Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | glycosylation is required for activity, deglycosylation results in 40% loss of activity | Brassica oleracea var. botrytis |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Pichia pastoris resulting in a recombinant enzyme which is more highly mannosylated than the native enzyme without having influence on activity | Brassica oleracea var. botrytis |
General Stability | Organism |
---|---|
about 30% activity is lost during one freeze-thaw cycle | Brassica oleracea var. botrytis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | glycosylation is required for activity, deglycosylation results in 40% loss of activity | Brassica oleracea var. botrytis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31737 | - |
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry | Brassica oleracea var. botrytis |
33115 | - |
x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry | Brassica oleracea var. botrytis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea var. botrytis | Q6YDN9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | one N-glycosylation site situated close to the predicted catalytic residues, enzyme is a high-mannose-type glycan, glycosylation is required for activity, deglycosylation results in 40% loss of activity, overview | Brassica oleracea var. botrytis |
Purification (Comment) | Organism |
---|---|
native enzyme from florets to homogeneity by adsorption chromatography, 55-140fold purification dependent on different assay methods for quantification | Brassica oleracea var. botrytis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc | enzyme possesss the catalytic sequence motif EIDFE conserved in the glycosyl hydrolase family 16 | Brassica oleracea var. botrytis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
floret | - |
Brassica oleracea var. botrytis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
different assay methods, overview | Brassica oleracea var. botrytis |
0.812 | - |
purified enzyme, radioactive assay method | Brassica oleracea var. botrytis |
Storage Stability | Organism |
---|---|
14°C, 0.1 mg/ml enzyme in 50 mM ammonium acetate, pH 5.5, with 0.5 M ammonium sulfate, complete loss of activity within 3 months | Brassica oleracea var. botrytis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | different assay methods, overview | Brassica oleracea var. botrytis | ? | - |
? | |
xyloglucan oligosaccharides + xyloglucan | - |
Brassica oleracea var. botrytis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33115, native enzyme, mass spectrometry, x * 31737, deglycosyated enzyme, mass spectrometry | Brassica oleracea var. botrytis |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the glycosyl hydrolase family 16, i.e. GH16 | Brassica oleracea var. botrytis |
XET | - |
Brassica oleracea var. botrytis |
xyloglucan endotransglycosylase 16A | - |
Brassica oleracea var. botrytis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Brassica oleracea var. botrytis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | about 20% of maximal activity at 10°C and 50°C, about 50% of maximal activity at 20°C and 48°C, temperature-profile | Brassica oleracea var. botrytis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Brassica oleracea var. botrytis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-profile, sigmoidal decline in activity at high pH, at low pH occurs an abrupt drop in activity | Brassica oleracea var. botrytis |