Information on EC 2.6.1.15 - glutamine-pyruvate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.6.1.15
-
RECOMMENDED NAME
GeneOntology No.
glutamine-pyruvate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-glutamine + pyruvate = 2-oxoglutaramate + L-alanine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamine:pyruvate aminotransferase
A pyridoxal-phosphate protein. L-Methionine can act as donor; glyoxylate can act as acceptor.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminotransferase, glutamine-keto acid
-
-
-
-
gamma-glutaminyltransferase
-
-
-
-
glutaminase II
-
-
-
-
glutamine transaminase
-
-
-
-
glutamine transaminase L
-
-
-
-
glutamine-alpha-keto acid transamidase
-
-
-
-
glutamine-alpha-keto acid transaminase
-
-
-
-
glutamine-keto acid aminotransferase
-
-
-
-
glutamine-oxo acid aminotransferase
-
-
-
-
glutamine-oxo-acid transaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-44-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
bifunctional kynurenine aminotransferase/glutamine transaminase, 4 isoforms, expression in COS1-cells
-
-
Manually annotated by BRENDA team
male Sprague-Dawley
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutaramate + glycine
L-glutamine + glyoxylate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-alanine
L-glutamine + pyruvate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-asparagine
L-glutamine + 2-oxosuccinamate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-cysteine
L-glutamine + 3-mercapto-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-ethionine
L-glutamine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-glutamic acid 4-benzylester
L-glutamine + 2-oxopentane-1,5-dioic acid benzyl ester
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-homoserine
L-glutamine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-methionine
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-methionine
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
best amino group donor
-
?
2-oxoglutaramate + L-methionine (SR)-sulfoxide
L-glutamine + 4-methylsulfinyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-methionine sulfone
L-glutamine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-phenylalanine
L-glutamine + phenylpyruvate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-serine
L-glutamine + 3-hydroxy-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
gamma-glutamylhydrazones of 2-oxo acid
L-amino acid + 2-hydroxy-tetrahydro-6-pyridazinone-3-carboxylic acid
show the reaction diagram
-
-
-
?
gamma-glutamylhydrazones of 2-oxo acid
L-amino acid + 2-hydroxy-tetrahydro-6-pyridazinone-3-carboxylic acid
show the reaction diagram
-
gamma-glutamylhydrazones of 4-ethylsulfanyl-2-oxobutanoate, glyoxylate, 2-oxoisovalerate, transfers the 2-amino group of the glutamyl moiety to the oxo acid moiety, the reaction rates are higher than those of the corresponding glutamine alpha-keto acid transaminations
-
?
glyoxylate + 4-glutamylmethylamide
glycine + ?
show the reaction diagram
-
-
-
?
glyoxylate + 4-glutamylmethylamide
glycine + ?
show the reaction diagram
-
-
-
?
glyoxylate + DL-homocysteine
glycine + 2-oxo-4-thiobutanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + glutamic acid 4-methyl ester
glycine + 2-oxoglutarate 4-methyl ester
show the reaction diagram
-
-
-
?
glyoxylate + glutamic acid 4-methyl ester
glycine + 2-oxoglutarate 4-methyl ester
show the reaction diagram
-
-
-
?
glyoxylate + L-2-amino-4-oxo-5-chloropentanoic acid
glycine + 5-chloro-2,4-dioxopentanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-alanine
glycine + pyruvate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-asparagine
glycine + 2-oxosuccinamate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-cysteine
glycine + 3-mercapto-2-oxopropanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-cysteine
glycine + 3-mercapto-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-ethionine
glycine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-ethionine
glycine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
?
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
-
-
-
?
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
-
-
-
?
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
-
-
-
?
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
-
-
2-oxoglutaramate cyclizes spontaneously to form 2-pyrrolidone-5-hydroxy-5-carboxylate
?
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
-
best substrate
-
?
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
glyoxylate + L-homoserine
glycine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-homoserine
glycine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-methionine
glycine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-methionine
glycine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-methionine
glycine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
?
glyoxylate + L-methionine (SR)-sulfoxide
glycine + ?
show the reaction diagram
-
-
-
?
glyoxylate + L-methionine (SR)-sulfoxide
glycine + ?
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
?
glyoxylate + L-methionine sulfone
glycine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-methionine sulfone
glycine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
glyoxylate + L-phenylalanine
glycine + phenylpyruvate
show the reaction diagram
-
-
-
?
glyoxylate + L-phenylalanine
glycine + phenylpyruvate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-serine
glycine + 3-hydroxy-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + S-methyl-L-cysteine
glycine + 3-methylsulfanyl-2-oxopropanoate
show the reaction diagram
-
-
-
?
glyoxylate + S-methyl-L-cysteine
glycine + 3-methylsulfanyl-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
L-albizziin + glyoxylate
glycine + 2-imidazolidone-5-hydroxy-5-carboxylic acid
show the reaction diagram
-
-
undergoes non-enzymatic dehydration to 2-imidazolinone-5-carboxylate
?
L-albizziin + glyoxylate
glycine + 2-imidazolidone-5-hydroxy-5-carboxylic acid
show the reaction diagram
-
S-carbamoyl-L-cysteine or O-carbamoyl-L-serine can replace albizziin
-
?
