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Information on EC 2.4.1.207 - xyloglucan:xyloglucosyl transferase and Organism(s) Brassica oleracea var. botrytis and UniProt Accession Q6YDN9
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2.4.1.207 xyloglucan:xyloglucosyl transferaseIUBMB Comments
Does not use cello-oligosaccharides as either donor or acceptor.
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The taxonomic range for the selected organisms is: Brassica oleracea var. botrytis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
2
=
+
2
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+
Synonyms
sixth, xyloglucan endotransglycosylase, xyloglucan endotransglucosylase/hydrolase, xyloglucan endotransglucosylase, xyloglucan endotransglycosylase/hydrolase, xth31, xyloglucan endo-transglycosylase, atxth21, xth33, xth19, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
endo-xyloglucan transferase
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endoxyloglucan transferase
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EXGT
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EXT
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GenBank L46792-derived protein GI 950299
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GenBank X93173-derived protein GI 1890573
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GenBank X93174-derived protein GI 1890575
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Genbank X93175-derived protein GI 1890577
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GenBank Z97335-derived protein GI
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NXET
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XET
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xyloglucan endotransglycosylase
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xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
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xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
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xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
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xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
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xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
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xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana)
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xyloglucan recombinase
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xyloglucan X93174-derived protein GI 1890575
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xyloglucan-specific endo-(1->4)-beta-D-glucanase
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xyloglucan:xyloglucanotransferase
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xyloglucanotransferase, xyloglucan (xyloglucan donor)
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor)
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced)
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5)
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA)
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xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB)
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additional information
the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan
enzyme possesss the catalytic sequence motif EIDFE conserved in the glycosyl hydrolase family 16
SYSTEMATIC NAME
IUBMB Comments
xyloglucan:xyloglucan xyloglucanotransferase
Does not use cello-oligosaccharides as either donor or acceptor.
CAS REGISTRY NUMBER
COMMENTARY
141588-40-1
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172279-31-1
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Arabidopsis thaliana gene TCH4 precursor reduced) /xyloglucan endotransglycosylase (Arabidopsis thaliana gene TCH4 precursor reduced)
202012-34-8
xyloglucan endotransglycosylase-related protein XTR-7 (Arabidopsis thaliana) /genBank Z97335-derived protein GI 2244769
203402-40-8
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone PM5 gene HVPM5) /genBank X93173-derived protein GI 1890573 /xyloglucan endotransglycosylase (barley clone PM5 gene HVPM5)
203402-41-9
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEA gene HVXEA) /genBank X93174-derived protein GI 1890575 /xyloglucan X93174-derived protein GI 1890575 /xyloglucan endotransglycosylase (barley clone XEA gene HVXEA)
203402-42-0
xyloglucanotransferase, xyloglucan (xyloglucan donor) (barley clone XEB gene HVXEB) /genbank X93175-derived protein GI 1890577 /xyloglucan endotransglycosylase (barley clone XEB gene HVXEB)
205513-08-2
xyloglucanotransferase, xyloglucan (xyloglucan donor) (Actinia chinensis clone AdXET-5 gene XET precursor) /genBank L46792-derived protein GI 950299 /Xyloglucan endotransglycosylase (Actinia deliciosa strain Hayward pericarp clone AdXET-5 gene XET precursor)
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
different assay methods, overview
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-
?
additional information
?
-
-
different assay methods, overview
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
glycosylation is required for activity, deglycosylation results in 40% loss of activity
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additional information
-
glycosylation is required for activity, deglycosylation results in 40% loss of activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
XTH-activating factor
XAF, present in CHPs works by promoting (e.g. allosterically) XET activity or by re-solubilising sequestered XTH proteins
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additional information
glycosylation is required for activity, deglycosylation results in 40% loss of activity
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additional information
-
glycosylation is required for activity, deglycosylation results in 40% loss of activity
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additional information
certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures, can re-solubilise the bound enzyme, re-activating it
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additional information
-
certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures, can re-solubilise the bound enzyme, re-activating it
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
different assay methods, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity
additional information
-
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10 - 50
about 20% of maximal activity at 10°C and 50°C, about 50% of maximal activity at 20°C and 48°C, temperature-profile
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
xyloglucan endotransglucosylase (XET) activity, which cuts and re-joins hemicellulose chains in the plant cell wall, contributing to wall assembly and growth regulation, is the major activity of XTH proteins. During purification, XTHs often lose XET activity which, however, is restored by treatment with certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, mung bean shoots, and Arabidopsis cell-suspension cultures
additional information
additional information
XTHs in dilute solution bind to diverse surfaces (e.g. glass and cellulose), and certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, can re-solubilise the bound enzyme, re-activating it. Cell walls prepared from cauliflower florets, mung bean shoots and Arabidopsis cell-suspension cultures each contain endogenous, tightly bound, inactive XTHs, which are likewise rapidly solubilised and thus activated by cauliflower XTH-activating factor (XAF). Analysis of XTH-activating factor (XAF) activity of CHP preparations from diverse plant sources, CHPs from all plants tested possess XAF activity, overview
additional information
-
XTHs in dilute solution bind to diverse surfaces (e.g. glass and cellulose), and certain cold-water-extractable, heat-stable polymers (CHPs), e.g. from cauliflower florets, can re-solubilise the bound enzyme, re-activating it. Cell walls prepared from cauliflower florets, mung bean shoots and Arabidopsis cell-suspension cultures each contain endogenous, tightly bound, inactive XTHs, which are likewise rapidly solubilised and thus activated by cauliflower XTH-activating factor (XAF). Analysis of XTH-activating factor (XAF) activity of CHP preparations from diverse plant sources, CHPs from all plants tested possess XAF activity, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). XTH_BRAOB
295
1
34105
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
31737
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
33115
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
one N-glycosylation site situated close to the predicted catalytic residues, enzyme is a high-mannose-type glycan, glycosylation is required for activity, deglycosylation results in 40% loss of activity, overview
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
about 30% activity is lost during one freeze-thaw cycle
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
14°C, 0.1 mg/ml enzyme in 50 mM ammonium acetate, pH 5.5, with 0.5 M ammonium sulfate, complete loss of activity within 3 months
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from florets to homogeneity by adsorption chromatography, 55-140fold purification dependent on different assay methods for quantification
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Pichia pastoris resulting in a recombinant enzyme which is more highly mannosylated than the native enzyme without having influence on activity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Henriksson, H.; Denman, S.E.; Campuzano, I.D.; Ademark, P.; Master, E.R.; Teeri, T.T.; Brumer, H.
N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A
Biochem. J.
375
61-73
2003
Brassica oleracea var. botrytis (Q6YDN9), Brassica oleracea var. botrytis
Sharples, S.C.; Nguyen-Phan, T.C.; Fry, S.C.
Xyloglucan endotransglucosylase/hydrolases (XTHs) are inactivated by binding to glass and cellulosic surfaces, and released in active form by a heat-stable polymer from cauliflower florets
J. Plant Physiol.
218
135-143
2017
Arabidopsis thaliana (P24806), Vigna radiata (Q5MB21), Brassica oleracea var. botrytis (Q6YDN9), Brassica oleracea var. botrytis
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