Information on EC 3.1.3.68 - 2-deoxyglucose-6-phosphatase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY
3.1.3.68
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RECOMMENDED NAME
GeneOntology No.
2-deoxyglucose-6-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-Deoxy-D-glucose 6-phosphate + H2O = 2-deoxy-D-glucose + phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
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SYSTEMATIC NAME
IUBMB Comments
2-deoxy-D-glucose-6-phosphate phosphohydrolase
Also active towards fructose 1-phosphate
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2-deoxy-glucose 6-phosphate phosphatase
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2-Deoxyglucose 6-phosphate phosphatase
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2-Deoxyglucose-6-phosphate phosphatase
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2-DOG-6P phosphatase
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GenBank AE000318-derived protein GI 1788630
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GenBank D90861-derived protein GI 1799667
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Phosphatase, 2-deoxyglucose 6-
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Phosphatase, 2-deoxyglucose 6- (Escherichia coli strain K-12 Kohara clone 405 gene DOG1)
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Phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae clone pGEMT-DOGR1)
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Phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae gene DOGR1 isoform)
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Phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae gene DOGR1)
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Phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae gene DOGR2 isoform)
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Protein (Escherichia coli strain K12-MG1655 gene b2293)
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CAS REGISTRY NUMBER
COMMENTARY
159036-75-6
phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae gene DOGR1) /phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae clone pGEMT-DOGR1) /phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae gene DOGR1 isoform)
172400-89-4
phosphatase, 2-deoxyglucose 6- (Saccharomyces cerevisiae gene DOGR2 isoform)
1923393-20-7
phosphatase, 2-deoxyglucose 6- (Escherichia coli strain K-12 Kohara clone 405 gene DOG1) /genBank AE000318-derived protein GI 1788630 / /genBank D90861-derived protein GI 1799667 /protein (Escherichia coli strain K12-MG1655 gene b2293)
65187-56-6
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 10a12-13; strain PM-II; the enzyme is also present in wild type yeast, although its level is ten to twenty times lower than those found in the mutant
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Manually annotated by BRENDA team
the enzyme is also present in wild type yeast, although its level is ten to twenty times lower than those found in the mutant
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Manually annotated by BRENDA team
Saccharomyces cerevisiae 10a12-13
strain 10a12-13
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Manually annotated by BRENDA team
Saccharomyces cerevisiae PM-II
strain PM-II
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Deoxy-D-glucose 6-phosphate + H2O
2-Deoxy-D-glucose + phosphate
show the reaction diagram
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2-Deoxy-D-glucose 6-phosphate + H2O
2-Deoxy-D-glucose + phosphate
show the reaction diagram
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?
2-Deoxy-D-glucose 6-phosphate + H2O
?
show the reaction diagram
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the expression of the enzyme is increased by the presence of 2-deoxyglucose and is constant along the growth curve
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2-Deoxy-D-glucose 6-phosphate + H2O
?
show the reaction diagram
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the enzyme may be involved in inhibiting the process of catabolite repression mediated by 2-deoxyglucose
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2-Deoxyribose 5-phosphate + H2O
2-Deoxyribose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 21% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 1% of the activity with 2-deoxy-D-glucose 6-phosphate
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Arabinose 5-phosphate + H2O
Arabinose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 6% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 6% of the activity with 2-deoxy-D-glucose 6-phosphate
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D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 2% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 17% of the activity with 2-deoxy-D-glucose 6-phosphate
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D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
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?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 42% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 13% of the activity with 2-deoxy-D-glucose 6-phosphate
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D-ribulose 5-phosphate + H2O
D-ribulose + phosphate
show the reaction diagram
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?
D-xylulose 5-phosphate + H2O
D-xylulose + phosphate
show the reaction diagram
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?
Fructose 1-phosphate + H2O
Fructose + phosphate
show the reaction diagram
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with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
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Fructose 1-phosphate + H2O
Fructose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 12% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 106% of the activity with 2-deoxy-D-glucose 6-phosphate
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Fructose 1-phosphate + H2O
Fructose + phosphate
show the reaction diagram
Saccharomyces cerevisiae 10a12-13, Saccharomyces cerevisiae PM-II
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with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
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Glucose 6-phosphate + H2O
Glucose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 20% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 30% of the activity with 2-deoxy-D-glucose 6-phosphate
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Ribulose 5-phosphate + H2O
Ribulose + phosphate
show the reaction diagram
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isoenzyme DogR1p: at 7% of the activity with 2-deoxy-D-glucose 6-phosphate, isoenzyme DogR2p: at 1% of the activity with 2-deoxy-D-glucose 6-phosphate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-Deoxy-D-glucose 6-phosphate + H2O
?
show the reaction diagram
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the expression of the enzyme is increased by the presence of 2-deoxyglucose and is constant along the growth curve
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2-Deoxy-D-glucose 6-phosphate + H2O
?
show the reaction diagram
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the enzyme may be involved in inhibiting the process of catabolite repression mediated by 2-deoxyglucose
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METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Cd2+
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can replace Mg2+ in activation, with less than 15% of the efficiency
Co2+
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can replace Mg2+ in activation, with 65% of the efficiency
Mg2+
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required, maximal activation with 10 mM
Mn2+
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can replace Mg2+ in activation, with less than 15% of the efficiency
Ni2+
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can replace Mg2+ in activation, with 35% of the efficiency
Zn2+
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can replace Mg2+ in activation, with less than 15% of the efficiency
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
fructose 1-phosphate
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
15
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2-deoxy-D-glucose 6-phosphate
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17
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2-deoxy-D-glucose 6-phosphate
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isoenzyme DogR1p
41
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2-deoxy-D-glucose 6-phosphate
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isoenzyme DogR2p
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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additional information
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2-deoxy-glucose 6-phosphate (100%), D-ribulose 5-phosphate (7%), D-ribose 5-phosphate (42%), D-xylulose 5-phosphate (15%)
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
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isoenzyme DogR1p and DogR2p
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8
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50% of maximal activity at pH 5 and 8
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
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isoenzyme DogR1p
40
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isoenzyme DogR2p
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
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molecular characterization of the gene
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
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pH 7, 5 min, , 20% loss of activity
40
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pH 7, nearly complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-10°C, stable for several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2 isoenzymes: DogR1p and DogR2p
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
overexpression of DOG1 in Saccharomyces cerevisiae transketolase-deficient strain H1520 containing the integrated xylitol dehydrogenase-encoding gene (XYL2) gene increases the excretion of ribitol (by 1.6fold) and D-ribose (by 3.8fold) compared to the strain lacking DOG1
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