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Information on EC 3.2.1.165 - exo-1,4-beta-D-glucosaminidase and Organism(s) Thermococcus kodakarensis and UniProt Accession Q76HN4

for references in articles please use BRENDA:EC3.2.1.165
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IUBMB Comments
Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi and comprises D-glucosamine residues with a variable content of GlcNAc residues . Acts specifically on chitooligosaccharides and chitosan, having maximal activity on chitotetraose, chitopentaose and their corresponding alcohols . The enzyme can degrade GlcN-GlcNAc but not GlcNAc-GlcNAc . A member of the glycoside hydrolase family 2 (GH-2) .
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Thermococcus kodakarensis
UNIPROT: Q76HN4
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The taxonomic range for the selected organisms is: Thermococcus kodakarensis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
hydrolysis of chitosan or chitosan oligosaccharides to remove successive D-glucosamine residues from the non-reducing termini
Synonyms
exo-beta-d-glucosaminidase, glcnase, gls93, exo-chitosanase, exochitosanase, beta-glcnase, exo-beta-glucosaminidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
exo-beta-D-glucosaminidase
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
chitosan exo-(1->4)-beta-D-glucosaminidase
Chitosan is a partially or totally N-deacetylated chitin derivative that is found in the cell walls of some phytopathogenic fungi and comprises D-glucosamine residues with a variable content of GlcNAc residues [4]. Acts specifically on chitooligosaccharides and chitosan, having maximal activity on chitotetraose, chitopentaose and their corresponding alcohols [1]. The enzyme can degrade GlcN-GlcNAc but not GlcNAc-GlcNAc [3]. A member of the glycoside hydrolase family 2 (GH-2) [4].
CAS REGISTRY NUMBER
COMMENTARY hide
9012-33-3
c.f. EC 3.2.1.52
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl beta-D-glucoside + H2O
4-methylumbelliferol + D-glucose
show the reaction diagram
-
-
-
?
chitobiose + H2O
2 D-glucosamine
show the reaction diagram
-
-
-
?
chitobiose + H2O
D-glucosamine
show the reaction diagram
-
-
-
?
chitohexaose + H2O
D-glucosamine
show the reaction diagram
at the early stages of the reaction, chitohexaose is converted to D-glucosamine and chitopentaose
-
-
?
chitopentaose + H2O
D-glucosamine
show the reaction diagram
at the early stages of the reaction, chitopentaose is converted to D-glucosamine and chitotetraose
-
-
?
chitotetraose + H2O
D-glucosamine
show the reaction diagram
at the early stages of the reaction, chitotetraose is converted to D-glucosamine and chitotriose
-
-
?
chitotriose + H2O
D-glucosamine
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.37
chitobiose
pH 6.0, 80°C
0.365
chitohexaose
pH 6.0, 80°C
0.0778
chitopentaose
pH 6.0, 80°C
0.295
chitotetraose
pH 6.0, 80°C
0.27
chitotriose
pH 6.0, 80°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 100
37°C: about 20% of maximal activity, 100°C: about 20% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
193000
gel filtration
86000
2 * 86000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 86000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E179Q
the mutant shows 3% of wild type activity
E306Q
the mutant shows 40% of wild type activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3)pLysS cells
expression in Escherichia coli BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tanaka, T.; Fukui, T.; Atomi, H.; Imanaka, T.
Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1
J. Bacteriol.
185
5175-5181
2003
Thermococcus kodakarensis (Q76HN4)
Manually annotated by BRENDA team
Mine, S.; Watanabe, M.; Kamachi, S.; Abe, Y.; Ueda, T.
The structure of an archaeal beta-glucosaminidase provides insight into glycoside hydrolase evolution
J. Biol. Chem.
292
4996-5006
2017
Pyrococcus horikoshii, Thermococcus kodakarensis (Q76HN4)
Manually annotated by BRENDA team