Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
expressed in Escherichia coli as a His-tagged fusion protein | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
crystallization of recombinant enzyme (9 mg/ml) in 50 mM HEPES, pH 7.4, 30% polyethylene glycol monomethyl ether 2000, 0.1 M potassium thiocyanate in the presence of 5 mM S-methyl-5'-thioinosine and 5 mM sulfate by sitting-drop vapor diffusion. Crystal structure of the enzyme in complex with hypoxanthine is determined to 2.8 A resolution | Pseudomonas aeruginosa |
the crystal structure of P. aeruginosa MTIP (PaMTIP) in complex with hypoxanthine is determined to 2.8 A resolution and reveals a three-fold symmetric homotrimer | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | - |
Pseudomonas aeruginosa | |
5'-methylthio-immucillin-H | - |
Pseudomonas aeruginosa | |
5'-phenylthio-immucillin-H | - |
Pseudomonas aeruginosa | |
S-methyl-5'-thioadenosine | competitive inhibitor | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
5'-methylthioinosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.0026 | - |
S-methyl-5'-thioinosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.023 | - |
adenosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.023 | - |
adenine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.09 | - |
Inosine | pH 7.4, 25°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-methyl-5'-thioinosine + phosphate | Pseudomonas aeruginosa | the enzyme is involved in catabolism of 5'-methylthioadenosine in Pseudomonas aeruginosa. S-Methyl-5'-thioadenosine is converted to S-methyl-5'-thioinosine and hypoxanthine but not to adenine | hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HZK1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioinosine + phosphate | - |
Pseudomonas aeruginosa | hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | weak substrate | Pseudomonas aeruginosa | adenine + alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Pseudomonas aeruginosa | adenine + D-ribose 1-phosphate | - |
? | |
inosine + phosphate | the catalytic efficiency (kcat/Km) for S-methyl-5'-thioinosine is 290 times larger than for inosine | Pseudomonas aeruginosa | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Pseudomonas aeruginosa | hypoxanthine + D-ribose 1-phosphate | - |
? | |
additional information | S-methyl-5'-thioadenosine is not a substrate | Pseudomonas aeruginosa | ? | - |
? | |
S-methyl-5'-thioinosine + phosphate | the enzyme is involved in catabolism of 5'-methylthioadenosine in Pseudomonas aeruginosa. S-Methyl-5'-thioadenosine is converted to S-methyl-5'-thioinosine and hypoxanthine but not to adenine | Pseudomonas aeruginosa | hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? | |
S-methyl-5'-thioinosine + phosphate | the methylthio group of S-methyl-5'-thioinosine is important for both substrate binding and catalysis | Pseudomonas aeruginosa | hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | crystal structure | Pseudomonas aeruginosa |
trimer | the crystal structure in complex with hypoxanthine, determined to 2.8 A resolution reveals a 3-fold symmetric homotrimer | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
methylthioinosine phosphorylase | - |
Pseudomonas aeruginosa |
MTI phosphorylase | - |
Pseudomonas aeruginosa |
MTIP | - |
Pseudomonas aeruginosa |
PA3004 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0549 | - |
adenosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.0549 | - |
adenine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.57 | - |
Inosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
4.8 | - |
5'-methylthioinosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
4.8 | - |
S-methyl-5'-thioinosine | pH 7.4, 25°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000035 | - |
5'-phenylthio-immucillin-H | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.000000076 | - |
5'-methylthio-immucillin-H | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.00000034 | - |
5'-methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.00000066 | - |
5'-phenylthio-immucillin-H | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.0000008 | - |
5'-methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.00000084 | - |
5'-methylthio-immucillin-H | pH 7.4, 25°C | Pseudomonas aeruginosa | |
0.07 | - |
S-methyl-5'-thioadenosine | pH 7.4, 25°C | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
adenine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
6.3 | - |
Inosine | pH 7.4, 25°C | Pseudomonas aeruginosa | |
1846 | - |
S-methyl-5'-thioinosine | pH 7.4, 25°C | Pseudomonas aeruginosa |