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Information on EC 2.7.1.36 - mevalonate kinase and Organism(s) Methanocaldococcus jannaschii and UniProt Accession Q58487

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EC Tree
IUBMB Comments
CTP, GTP and UTP can also act as donors.
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This record set is specific for:
Methanocaldococcus jannaschii
UNIPROT: Q58487
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Word Map
The taxonomic range for the selected organisms is: Methanocaldococcus jannaschii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mevalonate kinase, mvk, mva kinase, tcmvk, temvk, mevalonic kinase, mevalonate 5-phosphotransferase, atp:mevalonate 5-phosphotransferase, mevalonic acid kinase, atp mevalonate 5-phosphotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP:mevalonate 5-phosphotransferase
-
-
-
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kinase, mevalonate (phosphorylating)
-
-
-
-
mevalonate 5-phosphotransferase
-
-
-
-
mevalonate phosphokinase
-
-
-
-
mevalonic acid kinase
-
-
-
-
mevalonic kinase
-
-
-
-
MVA kinase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-mevalonate 5-phosphotransferase
CTP, GTP and UTP can also act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-52-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-mevalonate
ADP + (R)-5-phosphomevalonate
show the reaction diagram
-
-
-
?
ATP + (R)-mevalonate
ADP + (R)-5-phosphomevalonate
show the reaction diagram
-
-
-
-
?
ATP + mevalonate
ADP + phosphomevalonate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + mevalonate
ADP + phosphomevalonate
show the reaction diagram
-
involved in mevalonate pathway of terpenoid biosynthesis
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
farnesyl diphosphate
-
0.01 mM, 65% inhibition
geranyl diphosphate
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0.01 mM, 55% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.061 - 0.111
(R)-mevalonate
0.069
(R,S)-mevalonate
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pH 8.0, 70°C, recombinant mevalonate kinase
0.069
(RS)mevalonate
-
-
0.092 - 3.36
ATP
0.11 - 9.28
mevalonate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1 - 28.5
(R)-mevalonate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
387
-
recombinant mevalonate kinase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
-
at least 60% of maximal activity between pH 5.0 and 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34
-
wild-type, 25% of maximal activity
37 - 90
-
approx. 25% of maximal activity at 40°C and 90°C, respectively
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
MonoP chromatography
8.4
-
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
2 * 37000, SDS-PAGE
68000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, the crystal structure is determined at 2.4 A
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C107A
-
decrease in temperature stability, slight increase in Km value for ATP
C107S
-
decrease in temperature stability, slight increase in Km value for ATP
C107S/C281S
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decrease in temperature stability, slight increase in Km value for ATP
C197A/C281A
-
decrease in temperature stability, slight increase in Km value for ATP
C281A
-
decrease in temperature stability, slight increase in Km value for ATP
C281S
-
decrease in temperature stability, slight increase in Km value for ATP
K272A
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18% of wild-type activity
K272R
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18% of wild-type activity
R196L
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5% of wild-type activity
R196V
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60% of wild-type activity
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
50% loss of activity after 5 min
70
-
no loss of activity after 24 h
83
-
10 min, residual activity of wild-type 50%, mutants C107S and C281S 20%, mutant C107S/C281S 15%, mutants C107A and C281A 20%, mutant C107A/C281A 18%
90
-
50% loss of activity after 15 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant mevalonate kinase, 70°C heat treatment, Talon metal-affinity resin
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, K.X.; Scott, A.I.; Bennett, G.N.
Overexpression, purification, and characterization of the thermostable mevalonate kinase from Methanococcus jannaschii
Protein Expr. Purif.
17
33-40
1999
Methanocaldococcus jannaschii
Manually annotated by BRENDA team
Houten, S.M.; Wanders, R.J.; Waterham, H.R.
Biochemical and genetic aspects of mevalonate kinase and its deficiency
Biochim. Biophys. Acta
1529
19-32
2000
Methanocaldococcus jannaschii, Sus scrofa, Rattus norvegicus (P17256), Homo sapiens (Q03426), Homo sapiens
Manually annotated by BRENDA team
Chu, X.; Liu, X.; Yau, M.; Leung, Y.C.; Li, D.
Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii
Protein Expr. Purif.
30
210-218
2003
Methanocaldococcus jannaschii
Manually annotated by BRENDA team
Chu, X.; Yu, W.; Wu, L.; Liu, X.; Li, N.; Li, D.
Effect of a disulfide bond on mevalonate kinase
Biochim. Biophys. Acta
1774
1571-1581
2007
Methanocaldococcus jannaschii, Rattus norvegicus
Manually annotated by BRENDA team
Yang, D.; Shipman, L.W.; Roessner, C.A.; Scott, A.I.; Sacchettini, J.C.
Structure of the Methanococcus jannaschii mevalonate kinase, a member of the GHMP kinase superfamily
J. Biol. Chem.
277
9462-9467
2002
Methanocaldococcus jannaschii (Q58487), Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661 (Q58487)
Manually annotated by BRENDA team