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Literature summary for 3.2.1.120 extracted from

  • Irwin, D.C.; Cheng, M.; Xiang, B.; Rose, J.K.C.; Wilson, D.B.
    Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca (2003), Eur. J. Biochem., 270, 3083-3091.
    View publication on PubMed
Search for more literature for 3.2.1.120

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 tamarind xyloglucan 50°C, pH 7.5 Thermobifida fusca
0.966
-
 xyloglucan oligosaccharide 50°C, pH 7.5 Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79440
-
 x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence Thermobifida fusca
79480
-
 x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermobifida fusca enzyme allows for use of cellulose as carbon source by breaking down surrounding cellulose fibrils ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Reaction

Reaction Comment Organism Reaction ID
R1-Glcbeta(1-4)Glcbeta[Xylbeta(1-6)Glcbeta(1-4)](1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + H2O = R1-Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose inverting mechanism Thermobifida fusca
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: carboxymethylcellulose, swollen cellulose Thermobifida fusca ?
-
 ?
additional information enzyme allows for use of cellulose as carbon source by breaking down surrounding cellulose fibrils Thermobifida fusca ?
-
 ?
tamarind xyloglucan + H2O
-
 Thermobifida fusca oligosaccharide + ?
-
 ?
xyloglucan fragment oligosaccharide + H2O
-
 Thermobifida fusca isoprimeverose
-
 ?

Subunits

Subunits Comment Organism
? x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 16 h, full activity Thermobifida fusca
60
-
 16 h, 75% residual activity Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
6 9.4
-
 Thermobifida fusca