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Information on EC 2.7.7.60 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession P69834

for references in articles please use BRENDA:EC2.7.7.60
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EC Tree
IUBMB Comments
The enzyme from Escherichia coli requires Mg2+ or Mn2+. ATP or UTP can replace CTP, but both are less effective. GTP and TTP are not substrates. Forms part of an alternative nonmevalonate pathway for terpenoid biosynthesis (for diagram, click here).
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: P69834
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
cytidylyltransferase, pfispd, 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase, aamct, mtispd, mep cytidylyltransferase, 2-c-methyl-d-erythritol-4-phosphate cytidyltransferase, 4-diphosphocytidyl-2c-methyl-d-erythritol synthase, cdp-me synthetase, pvispd, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-C-methyl erythritol 4-phosphate cytidylyltransferase
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2-C-methyl-D-erythritol-4-phosphate cytidylyltransferase
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2-C-methylerythritol 4-cytidylyltransferase
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4-diphosphocytidyl-2-C-methyl-D-erythritol synthetase
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4-diphosphocytidyl-2-C-methylerythritol synthase
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4-diphosphocytidyl-2C-methyl-D-erythritol synthase
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CDP-ME synthetase
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cytidylyltransferase, 2-C-methylerythritol 4-
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MCT
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MEP cytidylyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
CTP:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
The enzyme from Escherichia coli requires Mg2+ or Mn2+. ATP or UTP can replace CTP, but both are less effective. GTP and TTP are not substrates. Forms part of an alternative nonmevalonate pathway for terpenoid biosynthesis (for diagram, click here).
CAS REGISTRY NUMBER
COMMENTARY hide
251990-59-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + 2-C-methyl-D-erythritol 4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
show the reaction diagram
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ATP, UTP, GTP, ITP, dTTP, dGTP, dCTP ann dATP tested as substrates besides CTP
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r
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
show the reaction diagram
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + 2-C-methyl-D-erythritol-4-phosphate
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
show the reaction diagram
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-
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
26
CTP
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per subunit
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33
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cell extract
67
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pure protein
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ISPD_ARATH
302
0
33937
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25472
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x * 29000, SDS-PAGE, x * 25472 is predicted from sequence of c DNA
29000
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x * 29000, SDS-PAGE, x * 25472 is predicted from sequence of c DNA
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 29000, SDS-PAGE, x * 25472 is predicted from sequence of c DNA
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic domain of recombinant enzyme, structure contains a cytidine monophosphate bound in the active site suggesting a mechanism for feedback regulation of enzyme activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Source 15 Q and Superdex 200 column
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli M15(pREP4)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rohdich, F.; Wungsintaweekul, J.; Eisenreich, W.; Richter, G.; Schuhr, C.A.; Hecht, S.; Zenk, M.H.; Bacher, A.
Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
97
6451-6456
2000
Arabidopsis thaliana, Escherichia coli (Q46893), Escherichia coli
Manually annotated by BRENDA team
Rohdich, F.; Wungsintaweekul, J.; Fellermeier, M.; Sagner, S.; Herz, S.; Kis, K.; Eisenreich, W.; Bacher, A.; Zenk, M.H.
Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol
Proc. Natl. Acad. Sci. USA
96
11758-11763
1999
Arabidopsis thaliana, Escherichia coli, Escherichia coli DH5-alpha
Manually annotated by BRENDA team
Gabrielsen, M.; Kaiser, J.; Rohdich, F.; Eisenreich, W.; Laupitz, R.; Bacher, A.; Bond, C.S.; Hunter, W.N.
The crystal structure of a plant 2C-methyl-D-erythritol 4-phosphate cytidylyltransferase exhibits a distinct quaternary structure compared to bacterial homologues and a possible role in feedback regulation for cytidine monophosphate
FEBS J.
273
1065-1073
2006
Arabidopsis thaliana (P69834), Arabidopsis thaliana
Manually annotated by BRENDA team