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Information on EC 2.7.4.6 - nucleoside-diphosphate kinase and Organism(s) Pyrobaculum aerophilum and UniProt Accession Q8ZWY4

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IUBMB Comments
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
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This record set is specific for:
Pyrobaculum aerophilum
UNIPROT: Q8ZWY4
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Word Map
The taxonomic range for the selected organisms is: Pyrobaculum aerophilum
The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
kinase, nucleoside diphosphate (phosphorylating)
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NDK
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nucleoside 5'-diphosphate kinase
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nucleoside diphosphate (UDP) kinase
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nucleoside diphosphokinase
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nucleotide phosphate kinase
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UDP kinase
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uridine diphosphate kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-51-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dATP + ADP
dADP + ATP
show the reaction diagram
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
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dCTP + ADP
dCDP + ATP
show the reaction diagram
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
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dGTP + ADP
dGDP + ATP
show the reaction diagram
the wild-type enzyme is not active when using dGTP as the phosphate donor. The Pyrobaculum aerophilum NDP kinase sequence contains two unique segments not present in other NDP kinases, comprising residues 66–100 and 156–165. Deletion mutants of the NDP kinase lacking either or both of these inserts have an altered substrate specificity, allowing dGTP as the phosphate donor
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dTTP + ADP
dTDP + ATP
show the reaction diagram
at 25°C, the wild-type enzyme has the highest affinity for dCTP, followed by dATP, and then dTTP. dCTP is the best phosphate donor at both 25°C and 50°C
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method at room temperature
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pdelacq, J.D.; Waldo, G.S.; Cabantous, S.; Liong, E.C.; Terwilliger, T.C.
Structural and functional features of an NDP kinase from the hyperthermophile crenarchaeon Pyrobaculum aerophilum
Protein Sci.
14
2562-2573
2005
Pyrobaculum aerophilum (Q8ZWY4), Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523 (Q8ZWY4)
Manually annotated by BRENDA team