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Literature summary for 3.1.3.68 extracted from
- Characterization of a phosphatase specific for 2-deoxyglucose-6-phosphate in a yeast mutant (1977), FEBS Lett., 83, 245-248.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fructose 1-phosphate | - |
Saccharomyces cerevisiae |  |
| phosphate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
2-deoxy-D-glucose 6-phosphate | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | can replace Mg2+ in activation, with less than 15% of the efficiency | Saccharomyces cerevisiae | |
| Co2+ | can replace Mg2+ in activation, with 65% of the efficiency | Saccharomyces cerevisiae | |
| Mn2+ | can replace Mg2+ in activation, with less than 15% of the efficiency | Saccharomyces cerevisiae | |
| Ni2+ | can replace Mg2+ in activation, with 35% of the efficiency | Saccharomyces cerevisiae | |
| Zn2+ | can replace Mg2+ in activation, with less than 15% of the efficiency | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | - |
the enzyme is also present in wild type yeast, although its level is ten to twenty times lower than those found in the mutant | - |
| Saccharomyces cerevisiae 10a12-13 | - |
- |
- |
| Saccharomyces cerevisiae PM-II | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, stable for several weeks | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fructose 1-phosphate + H2O | with 80% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae | Fructose + phosphate | - |
? | |
| Fructose 1-phosphate + H2O | with 80% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae 10a12-13 | Fructose + phosphate | - |
? | |
| Fructose 1-phosphate + H2O | with 80% of the activity with 2-deoxy-D-glucose 6-phosphate | Saccharomyces cerevisiae PM-II | Fructose + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
pH 7, 5 min, , 20% loss of activity | Saccharomyces cerevisiae |
| 40 | - |
pH 7, nearly complete loss of activity | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | 50% of maximal activity at pH 5 and 8 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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