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Information on EC 2.7.4.22 - UMP kinase and Organism(s) Helicobacter pylori and UniProt Accession P56106

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EC Tree
IUBMB Comments
This enzyme is strictly specific for UMP as substrate and is used by prokaryotes in the de novo synthesis of pyrimidines, in contrast to eukaryotes, which use the dual-specificity enzyme UMP/CMP kinase (EC 2.7.4.14) for the same purpose . This enzyme is the subject of feedback regulation, being inhibited by UTP and activated by GTP .
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This record set is specific for:
Helicobacter pylori
UNIPROT: P56106
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The taxonomic range for the selected organisms is: Helicobacter pylori
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
hide
ATP
+
UMP
=
ADP
+
UDP
+
=
+
Synonyms
umpk, ump kinase, uridine monophosphate kinase, uridylate kinase, ump-kinase, umpks, ssumpk, xc1936, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
HP0777 protein
-
uridine monophosphate kinase
-
uridylate kinase
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:UMP phosphotransferase
This enzyme is strictly specific for UMP as substrate and is used by prokaryotes in the de novo synthesis of pyrimidines, in contrast to eukaryotes, which use the dual-specificity enzyme UMP/CMP kinase (EC 2.7.4.14) for the same purpose [2]. This enzyme is the subject of feedback regulation, being inhibited by UTP and activated by GTP [1].
CAS REGISTRY NUMBER
COMMENTARY hide
9036-23-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + UMP
ADP + UDP
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
100% activity at 2 mM
Mn2+
the enzyme prefers Mn2+ over Mg2+
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
UTP
43% inhibition at 0.38 mM, 51.2% inhibition at 0.53 mM. About 21% of UMP kinase activity remains at 0.94-1.33 mM of UTP
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GTP
activates at 0.125 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.304 - 0.4
ATP
0.054 - 0.125
UMP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
189.6 - 436.2
ATP
117.9 - 545.2
UMP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
623.1 - 1250
ATP
2181 - 4362
UMP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45
UTP
Helicobacter pylori
at pH 7.4 and 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
when Mn2+ serves as the cofactor, maximal enzyme activity is found at pH 7.4
9
when Mg2+ serves as the cofactor, maximal enzyme activity is found at pH 9.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27500
x * 27500, His6-tagged enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 27500, His6-tagged enzyme, SDS-PAGE
hexamer
x-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complexed with GTP, hanging drop vapor diffusion method, using 150 mM sodium acetate pH 4.8, 270 mM ammonium sulfate, 18% (w/v) PEG MME 550. Complexed with UDP, hanging drop vapor diffusion method, using 0.15 M sodium acetate pH 4.8, 0.35 M ammonium sulfate, 28% (w/v)PEG MME 550
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
the UMP kinase activity of the HP0777 protein is lost within a week when preserved at pH 9.0, but can be maintained for at least 2 weeks in 50 mM Tris-HCl, pH 7.4
721458
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain SG13009
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chu, C.H.; Liu, M.H.; Chen, P.C.; Lin, M.H.; Li, Y.C.; Hsiao, C.D.; Sun, Y.J.
Structures of Helicobacter pylori uridylate kinase: insight into release of the product UDP
Acta Crystallogr. Sect. D
68
773-783
2012
Helicobacter pylori (P56106), Helicobacter pylori
Manually annotated by BRENDA team
Lee, M.J.; Chien-Liang, L.; Tsai, J.Y.; Sue, W.T.; Hsia, W.S.; Huang, H.
Identification and biochemical characterization of a unique Mn2+-dependent UMP kinase from Helicobacter pylori
Arch. Microbiol.
192
739-746
2010
Helicobacter pylori (P56106), Helicobacter pylori
Manually annotated by BRENDA team