Information on EC 3.1.3.27 - phosphatidylglycerophosphatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.1.3.27
-
RECOMMENDED NAME
GeneOntology No.
phosphatidylglycerophosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cardiolipin biosynthesis
-
-
cardiolipin biosynthesis I
-
-
cardiolipin biosynthesis II
-
-
cardiolipin biosynthesis III
-
-
Glycerophospholipid metabolism
-
-
lipid metabolism
-
-
Metabolic pathways
-
-
phosphatidylglycerol biosynthesis I (plastidic)
-
-
phosphatidylglycerol biosynthesis II (non-plastidic)
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylglycerophosphate phosphohydrolase
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PGP phosphatase
-
-
-
-
phosphatidylglycero phosphate phosphatase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9033-46-9
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PCC7120
-
-
Manually annotated by BRENDA team
Anabaena sp. PCC7120
strain PCC7120
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
Micrococcus cerificans
-
-
-
Manually annotated by BRENDA team
bakers yeast
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
phosphatidylglycerophosphate phosphatase essential for the biosynthesis of cardiolipin, an integral component of mitochondrial and bacterial membranes
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dioctanoyl-sn-glycero-3-diphosphate + H2O
?
show the reaction diagram
C6EFV6
-
-
-
?
1,2-dioleoyl-sn-glycero-3-diphosphate + H2O
?
show the reaction diagram
C6EFV6
-
-
-
?
1,2-dioleoyl-sn-glycero-3-phosphate + H2O
1,2-dioleoyl-sn-glycerol + phosphate
show the reaction diagram
C6EFV6
highest activity
-
-
?
1-oleoyl-2-lyso-sn-glycero-3-phosphate + H2O
1-oleoyl-2-lyso-sn-glycerol + phosphate
show the reaction diagram
C6EFV6
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
dipalmitoyl sn-glycero 3-phosphate + H2O
dipalmitoyl sn-glycerol + phosphate
show the reaction diagram
-
-
-
r
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
Anabaena sp. PCC7120
-
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
r
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
Micrococcus cerificans
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
r
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
hydrolysis of other simple phosphomonoesters not catalyzed
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
Micrococcus cerificans
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
sn-phosphatidyl 1-sn-glycero 3-phosphate + H2O
3-phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
undecaprenyl diphosphate + H2O
?
show the reaction diagram
C6EFV6
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidylglycerophosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
Micrococcus cerificans
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
undecaprenyl diphosphate + H2O
?
show the reaction diagram
C6EFV6
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
each protomer contains an independent active site with two metal ions, Ca2+ and Mg2+, forming a heterobinuclear center located in a hydrophilic cavity near the surface of the molecule
Mg2+
-
each protomer contains an independent active site with two metal ions, Ca2+ and Mg2+, forming a heterobinuclear center located in a hydrophilic cavity near the surface of the molecule
Mg2+
-
highest activity with 1 mM
Mg2+
-
4 mM, not required for PGPB-tase
Mg2+
-
addition of high concentrated divalent cations not required
Mg2+
-
5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ca2+
-
-
Ca2+
-
-
Co2+
-
-
Co2+
-
-
Cu2+
-
-
EDTA
-
higher concentrations
Hg2+
-
complete inhibition at 0.5 mM
Hg2+
-
complete inhibition at 1 mM
iodoacetamide
-
-
ionic and non-ionic detergents
-
most effective is Triton X-100
-
Mg2+
-
-
Mn2+
-
higher than 1 mM
Mn2+
-
-
N-ethylmaleimide
-
-
NaF
-
80% loss of activity with 5 mM in compound fraction of PGP-A, PGP-B and PGP-C
NaF
-
70% inhibition at 1 mM
NaF
-
-
Octadecylamine
-
-
octyl-beta-D-glucopyranoside
-
complete inhibition with 30 mM
oleic acid
-
higher than 1.5 mM
p-Chloromercuriphenylsulfonic acid
-
100% inhibition at 1 mM
p-mercuribenzoic acid
-
-
phosphate
-
-
phosphate
-
little effect up to 10 mM
phosphatidic acid ammonium salt
-
3.0 mM
phosphatidylglycerophosphate
-
more than 100 M
Sulfhydryl inhibitors
-
-
-
trimethyloctadecyl ammonium chloride
-
-
Triton X-100
-
only in heat treatment
Triton X-100
-
-
Triton X-100
-
high concentration
Zn2+
