Information on EC 2.8.2.27 - quercetin-3-sulfate 4'-sulfotransferase

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The expected taxonomic range for this enzyme is: Flaveria chlorifolia

EC NUMBER
COMMENTARY
2.8.2.27
-
RECOMMENDED NAME
GeneOntology No.
quercetin-3-sulfate 4'-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3'-phosphoadenylyl sulfate + quercetin 3-sulfate = adenosine 3',5'-bisphosphate + quercetin 3,4'-bissulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
-
quercetin sulfate biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:quercetin-3-sulfate 4'-sulfotransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
flavonol 4'-sulfotransferase
-
-
-
-
PAPS:flavonol 3-sulfate 4'-sulfotransferase
-
-
-
-
sulfotransferase, flavonol 4'-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
121855-12-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate
isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate
isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
38% of activity compared to quercetin 3-sulfate
-
?
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate
isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
38% of activity compared to quercetin 3-sulfate
-
?
3'-phosphoadenylylsulfate + kaempferol 3-sulfate
kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + kaempferol 3-sulfate
kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
45% of activity compared to quercetin 3-sulfate
-
?
3'-phosphoadenylylsulfate + kaempferol 3-sulfate
kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
45% of activity compared to quercetin 3-sulfate
-
?
3'-phosphoadenylylsulfate + patuletin 3-sulfate
patuletin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
12% of activity compared to quercetin 3-sulfate
-
?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
-
tamarixetin 3-sulfate, flavonol aglycones, or adenosine 5'-phosphosulfate are no substrates
-
-
-
additional information
?
-
-
involved in biosynthesis of polysulfated flavonoids
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
-
involved in biosynthesis of polysulfated flavonoids
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
no requirement for divalent cations
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Phosphate buffer
-
complete inhibition
-
additional information
-
EDTA up to 10 mM, SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide, all at 1 and 10 mM, are not inhibitory
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00038
-
3'-phosphoadenylylsulfate
-
-
0.00038
-
3'-phosphoadenylylsulfate
-
30C, pH 7.5
0.00036
-
quercetin 3-sulfate
-
-
0.00036
-
quercetin 3-sulfate
-
30C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.002
-
-
30C, pH 7.5
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35000
-
-
gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, bovine serum albumin up to 1 mg/ml, half-life of 3 days
-
4C, half-life of 24 h
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
construction of chimeric enzymes with EC2.8.2.25 in order to define domains responsible for substrate binding and position