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Literature summary for 2.6.1.15 extracted from
- Glutamine transaminase L from rat liver (1985), Methods Enzymol., 113, 338-343.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminoxyacetate | soluble fraction | Rattus norvegicus |  |
| cycloserine | soluble fraction | Rattus norvegicus | |
| hydroxylamine | soluble fraction | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DE-52 chromatography, hydroxyapatite chromatography for soluble fraction, DE-52 chromatography for mitochondrial fraction | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.113 | - |
mitochondrion | Rattus norvegicus |
| 5 | - |
soluble fraction | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4ºC, 10 mM potassium phosphate, pH 7.2, 10% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM pyridoxal 5'-phosphate, 3 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + L-glutamine | best substrate | Rattus norvegicus | glycine + 2-oxoglutaramate | - |
? | |
| glyoxylate + L-methionine | - |
Rattus norvegicus | glycine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
| L-glutamine + 2-oxoglutaramate | - |
Rattus norvegicus | 2-oxoglutaramate + L-glutamine | - |
? | |
| L-glutamine + 3-mercaptopyruvate | - |
Rattus norvegicus | 2-oxoglutaramate + L-cysteine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
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Release 2023.1 (January 2023)
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