Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | activates at 0.125 mM | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain SG13009 | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UTP | 43% inhibition at 0.38 mM, 51.2% inhibition at 0.53 mM. About 21% of UMP kinase activity remains at 0.94-1.33 mM of UTP | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
UMP | in the presence of 2 mM Mg2+, at pH 7.4 and 30°C | Helicobacter pylori | |
0.125 | - |
UMP | in the presence of 2 mM Mn2+, at pH 7.4 and 30°C | Helicobacter pylori | |
0.304 | - |
ATP | in the presence of 2 mM Mg2+, at pH 7.4 and 30°C | Helicobacter pylori | |
0.4 | - |
ATP | in the presence of 2 mM Mn2+, at pH 7.4 and 30°C | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 100% activity at 2 mM | Helicobacter pylori | |
Mn2+ | the enzyme prefers Mn2+ over Mg2+ | Helicobacter pylori | |
additional information | Co2+ and Ni2+ substitute poorly for Mg2+ and give limited enzyme activity, while Ca2+ and Zn2+ are unable to activate UMP kinase within 0-5 mM. UMP kinase activity of the HP0777 protein is completely abolished in the absence of divalent cations | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
x * 27500, His6-tagged enzyme, SDS-PAGE | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | P56106 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Helicobacter pylori | ADP + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27500, His6-tagged enzyme, SDS-PAGE | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
HP0777 protein | - |
Helicobacter pylori |
uridine monophosphate kinase | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
117.9 | - |
UMP | in the presence of 2 mM Mg2+, at pH 7.4 and 30°C | Helicobacter pylori | |
189.6 | - |
ATP | in the presence of 2 mM Mg2+, at pH 7.4 and 30°C | Helicobacter pylori | |
436.2 | - |
ATP | in the presence of 2 mM Mn2+, at pH 7.4 and 30°C | Helicobacter pylori | |
545.2 | - |
UMP | in the presence of 2 mM Mn2+, at pH 7.4 and 30°C | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
when Mn2+ serves as the cofactor, maximal enzyme activity is found at pH 7.4 | Helicobacter pylori |
9 | - |
when Mg2+ serves as the cofactor, maximal enzyme activity is found at pH 9.0 | Helicobacter pylori |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
the UMP kinase activity of the HP0777 protein is lost within a week when preserved at pH 9.0, but can be maintained for at least 2 weeks in 50 mM Tris-HCl, pH 7.4 | Helicobacter pylori |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.45 | - |
at pH 7.4 and 30°C | Helicobacter pylori | UTP |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
623.1 | - |
ATP | in the presence of 2 mM Mg2+, at pH 7.4 and 30°C | Helicobacter pylori | |
1250 | - |
ATP | in the presence of 2 mM Mn2+, at pH 7.4 and 30°C | Helicobacter pylori | |
2181 | - |
UMP | in the presence of 2 mM Mg2+, at pH 7.4 and 30°C | Helicobacter pylori | |
4362 | - |
UMP | in the presence of 2 mM Mn2+, at pH 7.4 and 30°C | Helicobacter pylori |