3, axh-d3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,4-beta-D-xylan xylohydrolase
-
-
-
-
beta-D-xylopyranosidase
-
-
-
-
beta-xylosidase
-
-
-
-
beta-xylosidase I
-
soluble form
beta-xylosidase II
-
immobilized on silica or alumina
exo-1,4-beta-D-xylosidase
-
-
-
-
exo-1,4-beta-xylosidase
-
-
-
-
Exo-beta-(1,4)-xylanase
-
-
-
-
glycoside hydrolase family 3 beta-xylosidase
-
Xylan 1,4-beta-xylosidase
-
-
-
-
xylobiase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-xylan xylohydrolase
Also hydrolyses xylobiose. Some other exoglycosidase activities have been found associated with this enzyme in sheep liver.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-53-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl-beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
-
-
?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
show the reaction diagram
-
low activity
-
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
sophorose, low activity
-
?
3 xylotriose
xylotetraose + xylopentaose
show the reaction diagram
-
transxylosylation activity
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
3,4-dinitrophenol + D-xylopyranose
show the reaction diagram
-
-
-
?
3,4-dinitrophenyl beta-D-xyloside + H2O
3,4-dinitrophenol + D-xylose
show the reaction diagram
48 activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
laminaribiose, low activity
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
show the reaction diagram
4-nitrophenyl alpha-L-arabinopyranoside + H2O
4-nitrophenol + L-arabinopyranose
show the reaction diagram
4.9% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
-
low activity
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
0.7% activity compared to 4-nitrophenyl beta-D-xyloside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-xyloside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
100% activity
-
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
cellobiose, 30% activity
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose, low activity
-
?
beechwood xylan + H2O
?
show the reaction diagram
beechwood xylan + H2O
xylobiose + ?
show the reaction diagram
-
-
-
?
birchwood xylan + H2O
beta-D-xylose + ?
show the reaction diagram
-
-
-
-
?
methyl-beta-D-xyloside + H2O
beta-D-xylose + methanol
show the reaction diagram
-
very low activity
-
?
o-nitrophenyl beta-D-xylopyranoside + H2O
o-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-L-arabinoside + H2O
p-nitrophenol + alpha-L-arabinose
show the reaction diagram
-
low activity
-
?
p-nitrophenyl beta-D-xylopyranoside + H2O
p-nitrophenol + beta-D-xylopyranose
show the reaction diagram
phenyl 4-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O
phenyl beta-D-glucopyranoside + D-glucose
show the reaction diagram
-
25% activity
-
?
phenyl beta-D-glucopyranoside + H2O
phenol + D-glucopyranose
show the reaction diagram
-
low activity
-
?
phenyl beta-D-xylopyranoside + H2O
phenol + beta-D-xylopyranose
show the reaction diagram
-
-
-
?
wheat arabinoxylan + H2O
D-xylose + ?
show the reaction diagram
-
-
-
-
?
xylo-oligosaccharides + H2O
beta-D-xylose + ?
show the reaction diagram
-
decrease of activity with increasing polymerization of substrates
-
?
xylobiose + H2O
2 D-xylose
show the reaction diagram
xylobiose + H2O
beta-D-xylose + D-xylose
show the reaction diagram
xylobiose + H2O
xylotriose + ?
show the reaction diagram
-
transxylosylation activity
-
?
xylobiosylxylitol + H2O
xylose + xylitol
show the reaction diagram
-
-
-
?
xylopentaose + H2O
beta-D-xylose + ?
show the reaction diagram
xylosylxylitol + H2O
xylose + xylitol
show the reaction diagram
-
-
-
?
xylotetraose + H2O
xylotriose + beta-D-xylose
show the reaction diagram
xylotriose + H2O
beta-D-xylose + xylobiose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
xylo-oligosaccharides + H2O
beta-D-xylose + ?
show the reaction diagram
-
decrease of activity with increasing polymerization of substrates
-
?
xylobiose + H2O
beta-D-xylose + D-xylose
show the reaction diagram
xylobiose + H2O
xylotriose + ?
