Literature summary for 2.7.7.60 extracted from

Rohdich, F.; Wungsintaweekul, J.; Eisenreich, W.; Richter, G.; Schuhr, C.A.; Hecht, S.; Zenk, M.H.; Bacher, A.
Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana (2000), Proc. Natl. Acad. Sci. USA, 97, 6451-6456.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli M15(pREP4)	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	CTP	-	Escherichia coli
0.114	-	CTP	-	Arabidopsis thaliana
0.131	-	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	Escherichia coli
0.5	-	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
plastid	-	Arabidopsis thaliana	9536	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Escherichia coli
Mg2+	absolute requirement of divalent metal ions like Ni2+, Mn2+, Co2+, Ca2+, Cu2+ and Zn2+ for catalytic acitvity, maximum activity with Mg2+	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25472	-	x * 29000, SDS-PAGE, x * 25472 is predicted from sequence of c DNA	Arabidopsis thaliana
25737	-	x * 25737 is predicted from sequence of c DNA	Escherichia coli
29000	-	x * 29000, SDS-PAGE, x * 25472 is predicted from sequence of c DNA	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-
Escherichia coli	Q46893	-	-

Purification (Commentary)

Purification (Comment)	Organism
Source 15 Q and Superdex 200 column	Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
33	-	cell extract	Arabidopsis thaliana
67	-	pure protein	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CTP + 2-C-methyl-D-erythritol 4-phosphate	-	Escherichia coli	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	r
CTP + 2-C-methyl-D-erythritol 4-phosphate	ATP, UTP, GTP, ITP, dTTP, dGTP, dCTP ann dATP tested as substrates besides CTP	Arabidopsis thaliana	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	r

Subunits

Subunits	Comment	Organism
?	x * 29000, SDS-PAGE, x * 25472 is predicted from sequence of c DNA	Arabidopsis thaliana
?	x * 25737 is predicted from sequence of c DNA	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Escherichia coli
37	-	assay at	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9	-	CTP	per subunit	Escherichia coli
26	-	CTP	per subunit	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Arabidopsis thaliana
8	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)