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Literature summary for 3.2.1.165 extracted from
- Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2003), J. Bacteriol., 185, 5175-5181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0778 | - |
chitopentaose | pH 6.0, 80°C | Thermococcus kodakarensis |  |
| 0.27 | - |
chitotriose | pH 6.0, 80°C | Thermococcus kodakarensis | |
| 0.295 | - |
chitotetraose | pH 6.0, 80°C | Thermococcus kodakarensis | |
| 0.365 | - |
chitohexaose | pH 6.0, 80°C | Thermococcus kodakarensis | |
| 1.37 | - |
chitobiose | pH 6.0, 80°C | Thermococcus kodakarensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 86000 | - |
2 * 86000, SDS-PAGE | Thermococcus kodakarensis |
| 193000 | - |
gel filtration | Thermococcus kodakarensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q76HN4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-glucoside + H2O | - |
Thermococcus kodakarensis | 4-methylumbelliferol + D-glucose | - |
? | |
| chitobiose + H2O | - |
Thermococcus kodakarensis | D-glucosamine | - |
? | |
| chitohexaose + H2O | at the early stages of the reaction, chitohexaose is converted to D-glucosamine and chitopentaose | Thermococcus kodakarensis | D-glucosamine | - |
? | |
| chitopentaose + H2O | at the early stages of the reaction, chitopentaose is converted to D-glucosamine and chitotetraose | Thermococcus kodakarensis | D-glucosamine | - |
? | |
| chitotetraose + H2O | at the early stages of the reaction, chitotetraose is converted to D-glucosamine and chitotriose | Thermococcus kodakarensis | D-glucosamine | - |
? | |
| chitotriose + H2O | - |
Thermococcus kodakarensis | D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 86000, SDS-PAGE | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-beta-D-glucosaminidase | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermococcus kodakarensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 100 | 37°C: about 20% of maximal activity, 100°C: about 20% of maximal activity | Thermococcus kodakarensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermococcus kodakarensis |
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Release 2023.1 (January 2023)
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