show the reaction diagram
-
transxylosylation activity
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
addition of 10 mM CaCl2 enhances the beta-xylosidase activity by 10.22%
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-D-xylose
-
arabinose
-
full activity is observed in the presence of up to 50 mM arabinose. Activity is reduced to 88%, 80% and 70% of maximum activity at 100 mM, 150 mM and 200 mM arabinose, respectively
beta-D-xylose
-
competitive inhibition
CoCl2
-
10 mM, slight inhibition
CuSO4
-
10 mM, 71.34% inhibition
D-xylose
-
end-product inhibition
MgSO4
-
10 mM, slight inhibition
MnSO4
-
10 mM, slight inhibition
p-chloromercuribenzoate
-
-
SDS
-
10 mM, 45% loss of activity
Sodium azide
-
10 mM, 5-6% inhibition
ZnSO4
-
10 mM, 50% loss of activity
additional information
-
upon inclusion of glucose (10-200 mM) in the beta-xylosidase assay reaction mixture, the purified enzyme exhibits full activity relative to the control reaction without added glucose
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.39
3,4-dinitrophenyl beta-D-xylopyranoside
pH 4.0, 40°C
0.39
3,4-dinitrophenyl beta-D-xyloside
at pH 4.0 and 70°C
4.54
4-nitrophenyl alpha-L-arabinofuranoside
pH 4.0, 40°C
4.54
4-nitrophenyl alpha-L-arabinopyranoside
at pH 4.0 and 70°C
4.01
4-nitrophenyl beta-D-glucopyranoside
pH 4.0, 40°C
4.01
4-nitrophenyl beta-D-glucoside
at pH 4.0 and 70°C
0.66 - 1.17
4-nitrophenyl beta-D-xylopyranoside
0.66
4-nitrophenyl beta-D-xyloside
at pH 4.0 and 70°C
0.2 - 0.26
o-nitrophenyl beta-D-xylopyranoside
0.23
p-nitrophenyl beta-D-xylopyranoside
-
-
0.67 - 30.8
xylobiose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
93.8
3,4-dinitrophenyl beta-D-xylopyranoside
pH 4.0, 40°C
93.8
3,4-dinitrophenyl beta-D-xyloside
at pH 4.0 and 70°C
113
4-nitrophenyl alpha-L-arabinofuranoside
pH 4.0, 40°C
113
4-nitrophenyl alpha-L-arabinopyranoside
at pH 4.0 and 70°C
13.5
4-nitrophenyl beta-D-glucopyranoside
pH 4.0, 40°C
13.5
4-nitrophenyl beta-D-glucoside
at pH 4.0 and 70°C
36.08 - 334
4-nitrophenyl beta-D-xylopyranoside
334
4-nitrophenyl beta-D-xyloside
at pH 4.0 and 70°C
67.8
xylobiose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
242
3,4-dinitrophenyl beta-D-xylopyranoside
pH 4.0, 40°C
242
3,4-dinitrophenyl beta-D-xyloside
at pH 4.0 and 70°C
24.9
4-nitrophenyl alpha-L-arabinofuranoside
pH 4.0, 40°C
24.9
4-nitrophenyl alpha-L-arabinopyranoside
at pH 4.0 and 70°C
3.4
4-nitrophenyl beta-D-glucopyranoside
pH 4.0, 40°C
3.37
4-nitrophenyl beta-D-glucoside
at pH 4.0 and 70°C
30.84 - 504
4-nitrophenyl beta-D-xylopyranoside
504
4-nitrophenyl beta-D-xyloside
at pH 4.0 and 70°C
2.2
xylobiose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.33
beta-D-xylose
pH 5.0, 50°C
6.41
xylobiose
pH 5.0, 50°C
7.458
D-xylose
-
70°C, pH 5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.07
-
pH 5.0, 40°C
125 - 140
after 1.68fold purification, at pH 4.0 and 70°C
15.5
-
pH 5.0, 75°C
3.09
-
substrate: 4-nitrophenyl alpha-L-arabinofuranoside, pH 4.0, 65°C
31.5
-
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 4.0, 65°C
5.4
-
substrate: birchwood xylan, pH 4.0, 65°C
61.17
-
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 4.0, 65°C
74.4 - 76.5
crude enzyme, at pH 4.0 and 70°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
both, soluble and immobilized forms
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.4 - 5.5
-
over 69% of maximum activity is observed in the pH range 3.4 to 5.5
3.5 - 4.5
-
pH 3.5: 73% of maximal activity, pH 4.5: 78% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 70
-
60°C: 81% of maximal activity, 70°C: 78% of maximal activity
60 - 77
-
over 50% of maximum activity is observed from 60°C to 77°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
coexpression of enzyme with Trichoderma reesei xylanayse II in Saccharomyces cerevisiae
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
screening of thermophilic and thermotolerant filamentous fungi for the production of beta-xylosidase and arabinanase at different temperatures. beta-Xylosidase and arabinanase activities from mycelial extract (intracellular form) are higher in cultures grown at high temperatures (35-40°C), while the correspondent extracellular activities are favorably secreted from cultures at 30°C. Aspergillus niger and Aspergillus thermomutatus are the filamentous fungi with the most expressive production of beta-xylosidase and arabinanase, respectively
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
XYND_ASPNG
804
0
87211
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85100
x * 85100, calculated from gene sequence
120000
-
SDS-PAGE
120480
-
SDS-PAGE
122000
-
2 * 122000, SDS-PAGE
253000
-
gel filtration, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 85100, calculated from gene sequence
dimer
-
2 * 122000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 6.5
the recombinant enzyme is stable up to pH 6.5, when incubated at 4°C for 4 h
752093
3 - 8
-
stable within
136307
4 - 7
-
stable at 30°C for 24 h, at 70°C for 15 min
136315
5
-
3 h, activity is reduced to 30.21%
750780
6
-
3 h, activity is reduced to 23.07%
750780
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
optimum stability at
30 - 60
the recombinant enzyme shows thermostability up to 60°C with more than 60% activity remaining after incubation at this temperature for 30 min
50
-
stable up to 1 h
70
-
18 h, 48% loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
further purification from commercially available enzyme by 2step chromatogarphy
-
phenyl Sepharose column chromatography
recombinant protein
to homogeneity by 3step chromatography
-
to homogeneity by 4step chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris strains Y11430 and KM71H as an N-terminal fusion protein with the alpha-mating factor signal sequence and a poly-histidine tag
expression in Pichia pastoris
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
coexpression of enzyme with Trichoderma reesei xylanase II in Saccharomyces cerevisiae allows for degradation of birchwood xylan to D-xylose
biofuel production
-
the relatively broad pH profile is favourable for industrial application as it offers potential flexibility in terms of process pH and is in line with the current pH range of lignocellulose enzymatic hydrolysis processes for bioethanol production
degradation
the enzyme is useful for degradation of lignocellulosic biomass in bioethanol production, pulp bleaching process and beverage industry
industry
-
the relatively broad pH profile is favourable for industrial application as it offers potential flexibility in terms of process pH and is in line with the current pH range of lignocellulose enzymatic hydrolysis processes for bioethanol production
synthesis
upon codon optimization for expression in Pichia pastoris, the expression level increases to 5.7 g/l in a fermenter system
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rodionova, N.A.; Tavobilov, I.M.; Bezborodov, A.M.
