Information on EC 3.1.26.1 - Physarum polycephalum ribonuclease

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The expected taxonomic range for this enzyme is: Physarum polycephalum

EC NUMBER
COMMENTARY hide
3.1.26.1
-
RECOMMENDED NAME
GeneOntology No.
Physarum polycephalum ribonuclease
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endonucleolytic cleavage to 5'-phosphomonoester
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
CAS REGISTRY NUMBER
COMMENTARY hide
9001-99-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ApA + H2O
5'-AMP + adenosine
show the reaction diagram
-
-
-
-
?
ApC + H2O
5'-CMP + adenosine
show the reaction diagram
-
-
-
-
?
ApG + H2O
5'-GMP + adenosine
show the reaction diagram
-
-
-
-
?
ApU + H2O
5'-UMP + adenosine
show the reaction diagram
-
-
-
-
?
CpA + H2O
5'-AMP + cytidine
show the reaction diagram
-
-
-
-
?
CpC + H2O
5'-CMP + cytidine
show the reaction diagram
-
-
-
-
?
CpG + H2O
5'-GMP + cytidine
show the reaction diagram
-
-
-
-
?
CpU + H2O
5'-UMP + cytidine
show the reaction diagram
-
-
-
-
?
DNA + H2O
nucleoside 5'-monophosphates
show the reaction diagram
-
-
-
?
GpA + H2O
5'-AMP + guanosine
show the reaction diagram
-
-
-
-
?
GpC + H2O
5'-CMP + guanosine
show the reaction diagram
-
-
-
-
?
GpG + H2O
5'-GMP + guanosine
show the reaction diagram
-
-
-
-
?
GpU + H2O
5'-UMP + guanosine
show the reaction diagram
-
-
-
-
?
poly(I) + H2O
5'-IMP
show the reaction diagram
-
-
-
-
?
polyA + H2O
AMP
show the reaction diagram
-
-
-
-
?
polyC + H2O
5'CMP
show the reaction diagram
-
-
-
-
?
polyU + H2O
5'UMP
show the reaction diagram
-
-
-
-
?
RNA + H2O
5'-phosphonucleotide
show the reaction diagram
-
low molecular weigt yeast RNA
product identification
-
?
RNA + H2O
mononucleotides
show the reaction diagram
-
yeast RNA, base specificity of B1 site is A,G,U > C and of B2 A > G > C > U, depolymerization
-
-
?
RNA + H2O
nucleoside 5'-monophosphates
show the reaction diagram
-
-
-
?
UpA + H2O
5'-AMP + uridine
show the reaction diagram
-
-
-
-
?
UpC + H2O
5'-CMP + uridine
show the reaction diagram
-
-
-
-
?
UpG + H2O
5'-GMP + uridine
show the reaction diagram
-
-
-
-
?
UpU + H2O
5'-UMP + uridine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
RNA + H2O
nucleoside 5'-monophosphates
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
strongly activating
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Physarum polycephalum
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.1
-
RNase Phy II, assay at
4.5
-
RNase Phy II, assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
RNase Phy I and II, assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
submersed culture
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
secretion, both enzyme forms
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24500
-
RNase Phy II, gel filtration
25000
-
RNase Phy I, gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 6
-
very labile below pH 3.0, stable up to pH 6.0
654983
6.5
-
inactivation above this value
134332
8
-
nactivation, RNase Phy I and II
655959
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
pH 4.5 for 12 hours : 55% of activity remains, pH 6.5 for 12 hours : 50% of activity remains, pH 8.5 for 12 hours : 40% of activity remains
20 - 50
-
stable upto 45°C, loss of approximately 30% activity at 50°C, inactive at 55°C
37
-
pH 4.5 for 1 h : 60% of activity remains, pH 4.5 for 12 hours : 20% of activity remains
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
RNase Phy I and II
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE