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Literature summary for 3.2.1.200 extracted from

  • Gao, J.; Bauer, M.W.; Shockley, K.R.; Pysz, M.A.; Kelly, R.M.
    Growth of hyperthermophilic archaeon Pyrococcus furiosus on chitin involves two family 18 chitinases (2003), Appl. Environ. Microbiol., 69, 3119-3128.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.200

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc substrate inhibition Pyrococcus furiosus
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc substrate inhibition Pyrococcus furiosus
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc substrate inhibition Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
0.24
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
0.3
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus N,N'-diacetylchitobiose + ?
-
 ?
crab shell chitin + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus N,N'-diacetylchitobiose + ?
-
 ?
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus 2 N,N'-diacetylchitobiose
-
 ?
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O the enzyme processively cleaves off chitobiose from the nonreducing end of chitin or other chitooligomers Pyrococcus furiosus N,N'-diacetylchitobiose + ?
-
 ?
GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc + H2O ChiBcat hydrolyzes chitooligosaccharides (degree of polymerization = 3 to 6), yielding predominantly chitobiose. (GlcNAc)6 is processively degraded to mostly (GlcNAc)4 and (GlcNAc)2, with limited formation of (GlcNAc)3 Pyrococcus furiosus N,N'-diacetylchitobiose + GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta
-
 ?
additional information no activity with chitobiose and 4-nitrophenyl-N-acetylglucosamine Pyrococcus furiosus ?
-
 ?

Synonyms

Synonyms Comment Organism
ChiB
-
 Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
114
-
 melting temperature Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.15
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
2.56
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
5.89
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8580
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
13380
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus
13490
-
 GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta(1->4)GlcNAc pH 6, 95°C Pyrococcus furiosus