Information on EC 2.6.1.15 - glutamine-pyruvate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.15
-
RECOMMENDED NAME
GeneOntology No.
glutamine-pyruvate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-glutamine + pyruvate = 2-oxoglutaramate + L-alanine
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
-
-
glutamate and glutamine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
L-glutamine:pyruvate aminotransferase
A pyridoxal-phosphate protein. L-Methionine can act as donor; glyoxylate can act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-44-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxoglutaramate + glycine
L-glutamine + glyoxylate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-alanine
L-glutamine + pyruvate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-asparagine
L-glutamine + 2-oxosuccinamate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-cysteine
L-glutamine + 3-mercapto-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-ethionine
L-glutamine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-glutamic acid 4-benzylester
L-glutamine + 2-oxopentane-1,5-dioic acid benzyl ester
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-homoserine
L-glutamine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-methionine
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
2-oxoglutaramate + L-methionine (SR)-sulfoxide
L-glutamine + 4-methylsulfinyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-methionine sulfone
L-glutamine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
-
-
-
?
2-oxoglutaramate + L-phenylalanine
L-glutamine + phenylpyruvate
show the reaction diagram
-
poor substrate
-
?
2-oxoglutaramate + L-serine
L-glutamine + 3-hydroxy-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
gamma-glutamylhydrazones of 2-oxo acid
L-amino acid + 2-hydroxy-tetrahydro-6-pyridazinone-3-carboxylic acid
show the reaction diagram
glyoxylate + 4-glutamylmethylamide
glycine + ?
show the reaction diagram
glyoxylate + DL-homocysteine
glycine + 2-oxo-4-thiobutanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + glutamic acid 4-methyl ester
glycine + 2-oxoglutarate 4-methyl ester
show the reaction diagram
glyoxylate + L-2-amino-4-oxo-5-chloropentanoic acid
glycine + 5-chloro-2,4-dioxopentanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-alanine
glycine + pyruvate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-asparagine
glycine + 2-oxosuccinamate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + L-cysteine
glycine + 3-mercapto-2-oxopropanoate
show the reaction diagram
glyoxylate + L-ethionine
glycine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
glyoxylate + L-glutamine
glycine + 2-oxoglutaramate
show the reaction diagram
glyoxylate + L-homoserine
glycine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
glyoxylate + L-methionine
glycine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
glyoxylate + L-methionine (SR)-sulfoxide
glycine + ?
show the reaction diagram
glyoxylate + L-methionine sulfone
glycine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
glyoxylate + L-phenylalanine
glycine + phenylpyruvate
show the reaction diagram
glyoxylate + L-serine
glycine + 3-hydroxy-2-oxopropanoate
show the reaction diagram
-
poor substrate
-
?
glyoxylate + S-methyl-L-cysteine
glycine + 3-methylsulfanyl-2-oxopropanoate
show the reaction diagram
L-albizziin + glyoxylate
glycine + 2-imidazolidone-5-hydroxy-5-carboxylic acid
show the reaction diagram
L-glutamine + 2-oxoglutaramate
2-oxoglutaramate + L-glutamine
show the reaction diagram
L-glutamine + 3-hydroxypyruvate
2-oxoglutaramate + L-serine
show the reaction diagram
-
-
-
?
L-glutamine + 3-mercaptopyruvate
2-oxoglutaramate + L-cysteine
show the reaction diagram
L-glutamine + 4-methylsulfanyl-2-oxobutanoate
2-oxoglutaramate + L-methionine
show the reaction diagram
-
-
-
?
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
L-glutamine + pyruvate
2-oxoglutaramate + L-alanine
show the reaction diagram
-
-
-
?
L-phenylalanine + 4-methylthio-2-oxobutanoate
phenylpyruvate + 2-amino-4-methylthiobutanoate
show the reaction diagram
-
-
-
-
-
pyruvate + L-4-glutamylmethylamide
L-alanine + ?
show the reaction diagram
-
-
-
?
pyruvate + L-glutamine
L-alanine + 2-oxoglutaramate
show the reaction diagram
pyruvate + L-methionine
L-alanine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
pyruvate + L-methionine (SR)-sulfoxide
L-alanine + 4-methylsulfinyl-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
pyruvate + L-methionine sulfone
L-alanine + 4-methylsulfonyl-2-oxobutanoate
show the reaction diagram
-
poor substrate
-
?
pyruvate + L-phenylalanine
L-alanine + phenylpyruvate
show the reaction diagram
S-(3-aminopropyl)cysteine + pyruvate
S-(3-aminopropyl)thiopyruvic acid + amino acid
show the reaction diagram
-
-
-
?
selenium-(3-aminopropyl)selenocysteine + pyruvate
selenium-(3-aminopropyl)selenopyruvic acid + amino acid
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-glutamine + alpha-keto acid
alpha-ketoglutarate + amino acid
show the reaction diagram
L-glutamine + pyruvate
2-oxoglutaramate + L-alanine
show the reaction diagram
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoisopentanoate
-
transamination between glutamine and glyoxylate
3-Mercaptopyruvate
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-
4-methylsulfanyl-2-oxobutanoate
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-
Aminoxyacetate
-
soluble fraction
Carbonyl reagents
-
-
-
cycloserine
-
soluble fraction
glyoxylate
-
at high concentration, transamination between albizziin and glyoxylate
hydroxylamine
Isonicotinic acid hydrazide
-
-
L-gamma-glutamylhydrazide
-
with gamma-glutamylhydrazone of glyoxylate as substrate, weak
L-glutamine
-
with gamma-glutamylhydrazone of glyoxylate as substrate
Sodium borohydride
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50
2-Oxoglutaramate
-
pH 8.4, 37C
8
3-hydroxypyruvate
-
pH 8.4, 37C
4.8
3-Mercaptopyruvate
-
pH 8.4, 37C
3.2
4-methylsulfanyl-2-oxobutanoate
-
pH 8.4, 37C
23
gamma-glutamylhydrazone of glyoxylate
-
pH 8.4, 37C
-
8
glyoxylate
-
pH 8.4, 37C
4.4
L-glutamic acid gamma-methylester
-
pH 8.4, 37C
2 - 6
L-glutamine
1.9
L-methionine
-
pH 8.4, 37C
2
L-methionine (SR)-sulfoxide
-
pH 8.4, 37C
10.5
pyruvate
-
pH 8.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.113
-
mitochondrion
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2 - 8.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6 - 8.8
-
about half-maximum activity at pH 7.6 and about 90% of maximum activity at pH 8.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
isoform +14KATI
Manually annotated by BRENDA team
additional information
-
most isoforms are ubiquitously expressed
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 44000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
in crude extract inactivation within 10 min, in the presence of pyruvate, glyoxylate or phenylpyruvate 1 h stability, 25% loss of activity within 3 h
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol and alpha-keto acids stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, at least 7 weeks
-
0-5C, in 30-50 mM potassium phosphate buffer, pH 7.2, 1 mM 2-mercaptoethanol, little or no loss of activity for 1-2 weeks, in the absence of mercaptoethanol inactivation within 1-2 days
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24-26C, 20% loss of activity of purified enzyme in 3 days
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4C, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation , DE-52 chromatography
-
DE-52 chromatography, hydroxyapatite chromatography
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DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4 cDNAs with different 5-untranslated regions
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