Information on EC 3.2.1.36 - hyaluronoglucuronidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.36
-
RECOMMENDED NAME
GeneOntology No.
hyaluronoglucuronidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (1->3)-linkages between beta-D-glucuronate and N-acetyl-D-glucosamine residues in hyaluronate
show the reaction diagram
random hydrolysis of 1,3-linkages between beta-D-glucuronate and N-acetyl-D-glucosamine residues in hyaluronate
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
hyaluronate 3-glycanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-34-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
Clostridium welchii
-
-
-
Manually annotated by BRENDA team
Verrill 1872
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-
Manually annotated by BRENDA team
Verrill 1872
-
-
Manually annotated by BRENDA team
Leidy 1870
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-
Manually annotated by BRENDA team
Linnaeus 1758
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-
Manually annotated by BRENDA team
Say 1824
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-
Manually annotated by BRENDA team
Say 1824
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-
Manually annotated by BRENDA team
Linnaeus 1758
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-
Manually annotated by BRENDA team
Linnaeus 1758
-
-
Manually annotated by BRENDA team
Verrill 1872
-
-
Manually annotated by BRENDA team
Verrill 1872
-
-
Manually annotated by BRENDA team
Verrill 1872
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-
Manually annotated by BRENDA team
Verrill 1872
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-
Manually annotated by BRENDA team
Pneumococcus sp.
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
enzyme acts synergistically with vaccinia virus, greatly enhancing the infectivitiy of the virus
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Manually annotated by BRENDA team
strain NCTC 11324
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Manually annotated by BRENDA team
strain NCTC 11324
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-
Manually annotated by BRENDA team
SF370, ATCC700294
UniProt
Manually annotated by BRENDA team
strains A and C
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-
Manually annotated by BRENDA team
Theromyzon sp.
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chondroitin sulfate A + H2O
?
show the reaction diagram
chondroitin sulfate A + H2O
oligosaccharides + ?
show the reaction diagram
chondroitin sulfate C + H2O
?
show the reaction diagram
chondroitin sulfate D + H2O
?
show the reaction diagram
dermatan sulfate + H2O
?
show the reaction diagram
-
chondroitin sulfate B, low activity
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-
?
hyaluronan + H2O
?
show the reaction diagram
hyaluronan + H2O
N-acetyl-beta-D-glucosamine + D-glucuronate
show the reaction diagram
hyaluronic acid + H2O
(N-acetylglucosamine)-(glucuronic acid)-(N-acetylglucosamine)-(glucuronic acid)
show the reaction diagram
hyaluronic acid + H2O
?
show the reaction diagram
hyaluronic acid + H2O
oligosaccharides + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hyaluronic acid + H2O
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
-
strong inhibition at 10 mM
Butane-2,3-dione
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deferoxamine mesylate
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EDTA
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EGTA
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Mn2+
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strong inhibition at 10 mM
Phenylglyoxal
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polymer from gentisic acid
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polymer from hydroxysulfonic acid
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with formaldehyde, nearly complete inhibition at 0.01 mg/ml
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SDS
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complete inhibition at any concentrations
serum protein
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from human, inhibition by a true antibody
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Triton X-100
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moderately inhibits enzyme activity by 3%-30% at different concentrations
Tween 80
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moderately inhibits enzyme activity by 3%-30% at different concentrations
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
hyaluronoic acid
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0.08 mg/ml; value in mg/ml
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
ascorbic acid
Streptococcus pyogenes phage H10403
Q54699
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.027
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head, wet weight
0.028
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crop, wet weight
0.029
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crop, wet weight
0.032
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crop, wet weight
0.038
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crop, wet weight
0.055
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head, wet weight
0.058
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head, wet weight
0.09
Theromyzon sp.
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head and crop, wet weight
0.102
-
head, wet weight
0.111
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head, wet weight
0.117
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crop, wet weight
0.201
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crop, wet weight
0.205
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crop, wet weight
0.278
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head, wet weight
0.325
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crop, wet weight
0.722
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head, wet weight
4.86
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culture supernatant, at pH 6.5 and 44°C
42.05
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head, wet weight
102.1
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after 21.02fold purification, at pH 6.5 and 44°C
additional information
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activity measured as turbidicity reducing units
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 5.8
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
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there is no detectable activity at pH 4.0 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 45
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
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the activity of the enzyme remains high and relatively stable between 40°C and 44°C, with 25% of maximum activity at 46°C. At 60°C or higher, the enzyme loses its activity completely
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
116000
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SDS-PAGE
120000
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2 * 120000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotrimer
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapour diffusion method
hanging-drop vapour-diffusion method
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 6
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the enzyme is most stable at pH 5.0-6.0 and retains more than 90% activity after incubation at this pH range for 2 h
730784
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
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after incubation at 40°C or 45°C for 120 min, the enzyme shows little change with its activity, but its stability is very low at 55°C. The activity is reduced by 99% after incubation at 55°C for 10 min
60
Pneumococcus sp.
-
nearly completely destroyed
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration
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highly purified, chromatography steps
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Ni2+ chelate chromatography, gel filtration
Ni2+-affinity chromatography, gel filtration
to homogeneity, chromatography steps, isoelectric focusing
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged version expressed in Escherichia coli BL21
His-tagged version expressed in Escherichis coli BL21 (DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y264F
involved in catalytic activity, mutant protein without activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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increasing absorption of fluids in dermal clysis, increasing diffusion of injected substances