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Literature summary for 2.7.4.22 extracted from

  • Lee, M.J.; Chien-Liang, L.; Tsai, J.Y.; Sue, W.T.; Hsia, W.S.; Huang, H.
    Identification and biochemical characterization of a unique Mn2+-dependent UMP kinase from Helicobacter pylori (2010), Arch. Microbiol., 192, 739-746.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GTP activates at 0.125 mM Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain SG13009 Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
UTP 43% inhibition at 0.38 mM, 51.2% inhibition at 0.53 mM. About 21% of UMP kinase activity remains at 0.94-1.33 mM of UTP Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
UMP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
0.125
-
UMP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori
0.304
-
ATP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
0.4
-
ATP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 100% activity at 2 mM Helicobacter pylori
Mn2+ the enzyme prefers Mn2+ over Mg2+ Helicobacter pylori
additional information Co2+ and Ni2+ substitute poorly for Mg2+ and give limited enzyme activity, while Ca2+ and Zn2+ are unable to activate UMP kinase within 0-5 mM. UMP kinase activity of the HP0777 protein is completely abolished in the absence of divalent cations Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
x * 27500, His6-tagged enzyme, SDS-PAGE Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori P56106
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UMP
-
Helicobacter pylori ADP + UDP
-
?

Subunits

Subunits Comment Organism
? x * 27500, His6-tagged enzyme, SDS-PAGE Helicobacter pylori

Synonyms

Synonyms Comment Organism
HP0777 protein
-
Helicobacter pylori
uridine monophosphate kinase
-
Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
117.9
-
UMP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
189.6
-
ATP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
436.2
-
ATP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori
545.2
-
UMP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
when Mn2+ serves as the cofactor, maximal enzyme activity is found at pH 7.4 Helicobacter pylori
9
-
when Mg2+ serves as the cofactor, maximal enzyme activity is found at pH 9.0 Helicobacter pylori

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
the UMP kinase activity of the HP0777 protein is lost within a week when preserved at pH 9.0, but can be maintained for at least 2 weeks in 50 mM Tris-HCl, pH 7.4 Helicobacter pylori

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.45
-
at pH 7.4 and 30°C Helicobacter pylori UTP

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
623.1
-
ATP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
1250
-
ATP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori
2181
-
UMP in the presence of 2 mM Mg2+, at pH 7.4 and 30°C Helicobacter pylori
4362
-
UMP in the presence of 2 mM Mn2+, at pH 7.4 and 30°C Helicobacter pylori