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Information on EC 2.4.1.10 - levansucrase and Organism(s) Gluconacetobacter diazotrophicus and UniProt Accession Q43998
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2.4.1.10 levansucraseIUBMB Comments
Some other sugars can act as D-fructosyl acceptors.
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Word Map
- 2.4.1.10
-
fructosylation
- fructooligosaccharides
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transfructosylation
- mobilis
-
zymomonas
- exopolysaccharide
- erwinia
- raffinose
-
prebiotics
- inulin
-
levan-type
- diazotrophicus
- fructosyltransferases
- leuconostoc
- reuteri
- inulosucrase
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gluconacetobacter
- mesenteroides
- dextransucrase
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sucrose:sucrose
- synthesis
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gluconobacter
- 6-kestose
- aquatilis
-
rahnella
-
exocellular
- invertases
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hpaec-pad
-
sourdough
-
amylovoran
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antitermination
- oxydans
- food industry
- agriculture
The taxonomic range for the selected organisms is: Gluconacetobacter diazotrophicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
levansucrase, 6-sft, sucrose:fructan 6-fructosyltransferase, fructansucrase, lsc-3, t2-ls, t1-ls, sucrose 6-fructosyltransferase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-2,6-fructan:D-glucose 1-fructosyltransferase
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-
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beta-2,6-fructosyltransferase
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-
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fructosyltransferase, sucrose 6-
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sucrose 6-fructosyltransferase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
Asp309 is involved in catalytic ping-pong mechanism, localization in conserved RDP-motif
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
ping-pong mechanism
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sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside
formation of a transient fructosyl-enzyme intermediate, kinetics of the hydrolase, exchange and transfructosylation reaction
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
sucrose:[6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside 6-beta-D-fructosyltransferase
Some other sugars can act as D-fructosyl acceptors.
CAS REGISTRY NUMBER
COMMENTARY
9030-17-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
beta-D-fructofuranosidase activity
-
?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
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-
-
?
sucrose + D-glucose
glucose + sucrose
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exchange reaction
-
?
additional information
?
-
levan synthesis, the first reaction of levan synthesis is formation of 6-kestose from two molecules of sucrose, one acting as a fructosyl donor and the other as an acceptor. 6-Kestose is further extended through numerous transfructosylation reactions
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-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
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-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
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-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
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various acceptors
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-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
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synthesis of a high-molecular-mass fructan of the levan type
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
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translocation of the enzyme is a rate-limiting step
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
-
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
-
synthesis of a high-molecular-mass fructan of the levan type
-
?
sucrose + (2,6-beta-D-fructosyl)n
glucose + (2,6-beta-D-fructosyl)n+1
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translocation of the enzyme is a rate-limiting step
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
proteinase K
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90% remaining activity after incubation at 37°C for 1 h
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
recombinant enzyme expressed in Escherichia coli
UniProt
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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signal peptide dependent secretion mechanism
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recombinant enzyme expressed in Pichia pastoris, 18%
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precursor form, before secretion into the medium, maturation includes cleavage of 30 amino acid residue signal peptide and formation of a pyroglutamic acid residue, not attached to membranes
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recombinant enzyme expressed in Pichia pastoris, 81%
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
in addition to fructooligosaccharides, levansucrases produce polymeric levan, degree of polymerization of which can be very high. Enzyme LsdA is specifically prone for fructooligosaccharide synthesis, producing inulin-type fructooligosaccharides and only a small amount of levan
additional information
the catalytic triad is formed by residues Asp135, Asp309 and Glu401
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
alignment of RDP-motif sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic center of levansucrase LsdA, PDB ID 1W18, with raffinose molecule, from PDB ID 3BYN, in the substrate-binding pocket
the three-dimensional structure of LsdA determined by X-ray crystallography at a resolution of 2.5 A
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C339S
kcat/Km for hydrolysis of sucrose is 68.75fold lower than wild-type value
C395S
kcat/Km for hydrolysis of sucrose is 60fold lower than wild-type value
D135N
kcat/Km for hydrolysis of sucrose is 1257fold lower than wild-type value
D309N
site-directed mutagenesis of RDP-motif, no affection of enzyme secretion but hydrolysis activity, 13fold increase of kcat, 71fold decrease of kcat/Km, unaltered Km for sucrose
additional information
additional information
construction of mutants AD6, AD8, and AD9, that are unable to secrete LsdA due to nptII insertions in the type II secretion, gsp, operon. None of the mutants released extracellular LsdB, the exolavanase organized in one operon with the levansucrase LsdA
additional information
-
construction of mutants AD6, AD8, and AD9, that are unable to secrete LsdA due to nptII insertions in the type II secretion, gsp, operon. None of the mutants released extracellular LsdB, the exolavanase organized in one operon with the levansucrase LsdA
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant and native wild-type and recombinant mutant from supernatant
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression of LsdA in a levansucrase deficient Acetobacter diazotrophicus mutant strain
gene lsdA, organized in an operon with exolevanase LsdB, transcriptional linkage of the chromosomal lsdAB gene cluster
expression in Acetobacter azotrophicus wild-type strain SRT4, 6fold increase in periplasm, 1.7fold increase of extracellular enzyme
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expression of truncated precursor protein in Pichia pastoris under control of Pichia pastoris-AOX1 promotor, methanol induction, increased thermal stability
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Trujillo, L.E.; Arrieta, J.G.; Dafhnis, F.; Garcia, J.; Valdes, J.; Tambara, Y.; Perez, M.; Hernandez, L.
Fructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris
Enzyme Microb. Technol.
28
139-144
2001
Gluconacetobacter diazotrophicus
Hernandez, L.; Arrieta, J.; Betancourt, L.; Falcon, V.; Madrazo, J.; Coego, A.; Menendez, C.
Levansucrase from Acetobacter diazotrophicus SRT4 is secreted via periplasm by a signal-peptide-dependent pathway
Curr. Microbiol.
39
146-152
1999
Gluconacetobacter diazotrophicus
Batista, F.R.; Hernandez, L.; Fernandez, J.R.; Arrieta, J.; Menendez, C.; Gomez, R.; Tambara, Y.; Pons, T.
Substitution of Asp-309 by Asn in the Arg-Asp-Pro (RDP) motif of Acetobacter diazotrophicus levansucrase affects sucrose hydrolysis, but not enzyme specificity
Biochem. J.
337
503-506
1999
Gluconacetobacter diazotrophicus (Q43998)
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Hernandez, L.; Arrieta, J.; Menendez, C.; Vazquez, R.; Coego, A.; Suarez, V.; Selman, G.; Petit-Glatron, M.F.; Chambert, R.
Isolation and enzymic properties of levansucrase secreted by Acetobacter diazotrophicus SRT4, a bacterium associated with sugar cane
Biochem. J.
309
113-118
1995
Gluconacetobacter diazotrophicus
-
Martinez-Fleites, C.; Ortiz-Lombardia, M.; Pons, T.; Tarbouriech, N.; Taylor, E.J.; