Information on EC 3.1.3.27 - phosphatidylglycerophosphatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.1.3.27
-
RECOMMENDED NAME
GeneOntology No.
phosphatidylglycerophosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
cardiolipin biosynthesis I
-
cardiolipin biosynthesis II
-
cardiolipin biosynthesis III
-
Glycerophospholipid metabolism
-
Metabolic pathways
-
phosphatidylglycerol biosynthesis I (plastidic)
-
phosphatidylglycerol biosynthesis II (non-plastidic)
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylglycerophosphate phosphohydrolase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
PGP phosphatase
-
-
-
-
PGP phosphatase
-
-
PGP phosphatase
Anabaena sp. PCC7120
-
-
-
PGP-tase
-
-
PGPA-tase
-
-
PGPase
-
-
PGPB-tase
-
-
PGPC-tase
-
-
phosphatidylglycero phosphate phosphatase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9033-46-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain PCC7120
-
-
Manually annotated by BRENDA team
Anabaena sp. PCC7120
strain PCC7120
-
-
Manually annotated by BRENDA team
Syrian hamster
-
-
Manually annotated by BRENDA team
Micrococcus cerificans
-
-
-
Manually annotated by BRENDA team
bakers yeast
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dipalmitoyl sn-glycero 3-phosphate + H2O
dipalmitoyl sn-glycerol + phosphate
show the reaction diagram
-
-
-
r
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
r
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
Micrococcus cerificans
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
r
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
phosphatidylglycero 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
hydrolysis of other simple phosphomonoesters not catalyzed
-
-
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
-
?
phosphatidylglycerol 3-phosphate + H2O
phosphatidylglycerol + phosphate
show the reaction diagram
Anabaena sp. PCC7120
-
-
-
-
?
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
Micrococcus cerificans
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
sn-phosphatidyl 1-sn-glycero 3-phosphate + H2O
3-phosphatidylglycerol + phosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
-
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
Micrococcus cerificans
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
phosphatidylglycerophosphate + H2O
?
show the reaction diagram
-
biosynthesis of phospholipids
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
each protomer contains an independent active site with two metal ions, Ca2+ and Mg2+, forming a heterobinuclear center located in a hydrophilic cavity near the surface of the molecule
Mg2+
-
each protomer contains an independent active site with two metal ions, Ca2+ and Mg2+, forming a heterobinuclear center located in a hydrophilic cavity near the surface of the molecule
Mg2+
-
highest activity with 1 mM
Mg2+
-
4 mM, not required for PGPB-tase
Mg2+
-
addition of high concentrated divalent cations not required
Mg2+
-
5 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
higher concentrations
Hg2+
-
complete inhibition at 0.5 mM
Hg2+
-
complete inhibition at 1 mM
iodoacetamide
-
-
ionic and non-ionic detergents
-
most effective is Triton X-100
-
Mn2+
-
higher than 1 mM
N-ethylmaleimide
-
-
NaF
-
80% loss of activity with 5 mM in compound fraction of PGP-A, PGP-B and PGP-C
NaF
-
70% inhibition at 1 mM
Octadecylamine
-
-
octyl-beta-D-glucopyranoside
-
complete inhibition with 30 mM
oleic acid
-
higher than 1.5 mM
p-Chloromercuriphenylsulfonic acid
-
100% inhibition at 1 mM
p-mercuribenzoic acid
-
-
phosphate
-
little effect up to 10 mM
phosphatidic acid ammonium salt
-
3.0 mM
phosphatidylglycerophosphate
-
more than 100 M
Sulfhydryl inhibitors
-
-
-
trimethyloctadecyl ammonium chloride
-
-
-
Triton X-100
-
only in heat treatment
Triton X-100
-
-
Triton X-100
-
high concentration
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
arachidonic acid
-
-
EDTA
-
0.01 - 0.10 mM
EDTA
-
distinctly stimulatory at 1 mM
EGTA
-
0.01 - 0.10 mM
oleic acid
-
1.9 fold stimulation at 0.5 mM
phosphatidic acid sodium salt
-
2 fold stimulation at 3.0 mM
Triton X-100
-
-
Triton X-100
-
up to 5 mM
Triton X-100
-
optimal at 1-1.5 mg/ml
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.012
-
dipalmitoyl sn-glycero 3-phosphate
-
-
0.002
-
phosphatidylglycerophosphate
-
-
0.015
-
phosphatidylglycerophosphate
-
-
0.08
-
phosphatidylglycerophosphate
-
-
0.083
-
phosphatidylglycerophosphate
-
-
0.18
-
phosphatidylglycerophosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0004
-
-
-
0.0017
-
-
partially purified
0.0018
-
-
pgpB geneproduct
0.0021
-
-
pgpA geneproduct
0.0023
-
-
partially purified
0.01
-
-
PGP-C
0.014
-
-
compound of PGP-A,-B and -C
0.028
-
-
partially purified
0.13
-
-
PGP-B and -C
0.19
-
-
PGP-A and -C
5.2
-
-
PGPC-tase
6
-
-
PGPA-tase, more specific for phosphatidylglycerolphosphate than PGPB-tase
10.1
-
-
PGPB-tase
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.3
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
8
-
little activity below pH 7.0 and above pH 8.0
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Escherichia coli (strain B / BL21-DE3)
Neisseria meningitidis serogroup B (strain MC58)
Neisseria meningitidis serogroup B (strain MC58)
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
homotetramer
-
x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
with precipitant containing 30% pentaerythritol ethoxylate, 50 mM ammonium sulfate, 50 mM Bis-Tris at pH 6.5
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
95% activity for several weeks, stored in liquid nitrogen
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
50% loss of activity after solubilization with Triton X-100 in 12 h
-
PGP-C more sensitive to increasing temperature
-
presence of dithiothreitol required
-
resistant to solubilization with Triton X-100 if 5 mM EDTA is included
-
sensitive to sulfhydryl reagents and freeze-thaw cycles
-
unstable at room temperature
-
heat labile
-
PGP-C more sensitive to increasing temperature
-
sensitive to sulfhydryl reagents and freeze-thaw cycles
-
heat stable up to 50C
-
inactivation above 60C
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Triton X-100
-
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, for weeks in 2% Triton X-100, 50 mM MgCl2, 20% glycerol, 0.5 M KCl
-
-20C, stable for weeks
-
-20C, stable for several months in 0.25 sucrose, 0.1 mM EDTA, pH 7.4
-
4C, stable for a number of weeks in 20% glycerol, 0.01 M Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM beta-mercaptoethanol
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Escherichia coli strains lacking the original pgpA and/or pgpB genes
-
expressed in Escherichia coli BL21 (DE3) cells
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
preparation of phosphatidylglycerophosphate