Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.4.2.44 - S-methyl-5'-thioinosine phosphorylase and Organism(s) Pseudomonas aeruginosa and UniProt Accession Q9HZK1

for references in articles please use BRENDA:EC2.4.2.44
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.44 S-methyl-5'-thioinosine phosphorylase
IUBMB Comments
No activity with S-methyl-5'-thioadenosine. The catabolism of of 5'-methylthioadenosine in Pseudomonas aeruginosa involves deamination to S-methyl-5'-thioinosine (EC 3.5.4.31, S-methyl-5'-thioadenosine deaminase) and phosphorolysis to hypoxanthine .
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: Q9HZK1
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
pa3004, mti phosphorylase, methylthioinosine phosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methylthioinosine phosphorylase
-
MTI phosphorylase
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5'-thioinosine:phosphate S-methyl-5-thio-alpha-D-ribosyl-transferase
No activity with S-methyl-5'-thioadenosine. The catabolism of of 5'-methylthioadenosine in Pseudomonas aeruginosa involves deamination to S-methyl-5'-thioinosine (EC 3.5.4.31, S-methyl-5'-thioadenosine deaminase) and phosphorolysis to hypoxanthine [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
adenosine + phosphate
adenine + alpha-D-ribose 1-phosphate
show the reaction diagram
weak substrate
-
-
?
adenosine + phosphate
adenine + D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
show the reaction diagram
the catalytic efficiency (kcat/Km) for S-methyl-5'-thioinosine is 290 times larger than for inosine
-
-
?
inosine + phosphate
hypoxanthine + D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
S-methyl-5'-thioinosine + phosphate
hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-methyl-5'-thioinosine + phosphate
hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
the enzyme is involved in catabolism of 5'-methylthioadenosine in Pseudomonas aeruginosa. S-Methyl-5'-thioadenosine is converted to S-methyl-5'-thioinosine and hypoxanthine but not to adenine
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
5'-methylthio-immucillin-H
-
5'-phenylthio-immucillin-H
-
S-methyl-5'-thioadenosine
competitive inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
5'-methylthioinosine
pH 7.4, 25°C
0.023
adenine
pH 7.4, 25°C
0.023
adenosine
pH 7.4, 25°C
0.09
Inosine
pH 7.4, 25°C
0.0026
S-methyl-5'-thioinosine
pH 7.4, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.8
5'-methylthioinosine
pH 7.4, 25°C
0.0549
adenine
pH 7.4, 25°C
0.0549
adenosine
pH 7.4, 25°C
0.57
Inosine
pH 7.4, 25°C
4.8
S-methyl-5'-thioinosine
pH 7.4, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24
adenine
pH 7.4, 25°C
6.3
Inosine
pH 7.4, 25°C
1846
S-methyl-5'-thioinosine
pH 7.4, 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00000034 - 0.0000008
5'-methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
0.000000076 - 0.00000084
5'-methylthio-immucillin-H
0.000000035 - 0.00000066
5'-phenylthio-immucillin-H
0.07
S-methyl-5'-thioadenosine
pH 7.4, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotrimer
crystal structure
trimer
the crystal structure in complex with hypoxanthine, determined to 2.8 A resolution reveals a 3-fold symmetric homotrimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallization of recombinant enzyme (9 mg/ml) in 50 mM HEPES, pH 7.4, 30% polyethylene glycol monomethyl ether 2000, 0.1 M potassium thiocyanate in the presence of 5 mM S-methyl-5'-thioinosine and 5 mM sulfate by sitting-drop vapor diffusion. Crystal structure of the enzyme in complex with hypoxanthine is determined to 2.8 A resolution
the crystal structure of P. aeruginosa MTIP (PaMTIP) in complex with hypoxanthine is determined to 2.8 A resolution and reveals a three-fold symmetric homotrimer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Guan, R.; Ho, M.C., Almo, S.C.; Schramm, V.L.
Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing
Biochemistry
50
1247-1254
2011
Pseudomonas aeruginosa (Q9HZK1), Pseudomonas aeruginosa
Manually annotated by BRENDA team