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Literature summary for 3.1.3.27 extracted from
- Phosphatidylglycerophosphate phosphatase from Escherichia coli (1992), Methods Enzymol., 209, 224-230.
General Stability
| General Stability | Organism |
|---|---|
| heat labile | Escherichia coli |
| PGP-C more sensitive to increasing temperature | Escherichia coli |
| presence of dithiothreitol required | Escherichia coli |
| resistant to solubilization with Triton X-100 if 5 mM EDTA is included | Escherichia coli |
| sensitive to sulfhydryl reagents and freeze-thaw cycles | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaF | 80% loss of activity with 5 mM in compound fraction of PGP-A, PGP-B and PGP-C | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
phosphatidylglycerophosphate | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| inner membrane | - |
Escherichia coli | - |
- |
| membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylglycerophosphate + H2O | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
PGP-C | Escherichia coli |
| 0.014 | - |
compound of PGP-A,-B and -C | Escherichia coli |
| 0.13 | - |
PGP-B and -C | Escherichia coli |
| 0.19 | - |
PGP-A and -C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylglycerophosphate + H2O | - |
Escherichia coli | phosphatidylglycerol + phosphate | - |
? | |
| phosphatidylglycerophosphate + H2O | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGP-A | - |
Escherichia coli |
| PGP-B | - |
Escherichia coli |
| PGP-C | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Escherichia coli |
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