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Information on EC 3.2.1.22 - alpha-galactosidase and Organism(s) Oryza sativa and UniProt Accession Q9FXT4
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3.2.1.22 alpha-galactosidaseIUBMB Comments
Also hydrolyses alpha-D-fucosides.
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Word Map
- 3.2.1.22
- fabry
- lysosomal
-
x-linked
- glycosphingolipids
- cardiac
- globotriaosylceramide
- endothelial
- galactose
- epitope
- oligosaccharide
- pain
- raffinose
- hypertrophy
- ventricular
- proteinuria
- glycosidases
- glycolipids
- angiokeratomas
- alpha-glucosidase
- melibiose
- cerebrovascular
- galactomannans
- simplicifolia
-
multisystemic
-
corporation
-
xenotransplantation
- anaphylaxis
-
hemizygotes
-
cambridge
- alpha-l-fucosidase
- stachyose
- coffee
-
hyperacute
-
anti-gal
- beta-d-glucuronidase
- analysis
-
bandeiraea
- alpha-n-acetylgalactosaminidase
- guar
- beta-d-galactosidase
- biotechnology
-
sanofi
- nutrition
- griffonia
- beta-mannosidase
- imiglucerase
- degradation
- gaucher
- molecular biology
- beta-d-glucosidase
- medicine
- synthesis
-
verticillata
-
soymilk
- alpha-d-mannosidase
- arylamidase
-
flatulence
The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
alpha-galactosidase, alpha-galactosidase a, alpha-gal, alpha-gal a, agalsidase alfa, genzyme, alpha-d-galactosidase, a-galactosidase, fabrazyme, alpha-galactosidase i, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Agalsidase alfa
-
-
-
-
alpha-D-galactosidase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactoside galactohydrolase
ceramidase, galactosylgalactosylglucosyl-
-
-
-
-
ceramide trihexosidase
-
-
-
-
ceramidetrihexosidase
-
-
-
-
ceramidetrihexoside-alpha-galactosidase
-
-
-
-
melibiase
-
-
-
-
trihexosyl ceramide galactosidase
-
-
-
-
trihexosylceramide alpha-galactosidase
-
-
-
-
trihexosylceramidealpha-galactosidase
-
-
-
-
alpha-galactoside galactohydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
CAS REGISTRY NUMBER
COMMENTARY
9023-01-2
-
9025-35-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose
-
-
-
?
O-beta-D-mannopyranosyl-(1-4)-O-[alpha-D-galactopyranosyl-(1-6)]-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
?
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
-
-
-
?
p-nitrophenyl alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
100% activity
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
-
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity
additional information
Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity
additional information
-
Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
p-chloromercuribenzoate
alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h
p-chloromercuribenzoate
alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
AkalphaGal is a thylakoid membrane-degrading enzyme involved in the degradation of digalactosyl diacylglycerol during rice leaf senescence
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor diffusion method, crystal structure determined at 1.5 A resolution
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fujimoto, Z.; Kaneko, S.; Momma, M.; Kobayashi, H.; Mizuno, H.
Crystal structure of rice alpha-galactosidase complexed with D-galactose
J. Biol. Chem.
278
20313-20318
2003
Oryza sativa
Kim, W.D.; Kobayashi, O.; Kaneko, S.; Sakakibara, Y.; Park, G.G.; Kusakabe, I.; Tanaka, H.; Kobayashi, H.
alpha-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells
Phytochemistry
61
621-630
2002
Oryza sativa (Q9FXT4), Oryza sativa
Li, S.; Kim, W.D.; Kaneko, S.; Prema, P.A.; Nakajima, M.; Kobayashi, H.
Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization
Biosci. Biotechnol. Biochem.
71
520-526
2007
Oryza sativa (Q7XIV4), Oryza sativa (Q9FXT4), Oryza sativa
EXTERNAL LINKS (specific for EC-Number 3.2.1.22)
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Release 2023.1 (January 2023)
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