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
arachidonic acid
-
-
EDTA
-
1 mM
EDTA
-
0.01 - 0.10 mM
EDTA
-
distinctly stimulatory at 1 mM
EGTA
-
0.01 - 0.10 mM
oleic acid
-
1.9 fold stimulation at 0.5 mM
phosphatidic acid sodium salt
-
2 fold stimulation at 3.0 mM
Triton X-100
-
-
Triton X-100
-
up to 5 mM
Triton X-100
-
-
Triton X-100
-
optimal at 1-1.5 mg/ml
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
1,2-dioleoyl-sn-glycero-3-phosphate
C6EFV6
pH and temperature not specified in the publication
0.012
dipalmitoyl sn-glycero 3-phosphate
-
-
0.002
phosphatidylglycerophosphate
-
-
0.015
phosphatidylglycerophosphate
-
-
0.08
phosphatidylglycerophosphate
-
-
0.083
phosphatidylglycerophosphate
-
-
0.18
phosphatidylglycerophosphate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
24
1,2-dioleoyl-sn-glycero-3-phosphate
C6EFV6
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0004
-
-
0.0017
-
partially purified
0.0018
-
pgpB geneproduct
0.0021
-
pgpA geneproduct
0.0023
-
partially purified
0.01
-
PGP-C
0.014
-
compound of PGP-A,-B and -C
0.028
-
partially purified
0.13
-
PGP-B and -C
0.19
-
PGP-A and -C
5.2
-
PGPC-tase
6
-
PGPA-tase, more specific for phosphatidylglycerolphosphate than PGPB-tase
10.1
-
PGPB-tase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.3
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 8
-
little activity below pH 7.0 and above pH 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain B / BL21-DE3)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
C6EFV6
x * 29000, SDS-PAGE
homotetramer
-
x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method
C6EFV6
with precipitant containing 30% pentaerythritol ethoxylate, 50 mM ammonium sulfate, 50 mM Bis-Tris at pH 6.5
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
-
95% activity for several weeks, stored in liquid nitrogen
94818
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
43
C6EFV6
the melting temperature of the wild type enzyme is 43C
730834
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% loss of activity after solubilization with Triton X-100 in 12 h
-
PGP-C more sensitive to increasing temperature
-
presence of dithiothreitol required
-
resistant to solubilization with Triton X-100 if 5 mM EDTA is included
-
sensitive to sulfhydryl reagents and freeze-thaw cycles
-
the enzyme is stabilized by Na2MoO4 but not by Na2SO4
C6EFV6
unstable at room temperature
-
heat labile
-
PGP-C more sensitive to increasing temperature
-
sensitive to sulfhydryl reagents and freeze-thaw cycles
-
heat stable up to 50C
-
inactivation above 60C
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Triton X-100
-
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, for weeks in 2% Triton X-100, 50 mM MgCl2, 20% glycerol, 0.5 M KCl
-
-20C, stable for weeks
-
-20C, stable for several months in 0.25 sucrose, 0.1 mM EDTA, pH 7.4
-
4C, stable for a number of weeks in 20% glycerol, 0.01 M Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM beta-mercaptoethanol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified in a detergent combination of nonyl-beta-D-glucopyranoside and n-dodecyl-N,N-dimethylamine-N-oxide
C6EFV6
partial
-
GST-bind resin column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Escherichia coli strains lacking the original pgpA and/or pgpB genes
-
expressed in Escherichia coli
C6EFV6
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21 (DE3) CodonPlus RIPL cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A83F
C6EFV6
the mutant shows reduced activity
D211E
C6EFV6
the mutant shows strongly reduced activity
F61W
C6EFV6
the mutant shows reduced activity
G89L
C6EFV6
the mutant shows strongly reduced activity
G91M
C6EFV6
the mutant shows reduced activity
H163A
C6EFV6
the mutant shows strongly reduced activity
H207A
C6EFV6
the mutant shows strongly reduced activity
H57F
C6EFV6
the mutant shows about wild type activity
K93E
C6EFV6
the mutant shows reduced activity
K97A
C6EFV6
the mutant shows strongly reduced activity
L58F
C6EFV6
the mutant shows about wild type activity
Q90A
C6EFV6
the mutant shows reduced activity
R104A
C6EFV6
the mutant shows strongly reduced activity
R201A
C6EFV6
the mutant shows strongly reduced activity
V54F
C6EFV6
the mutant shows reduced activity
V88F
C6EFV6
the mutant shows reduced activity
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
preparation of phosphatidylglycerophosphate