L-glutamine + 2-oxoglutaramate
2-oxoglutaramate + L-glutamine
show the reaction diagram
-
-
-
?
L-glutamine + 2-oxoglutaramate
2-oxoglutaramate + L-glutamine
show the reaction diagram
-
-
-
?
L-glutamine + 3-hydroxypyruvate
2-oxoglutaramate + L-serine
show the reaction diagram
-
-
-
?
L-glutamine + 3-mercaptopyruvate
2-oxoglutaramate + L-cysteine
show the reaction diagram
-
-
-
?
L-glutamine + 3-mercaptopyruvate
2-oxoglutaramate + L-cysteine
show the reaction diagram
-
-
-
?
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
2-oxoglutaramate + L-methionine
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
Rattus norvegicus Sprague-Dawley
-
-
-
?
L-glutamine + pyruvate
2-oxoglutaramate + L-alanine
show the reaction diagram
-
-
-
?
L-phenylalanine + 4-methylthio-2-oxobutanoate
phenylpyruvate + 2-amino-4-methylthiobutanoate
show the reaction diagram
-
-
-
-
-
pyruvate + L-4-glutamylmethylamide
L-alanine + ?
show the reaction diagram
-
-
-
?
pyruvate + L-glutamine
L-alanine + 2-oxoglutaramate
show the reaction diagram
-
-
-
?
pyruvate + L-glutamine
L-alanine + 2-oxoglutaramate
show the reaction diagram
-
pyruvate cannot be replaced by its hydrazone, oxime or semicarbazone derivatives
-
?
pyruvate + L-glutamine
L-alanine + 2-oxoglutaramate
show the reaction diagram
-
L-glutamine cannot be replaced by glycine
-
?
pyruvate + L-methionine
L-alanine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
pyruvate + L-methionine (SR)-sulfoxide
L-alanine + 4-methylsulfinyl-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
pyruvate + L-methionine sulfone
L-alanine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
pyruvate + L-phenylalanine
L-alanine + phenylpyruvate
show the reaction diagram
-
-
-
?
pyruvate + L-phenylalanine
L-alanine + phenylpyruvate
show the reaction diagram
-
-
-
?
pyruvate + L-phenylalanine
L-alanine + phenylpyruvate
show the reaction diagram
-
-
-
?
S-(3-aminopropyl)cysteine + pyruvate
S-(3-aminopropyl)thiopyruvic acid + amino acid
show the reaction diagram
-
-
-
?
selenium-(3-aminopropyl)selenocysteine + pyruvate
selenium-(3-aminopropyl)selenopyruvic acid + amino acid
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
-
-
-
?
L-glutamine + pyruvate
2-oxoglutaramate + L-alanine
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-oxoisopentanoate
-
transamination between glutamine and glyoxylate
3-Mercaptopyruvate
-
-
4-methylsulfanyl-2-oxobutanoate
-
-
Aminoxyacetate
-
soluble fraction
Carbonyl reagents
-
-
-
Cycloserine
-
soluble fraction
glyoxylate
-
at high concentration, transamination between albizziin and glyoxylate
hydroxylamine
-
soluble fraction
hydroxylamine
-
-
Isonicotinic acid hydrazide
-
-
L-gamma-glutamylhydrazide
-
with gamma-glutamylhydrazone of glyoxylate as substrate, weak
L-glutamine
-
with gamma-glutamylhydrazone of glyoxylate as substrate
Sodium borohydride
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
50
-
2-Oxoglutaramate
-
pH 8.4, 37C
8
-
3-hydroxypyruvate
-
pH 8.4, 37C
4.8
-
3-Mercaptopyruvate
-
pH 8.4, 37C
3.2
-
4-methylsulfanyl-2-oxobutanoate
-
pH 8.4, 37C
23
-
gamma-glutamylhydrazone of glyoxylate
-
pH 8.4, 37C
-
8
-
glyoxylate
-
pH 8.4, 37C
4.4
-
L-glutamic acid gamma-methylester
-
pH 8.4, 37C
2
-
L-glutamine
-
pH 8.4, 37C
6
-
L-glutamine
-
pH 8.4, 37C
1.9
-
L-methionine
-
pH 8.4, 37C
2
-
L-methionine (SR)-sulfoxide
-
pH 8.4, 37C
10.5
-
pyruvate
-
pH 8.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.013
-
-
-
0.113
-
-
mitochondrion
5
-
-
soluble fraction
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.6
8.8
-
about half-maximum activity at pH 7.6 and about 90% of maximum activity at pH 8.8
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
isoform +14KATI
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
additional information
-
most isoforms are ubiquitously expressed
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
110000
-
-
-
110000
-
-
PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 44000, SDS-PAGE
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
in crude extract inactivation within 10 min, in the presence of pyruvate, glyoxylate or phenylpyruvate 1 h stability, 25% loss of activity within 3 h
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2-mercaptoethanol and alpha-keto acids stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, at least 7 weeks
-
0-5C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, little or no loss of activity for 1-2 weeks, in the absence of mercaptoethanol inactivation within 1-2 days
-
24-26C, 20% loss of activity of purified enzyme in 3 days
-
4C, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ammonium sulfate fractionation , DE-52 chromatography
-
DE-52 chromatography, hydroxyapatite chromatography
-
DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4 cDNAs with different 5-untranslated regions
-