We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The taxonomic range for the selected organisms is: Escherichia coli The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
exonucleolytic cleavage of oligonucleotides to yield nucleoside 5'-phosphates
Synonyms
rnase t, oligoribonuclease, rexo2, nanornase, cpsorn, small fragment nuclease, oligonucleotidase, xc847,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
nucleotidase, oligo-
-
-
-
-
oligoribonuclease
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of phosphoric ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1,N6-etheno-2'-dApG + H2O
1,N6-etheno-2'-deoxyadenosine + GMP
-
-
-
?
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
dApG + H2O
2'-deoxyadenosine + GMP
-
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
hydrolyzes oligodeoxyribonucleotides and oligoribonucleotides
-
-
?
5'-p-nitrophenyl-TMP + H2O
p-nitrophenol + TMP
-
-
-
-
?
CpC + H2O
5'-CMP + cytidine
-
-
-
-
?
CpU + H2O
3'-CMP + uridine
-
-
-
-
?
DNA + H2O
nucleoside 5'-phosphates
mRNA + H2O
nucleoside 5'-phosphates
-
required for for degradation of mRNA to mononucleotides
-
-
?
NAD+ + H2O
NMN + AMP
-
-
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
RNA + H2O
nucleoside 5'-phosphates
-
active after dissociation from a ribonucleoprotein
-
-
?
UpC + H2O
5'-CMP + 5'-UMP
-
-
-
-
?
UpU + H2O
5'-UMP
-
-
-
-
?
additional information
?
-
DNA + H2O
nucleoside 5'-phosphates
-
no activity
-
-
?
DNA + H2O
nucleoside 5'-phosphates
-
active after dissociation from a ribonucleoprotein
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
-
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
processive degradation
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
however higher affinity to longer chains
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
exonuclease: 3`to 5'-direction
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
reaction rate inversely proportional to chain length of substrate
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
specific for short ribooligonucleotides
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
initial site of attack: 3'-hydroxyl end
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
also hydrolyses NAD+ to NMN and AMP, mechanism
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
essential for complete degradation of mRNA
-
-
?
additional information
?
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
additional information
?
-
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
additional information
?
-
poor activity with 4-nitrophenyl phosphate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5'-phosphoguanylyl-(3',5')-guanosine + H2O
GMP + guanosine
i.e. pGpG
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
additional information
?
-
oligonucleotides + H2O
nucleoside 5'-phosphates
-
-
-
-
?
oligonucleotides + H2O
nucleoside 5'-phosphates
-
essential for complete degradation of mRNA
-
-
?
additional information
?
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
additional information
?
-
-
oligoribonuclease is a common downstream target of lithium-induced pAp accumulation (inhibition of the pAp-degrading enzyme by lithium is widely used to treat bipolar disorders)
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
-
-
Mn2+
-
strong stimulation
Mn2+
-
preferred over Mg2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EDTA
complete inhibition at 50 mM
Ni2+
-
effective competitive inhibitor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.54 - 1.9
5'-p-nitrophenyl-TMP
0.54
5'-p-nitrophenyl-TMP
-
in the presence of 3 mM Mn2+
1.9
5'-p-nitrophenyl-TMP
-
in the presence of 1 mM Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.15 - 2.3
5'-p-nitrophenyl-TMP
2.15
5'-p-nitrophenyl-TMP
-
in the presence of 1 mM Mn2+
2.3
5'-p-nitrophenyl-TMP
-
in the presence of 3 mM Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7 - 9.5
-
pH 7.0: 50% of maximum activity, pH 9.5: 70% of maximum activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
supernatant fraction
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20000
-
2 * 20000, amino acid analysis, SDS-PAGE
21638
-
2 * 21638, electrospray ionisation mass spectrometry
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
tetramer
-
crystallization data
dimer
-
2 * 20000, amino acid analysis, SDS-PAGE
dimer
-
crystals grown in presence of Mn2+, crystallization data
homodimer
crystallography
homodimer
-
2 * 21638, electrospray ionisation mass spectrometry
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
belongs to space group I422, with unit cell parameters a = 213.1, b = 213.1, c = 149.2, 90.0, 90.0, 90.0, to 30.0-3.1 A resolution, opposing dimeric arrangement, with the catalytic DEDD residues from one monomer closely juxtaposed with a large basic patch on the other monomer
both native and selenomethionine-labeled enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
ORN mutant, shows growth defect, YtqI from Bacillus subtilis is able to complement the mutant
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
copurifies with polynucleotide phosphorylase
-
on Ni-NTA column and by gel filtration, more than 95% pure
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI
-
overexpression of the orn gene in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
requirement of dimerization for RNase T action
additional information
-
requirement of dimerization for RNase T action
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Niyogi, S.K.; Datta, A.K.