beta-xylosidase from Aspergillus niger 15: Purification and properties
J. Appl. Biochem.
5
300-312
1983
Aspergillus niger
Manually annotated by BRENDA team
Oguntimein, G.A.; Reilly, P.J.
Purification and immobilization of Aspergillus niger beta-xylosidase
Biotechnol. Bioeng.
22
1127-1142
1980
Aspergillus niger
-
Manually annotated by BRENDA team
Oguntimein, G.A.; Reilly, P.J.
Properties of soluble and immobilized Aspergillus niger beta-xylosidase
Biotechnol. Bioeng.
22
1143-1154
1980
Aspergillus niger
-
Manually annotated by BRENDA team
Takenishi, S.; Tsujisaka, Y.; Fukumoto, J.
Studies on hemicellulases IV. Purification and properties of the beta-xylosidase produced by Aspergillus niger van Tieghem
J. Biochem.
73
335-343
1973
Aspergillus niger
Manually annotated by BRENDA team
La Grange, D.C.; Pretorius, I.S.; Claeyssens, M.; van Zyl, W.H.
Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes
Appl. Environ. Microbiol.
67
5512-5519
2001
Aspergillus niger (O00089), Aspergillus niger
Manually annotated by BRENDA team
Pedersen, M.; Lauritzen, H.K.; Frisvad, J.C.; Meyer, A.S.
Identification of thermostable beta-xylosidase activities produced by Aspergillus brasiliensis and Aspergillus niger
Biotechnol. Lett.
29
743-748
2007
Aspergillus japonicus, Aspergillus niger, Aspergillus brasiliensis
Manually annotated by BRENDA team
Choengpanya, K.; Arthornthurasuk, S.; Wattana-amorn, P.; Huang, W.T.; Plengmuankhae, W.; Li, Y.K.; Kongsaeree, P.T.
Cloning, expression and characterization of beta-xylosidase from Aspergillus niger ASKU28
Protein Expr. Purif.
115
132-140
2015
Aspergillus niger (A0A023J5W7), Aspergillus niger ASKU28 (A0A023J5W7)
Manually annotated by BRENDA team
Benassi, V.M.; de Lucas, R.C.; Jorge, J.A.; Polizeli, M.L.
Screening of thermotolerant and thermophilic fungi aiming beta-xylosidase and arabinanase production
Braz. J. Microbiol.
45
1459-1467
2014
Aspergillus niger
Manually annotated by BRENDA team
Boyce, A.; Walsh, G.
Purification and characterisation of a thermostable beta-xylosidase from Aspergillus niger van Tieghem of potential application in lignocellulosic bioethanol production
Appl. Biochem. Biotechnol.
186
712-730
2018
Aspergillus niger, Aspergillus niger DSM 22593
Manually annotated by BRENDA team
Patel, H.; Kumar, A.K.; Shah, A.
Purification and characterization of novel bi-functional GH3 family beta-xylosidase/beta-glucosidase from Aspergillus niger ADH-11
Int. J. Biol. Macromol.
109
1260-1269
2018
Aspergillus niger, Aspergillus niger ADH-11
Manually annotated by BRENDA team
Choengpanya, K.; Arthornthurasuk, S.; Wattana-amorn, P.; Huang, W.T.; Plengmuankhae, W.; Li, Y.K.; Kongsaeree, P.T.
Cloning, expression and characterization of beta-xylosidase from Aspergillus niger ASKU28
Protein Expr. Purif.
115
132-140
2015
Aspergillus niger (A0A023J5W7), Aspergillus niger ASKU28 (A0A023J5W7)
Manually annotated by BRENDA team