Information on EC 3.2.1.120 - oligoxyloglucan beta-glycosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.120
-
RECOMMENDED NAME
GeneOntology No.
oligoxyloglucan beta-glycosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-beta-D-glucosidic links in oligoxyloglucans so as to remove successive isoprimeverose [i.e. alpha-xylo-(1->6)-beta-D-glucosyl-] residues from the non-reducing chain ends
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
xyloglucan degradation II (exoglucanase)
-
-
SYSTEMATIC NAME
IUBMB Comments
oligoxyloglucan xyloglucohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
97162-80-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
M9
-
-
Manually annotated by BRENDA team
M9
-
-
Manually annotated by BRENDA team
Malus malus
var. Golden delicious
-
-
Manually annotated by BRENDA team
Y1
-
-
Manually annotated by BRENDA team
Y1
-
-
Manually annotated by BRENDA team
M451
-
-
Manually annotated by BRENDA team
M451
-
-
Manually annotated by BRENDA team
auxin-treated
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + Xylbeta(1-6)Glcbeta(1-4)Glc
show the reaction diagram
apple unextractable solid + H2O
oligosaccharide + ?
show the reaction diagram
Malus malus
-
-
-
-
?
azo-cellulose + H2O
oligosaccharide + ?
show the reaction diagram
-
-
-
?
azuki bean cell wall + H2O
fragments of 50000 Da + ?
show the reaction diagram
-
-
-
-
?
beta-glucan + H2O
oligosaccharide + ?
show the reaction diagram
-
-
-
?
chemically modified cellulose + H2O
oligosaccharide + ?
show the reaction diagram
tamarind xyloglucan + H2O
oligosaccharide + ?
show the reaction diagram
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + H2O
beta-D-xylose + (Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
show the reaction diagram
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc + isoprimeverose
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
show the reaction diagram
Xylbeta(1-6)Glcbeta(1-4)Glc + H2O
Xylbeta(1-6)Glc + beta-D-glucopyranose
show the reaction diagram
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
xyloglucan fragment oligosaccharide + H2O
isoprimeverose
show the reaction diagram
-
highly specific for
isoprimeverose is split off from the nonreducing end of the backbone of the substrate
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024
tamarind xyloglucan
-
50°C, pH 7.5
-
0.7
Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
-
-
0.966
xyloglucan oligosaccharide
-
50°C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1
-
pH 4.5, 40°C, substrate: beta-glucan; pH 4.5, 40°C, substrate: chemically modified cellulose
19
-
pH 4.5, 40°C, substrate: tamarind xyloglucan
85
-
with Xylbeta(1-6)Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)(Xylbeta(1-6))Glcbeta(1-4)Glc
260
-
21°C, pH 5.0
1294
-
pH 6.2, 35°C
additional information
-
overview: assay methods
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 7.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.9
-
isoelectric focusing
8.1
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chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23600
-
x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence
23760
-
x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence
32000
-
x * 32000, SDS-PAGE
34000
-
x * 23600, mass spectroscopy, x * 34000, SDS-PAGE, x * 23760, deduced from gene sequence
70000
-
gel filtration
77000
-
1 * 77000, SDS-PAGE
79440
-
x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence
79480
-
x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence
105000
-
x * 105000, SDS-PAGE
115000
-
2 * 115000, SDS-PAGE
230000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 115000, SDS-PAGE
monomer
-
1 * 77000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
-
no N-glycosylation site
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 7.5
-
-
680407
5 - 7
-
45°C, 10 min, stable
646669
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
145 h, 90% residual activity
45
-
more than 90% of the enzyme activity retained
60
-
16 h, 75% residual activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very stable below 35°C
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by anion-exchange chromatography and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Aspergillus oryzae
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expression in Escherichia coli
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overview: gene family
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology