Information on EC 2.7.6.5 - GTP diphosphokinase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
2.7.6.5
-
RECOMMENDED NAME
GeneOntology No.
GTP diphosphokinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
GDP can also act as acceptor
-
-
-
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
show the reaction diagram
bifunctional enzyme, additionally has polynucleotide phosphorylase activities
Q53597, -
PATHWAY
KEGG Link
MetaCyc Link
ppGpp biosynthesis
-
Purine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:GTP 3'-diphosphotransferase
GDP can also act as acceptor.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(p)ppGpp synthetase
-
-
(p)ppGpp synthetase
-
-
(p)ppGpp synthetase
Mycobacterium smegmatis mc2155
-
-
-
(p)ppGpp synthetase I
-
-
-
-
(p)ppGpp synthetase II
-
-
-
-
(p)ppGpp synthetase/hydrolase
-
-
ATP-GTP 3'-diphosphotransferase
-
-
-
-
ATP:GTP pyrophosphoryl transferase
-
-
ATP:GTP pyrophosphoryl transferase
Escherichia coli MRE 600
-
-
-
GPSI
-
-
-
-
GPSII
-
-
-
-
GTP pyrophosphokinase
-
-
-
-
GTP pyrophosphokinase
-
-
guanosine 3',5'-polyphosphate synthase
-
-
-
-
guanosine 3',5'-polyphosphate synthetase
-
-
-
-
guanosine 5',3'-polyphosphate synthetase
-
-
-
-
guanosine pentaphosphate synthetase
-
-
-
-
Rel
Mycobacterium smegmatis mc2155
-
-
-
Rel/Spo protein
-
-
RelMtb protein
-
-
SF
Escherichia coli MRE 600
-
-
-
stringent factor
-
-
-
-
stringent factor
-
-
stringent factor
Escherichia coli MRE 600
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
63690-89-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
MRE 600
-
-
Manually annotated by BRENDA team
Escherichia coli MRE 600
MRE 600
-
-
Manually annotated by BRENDA team
2 different enzymes: (p)ppGpp synthetase I, (p)ppGpp synthetase II
-
-
Manually annotated by BRENDA team
Mycobacterium smegmatis mc2155
-
-
-
Manually annotated by BRENDA team
subsp. equisimilis
-
-
Manually annotated by BRENDA team
ATCC 21828
-
-
Manually annotated by BRENDA team
2 enzyme forms: GPS I
-
-
Manually annotated by BRENDA team
2 enzyme forms: GPS I; GPS II
-
-
Manually annotated by BRENDA team
ATCC 11523
-
-
Manually annotated by BRENDA team
Streptomyces coelicolor A3
A3
-
-
Manually annotated by BRENDA team
Streptomyces fradiae UC8306
UC8306
-
-
Manually annotated by BRENDA team
Streptomyces glaucescens ETH 22794
ETH 22794
-
-
Manually annotated by BRENDA team
ATCC 12434
-
-
Manually annotated by BRENDA team
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
dCDP
Q53597, -
slightly stimulates synthesis of guanosine 3'-diphosphate 5'-triphosphate, inhibits polynucleotide phosphorylase activities of enzyme
ethanol
-
little activity unless activated either by a complex of 70S ribosomes, mRNA and uncharged tRNA or by a solvent like ethanol at approximately 20%
mRNA
-
(p)ppGpp synthetase II does not
mRNA
-
little activity unless activated either by a complex of 70S ribosomes, mRNA and uncharged tRNA or by a solvent like ethanol at approximately 20%
mRNA
-
synthetic, e.g. poly(U), stimulates, level of stimulation is greater in presence of RNA and poly(U) together than with either RNA alone, no activation by ribosomes
tRNA
-
uncharged or charged, stimulation, level of stimulation is greater in presence of RNA and poly(U) together than with either RNA alone
Trypsin
-
incubation with low levels of trypsin activates
-
unacylated tRNA
-
in the ribosomal amino-acyl site (A-site)
-
methanol
-
maximal stimulation of GPSI by 20% v/v
additional information
-
(p)ppGpp synthetase II does not
-
additional information
-
little activity unless activated either by a complex of 70S ribosomes, mRNA and uncharged tRNA or by a solvent like ethanol at approximately 20%
-
additional information
-
GPS I can be activated by incubation with crude mycelial extract, activation is partially inhibited by the inclusion of trypsin inhibitor in reaction mixture; no activation by ribosomes
-
additional information
-
addition of template, unacylated tRNA and ribosomes to the activity assay stimulates SF 30fold when using optimal conditions, activity of SF increases threefold in the presence of twice salt-washed tight-couple ribosomes and twofold in the presence of reassociated ribosomes compared to the endogenous activity of the enzyme
-
additional information
-
ribosome complexes formed with tight binding tRNAVal stimulate enzyme activity at lower concentrations than that required for ribosome complexes formed with the weaker binding tRNAPhe
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the crystallographic asymmetric unit contains two copies of RelSeq 1-385, two catalytic domains are clearly evident within each monomer, with the hydrolase (residues 5-159) and the synthetase (residues 176-371) domains joined by an overlapping central 3-helix bundle (residues 135-195)
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the purified SF is stored in the freezer, at a concentration of 1.0 mg/ml in 20% (v/v) glycerol, without forming a precipitate or loss of activity
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
drug development
-
the structural knowledge of the self-regulatory mechanisms for controlling the opposing catalytic activities of RelSeq can be used to design specific inhibitors that interfere with either of the active sites and may have the potential of being developped into powerful antibacterial drugs