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Literature summary for 2.8.2.27 extracted from

  • Varin, L.; Ibrahim, R.K.
    Partial purification and characterization of three flavonol-specific sulfotransferases from Flaveria chloraefolia (1989), Plant Physiol., 90, 977-981.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.27

Inhibitors

Inhibitors Comment Organism Structure
additional information EDTA up to 10 mM, SH-group reagents, e.g. p-chloromercuribenzoate, iodoacetate, iodoacetamide, all at 1 and 10 mM, are not inhibitory Flaveria chlorifolia
phosphate buffer complete inhibition Flaveria chlorifolia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00036
-
 quercetin 3-sulfate 30°C, pH 7.5 Flaveria chlorifolia
0.00038
-
 3'-phosphoadenylylsulfate 30°C, pH 7.5 Flaveria chlorifolia

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Flaveria chlorifolia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Flaveria chlorifolia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + quercetin 3-sulfate Flaveria chlorifolia
-
 quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Flaveria chlorifolia
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 30°C, pH 7.5 Flaveria chlorifolia

Storage Stability

Storage Stability Organism
-20°C, bovine serum albumin up to 1 mg/ml, half-life of 3 days Flaveria chlorifolia
4°C, half-life of 24 h Flaveria chlorifolia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + isorhamnetin 3-sulfate 38% of activity compared to quercetin 3-sulfate Flaveria chlorifolia isorhamnetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
3'-phosphoadenylylsulfate + kaempferol 3-sulfate 45% of activity compared to quercetin 3-sulfate Flaveria chlorifolia kaempferol 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
3'-phosphoadenylylsulfate + patuletin 3-sulfate 12% of activity compared to quercetin 3-sulfate Flaveria chlorifolia patuletin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
3'-phosphoadenylylsulfate + quercetin 3-sulfate
-
 Flaveria chlorifolia quercetin 3,4'-disulfate + adenosine 3',5'-bisphosphate
-
 ?
additional information tamarixetin 3-sulfate, flavonol aglycones, or adenosine 5'-phosphosulfate are no substrates Flaveria chlorifolia ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Flaveria chlorifolia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Flaveria chlorifolia