Literature summary for 2.7.1.36 extracted from

Chu, X.; Liu, X.; Yau, M.; Leung, Y.C.; Li, D.
Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii (2003), Protein Expr. Purif., 30, 210-218.

Search for more literature for 2.7.1.36

Protein Variants

Protein Variants	Comment	Organism
K272A	18% of wild-type activity	Methanocaldococcus jannaschii
K272R	18% of wild-type activity	Methanocaldococcus jannaschii
R196L	5% of wild-type activity	Methanocaldococcus jannaschii
R196V	60% of wild-type activity	Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	mevalonate	pH 7.5, 34°C	Methanocaldococcus jannaschii
0.25	-	mevalonate	pH 7.5, 34°C, K272R mutant mevalonate kinase	Methanocaldococcus jannaschii
0.51	-	mevalonate	pH 7.5, 34°C, K272A mutant mevalonate kinase	Methanocaldococcus jannaschii
1.18	-	ATP	pH 7.5, 34°C	Methanocaldococcus jannaschii
1.22	-	ATP	pH 7.5, 34°C, K272R mutant mevalonate kinase	Methanocaldococcus jannaschii
1.43	-	mevalonate	pH 7.5, 34°C, R196L mutant mevalonate kinase	Methanocaldococcus jannaschii
1.54	-	ATP	pH 7.5, 34°C, R196L mutant mevalonate kinase	Methanocaldococcus jannaschii
2.95	-	ATP	pH 7.5, 34°C, K272A mutant mevalonate kinase	Methanocaldococcus jannaschii
2.98	-	ATP	pH 7.5, 34°C, R196Q mutant mevalonate kinase	Methanocaldococcus jannaschii
3	-	ATP	pH 7.5, 34°C, R196V mutant mevalonate kinase	Methanocaldococcus jannaschii
4.03	-	mevalonate	pH 7.5, 34°C, R196V mutant mevalonate kinase	Methanocaldococcus jannaschii
9.28	-	mevalonate	pH 7.5, 34°C, R196Q mutant mevalonate kinase	Methanocaldococcus jannaschii

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + mevalonate	-	Methanocaldococcus jannaschii	ADP + phosphomevalonate	-	?

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Release 2023.1 (January 2023)