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Literature summary for 2.7.6.5 extracted from

  • Murdeshwar, M.S.; Chatterji, D.
    MS_RHII-RSD, a dual-function RNase HII-(p)ppGpp synthetase from Mycobacterium smegmatis (2012), J. Bacteriol., 194, 4003-4014.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.6.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ the enzyme shows a marked decrease in guanosine tetraphosphate synthesis activity with increasing Mg2+ ion concentration Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.163
-
 GTP in 50 mM HEPES, pH 7.5, at 37°C Mycolicibacterium smegmatis
1.532
-
 GDP in 50 mM HEPES, pH 7.5, at 37°C Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme exploits a single Mg2+ ion to catalyze guanosine 3'-diphosphate 5'-triphosphate synthesis Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, and gel filtration Mycolicibacterium smegmatis

Renatured (Commentary)

Renatured (Comment) Organism
the purified enzyme is renatured by stepwise dialysis in buffers containing sequentially decreasing concentrations of urea (50mM Tris-HCl, pH 7.9 at 4°C, 150 mM NaCl, 100 mM imidazole, and 4 M, 2 M, and 0 M urea, in that order) Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GDP the enzyme utilizes GDP less efficiently than GTP Mycolicibacterium smegmatis AMP + guanosine 3',5'-bis-diphosphate
-
 ?
ATP + GDP the enzyme utilizes GDP less efficiently than GTP Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 AMP + guanosine 3',5'-bis-diphosphate
-
 ?
ATP + GTP the enzyme uses GTP approximately twice as well as GDP Mycolicibacterium smegmatis AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
ATP + GTP the enzyme uses GTP approximately twice as well as GDP Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
additional information the enzyme also possesses a potent Mn2+-dependent guanosine tetraphosphate hydrolysis activity, with complete hydrolysis to GDP and diphosphate Mycolicibacterium smegmatis ?
-
 ?
additional information the enzyme also possesses a potent Mn2+-dependent guanosine tetraphosphate hydrolysis activity, with complete hydrolysis to GDP and diphosphate Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?

Synonyms

Synonyms Comment Organism
(p)ppGpp synthetase
-
 Mycolicibacterium smegmatis
Rel
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73
-
 GDP in 50 mM HEPES, pH 7.5, at 37°C Mycolicibacterium smegmatis
127
-
 GTP in 50 mM HEPES, pH 7.5, at 37°C Mycolicibacterium smegmatis

pI Value

Organism Comment pI Value Maximum pI Value
Mycolicibacterium smegmatis calculated from amino acid sequence
-
 8.1