A novel oligoribonuclease of Escherichia coli. I. Isolation and properties
J. Biol. Chem.
250
7307-7312
1975
Escherichia coli
brenda
Datta, A.K.; Niyogi, S.N.
A novel oligoribonuclease of Escherichia coli. II. Mechanism of action
J. Biol. Chem.
250
7313-7319
1975
Escherichia coli
brenda
Elson,D.
Latent enzymic activity of a ribonucleoprotein isolated from Escherichia coli
Biochim. Biophys. Acta
36
372-386
1959
Escherichia coli
brenda
Yu, D.; Deutscher, M.P.
Oligoribonuclease is distinct from the other known exoribonucleases of Escherichia coli
J. Bacteriol.
177
4137-4139
1995
Escherichia coli
brenda
Zhang, X.; Zhu, L.; Deutscher, M.P.
Oligoribonuclease is encoded by a highly conserved gene in the 3'-5' exonuclease superfamily
J. Bacteriol.
180
2779-2781
1998
Escherichia coli
brenda
Ghosh, S.; Deutscher, M.P.
Oligoribonuclease is an essential component of the mRNA decay pathway
Proc. Natl. Acad. Sci. USA
96
4372-4377
1999
Escherichia coli
brenda
Fiedler, T.J.; Vincent, H.A.; Zuo, Y.; Gavrialov, O.; Malhotra, A.
Purification and crystallization of Escherichia coli oligoribonuclease
Acta Crystallogr. Sect. D
60
736-739
2004
Escherichia coli
brenda
Mechold, U.; Ogryzko, V.; Ngo, S.; Danchin, A.
Oligoribonuclease is a common downstream target of lithium-induced pAp accumulation in Escherichia coli and human cells
Nucleic Acids Res.
34
2364-2373
2006
Escherichia coli (P0A784), Escherichia coli, Homo sapiens
brenda
Mechold, U.; Fang, G.; Ngo, S.; Ogryzko, V.; Danchin, A.
YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity
Nucleic Acids Res.
35
4552-4561
2007
Bacillus subtilis, Escherichia coli
brenda
Young Park, A.; Elvin, C.M.; Hamdan, S.M.; Wood, R.J.; Liyou, N.E.; Hamwood, T.E.; Jennings, P.A.; Dixon, N.E.
Hydrolysis of the 5-p-nitrophenyl ester of TMP by oligoribonucleases (ORN) from Escherichia coli, Mycobacterium smegmatis, and human
Protein Expr. Purif.
57
180-187
2008
Escherichia coli, Homo sapiens, Mycolicibacterium smegmatis
brenda
Zuo, Y.; Zheng, H.; Wang, Y.; Chruszcz, M.; Cymborowski, M.; Skarina, T.; Savchenko, A.; Malhotra, A.; Minor, W.
Crystal structure of RNase T, an exoribonuclease involved in tRNA maturation and end turnover
Structure
15
417-428
2007
Escherichia coli (P30014), Escherichia coli, Pseudomonas aeruginosa (Q9HY82), Pseudomonas aeruginosa
brenda
Zhou, J.; Zheng, Y.; Roembke, B.; Robinson, S.; Opoku-Temeng, C.; Sayre, D.; Sintim, H.
Fluorescent analogs of cyclic and linear dinucleotides as phosphodiesterase and oligoribonuclease activity probes
RSC Adv.
7
5421-5426
2017
Pseudomonas aeruginosa (A0A069Q255), Mycolicibacterium smegmatis (A0A8B4R3D6), Escherichia coli (P0A784)
-
brenda