Information on EC 3.2.1.200 - exo-chitinase (non-reducing end)

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The expected taxonomic range for this enzyme is: Pyrococcus furiosus

EC NUMBER
COMMENTARY hide
3.2.1.200
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RECOMMENDED NAME
GeneOntology No.
exo-chitinase (non-reducing end)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of N,N'-diacetylchitobiose from the non-reducing end of chitin and chitodextrins
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chitin degradation I (archaea)
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chitin degradation III (Serratia)
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SYSTEMATIC NAME
IUBMB Comments
(1->4)-2-acetamido-2-deoxy-beta-D-glucan diacetylchitobiohydrolase (non-reducing end)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
colloidal chitin + H2O
N,N'-diacetylchitobiose + ?
show the reaction diagram
-
the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers
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-
?
crab shell chitin + H2O
N,N'-diacetylchitobiose + ?
show the reaction diagram
-
the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers
-
-
?
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O
2 N,N'-diacetylchitobiose
show the reaction diagram
-
the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers
-
-
?
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O
N,N'-diacetylchitobiose + ?
show the reaction diagram
-
the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers
-
-
?
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O
N,N'-diacetylchitobiose + GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta
show the reaction diagram
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ChiBcat hydrolyzes chitooligosaccharides (degree of polymerization = 3 to 6), yielding predominantly chitobiose. (GlcNAc)6 is processively degraded to mostly (GlcNAc)4 and (GlcNAc)2, with limited formation of (GlcNAc)3
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-
?
additional information
?
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no activity with chitobiose and 4-nitrophenyl-N-acetylglucosamine
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
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substrate inhibition
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
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substrate inhibition
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
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substrate inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
pH 6, 95°C
0.3
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
pH 6, 95°C
0.24
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
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pH 6, 95°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13380
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
Pyrococcus furiosus
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pH 6, 95°C
26035
13490
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
Pyrococcus furiosus
-
pH 6, 95°C
28932
8580
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
Pyrococcus furiosus
-
pH 6, 95°C
26036
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.89
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
pH 6, 95°C
2.56
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
pH 6, 95°C
2.15
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
pH 6, 95°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
95
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assay at
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
114
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melting temperature
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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