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Literature summary for 3.2.1.36 extracted from

  • Guo, X.; Shi, Y.; Sheng, J.; Wang, F.
    A novel hyaluronidase produced by Bacillus sp. A50 (2014), PLoS ONE, 9, e94156.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.36

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 100 mM Bacillus sp. (in: Bacteria)
deferoxamine mesylate
-
 Bacillus sp. (in: Bacteria)
EDTA
-
 Bacillus sp. (in: Bacteria)
EGTA
-
 Bacillus sp. (in: Bacteria)
SDS complete inhibition at any concentrations Bacillus sp. (in: Bacteria)
Triton X-100 moderately inhibits enzyme activity by 3%-30% at different concentrations Bacillus sp. (in: Bacteria)
Tween 80 moderately inhibits enzyme activity by 3%-30% at different concentrations Bacillus sp. (in: Bacteria)
Zn2+ strong inhibition at 50 mM Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 154% activity at 100 mM Bacillus sp. (in: Bacteria)
Mg2+ has positive effect on the activity Bacillus sp. (in: Bacteria)
Ni2+ has positive effect on the activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 2 * 120000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) A50
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.86
-
 culture supernatant, at pH 6.5 and 44°C Bacillus sp. (in: Bacteria)
102.1
-
 after 21.02fold purification, at pH 6.5 and 44°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A + H2O 39% activity compared to hyaluronan Bacillus sp. (in: Bacteria) ?
-
 ?
chondroitin sulfate A + H2O 39% activity compared to hyaluronan Bacillus sp. (in: Bacteria) A50 ?
-
 ?
chondroitin sulfate C + H2O 13.5% activity compared to hyaluronan Bacillus sp. (in: Bacteria) ?
-
 ?
chondroitin sulfate C + H2O 13.5% activity compared to hyaluronan Bacillus sp. (in: Bacteria) A50 ?
-
 ?
chondroitin sulfate D + H2O 37.1% activity compared to hyaluronan Bacillus sp. (in: Bacteria) ?
-
 ?
chondroitin sulfate D + H2O 37.1% activity compared to hyaluronan Bacillus sp. (in: Bacteria) A50 ?
-
 ?
hyaluronan + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
hyaluronan + H2O
-
 Bacillus sp. (in: Bacteria) A50 ?
-
 ?
additional information no activity with dermatan sulfate, heparin sodium, heparan sulfate, sodium alginate, chitosan, chitin, sodium carboxymethyl cellulose, dextran, carboxymethyl dextran, xanthan gum, gellan gum, soluble starch, carrageenan, Konjac glucomannan, synanthrin, gumacabic powder, glycogen, or pectin Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with dermatan sulfate, heparin sodium, heparan sulfate, sodium alginate, chitosan, chitin, sodium carboxymethyl cellulose, dextran, carboxymethyl dextran, xanthan gum, gellan gum, soluble starch, carrageenan, Konjac glucomannan, synanthrin, gumacabic powder, glycogen, or pectin Bacillus sp. (in: Bacteria) A50 ?
-
 ?

Subunits

Subunits Comment Organism
? 2 * 120000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
HAase-B
-
 Bacillus sp. (in: Bacteria)
hyaluronidase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
44
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 the activity of the enzyme remains high and relatively stable between 40°C and 44°C, with 25% of maximum activity at 46°C. At 60°C or higher, the enzyme loses its activity completely Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 after incubation at 40°C or 45°C for 120 min, the enzyme shows little change with its activity, but its stability is very low at 55°C. The activity is reduced by 99% after incubation at 55°C for 10 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
4 9 there is no detectable activity at pH 4.0 and pH 9.0 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.9 6 the enzyme is most stable at pH 5.0-6.0 and retains more than 90% activity after incubation at this pH range for 2 h Bacillus sp. (in: Bacteria)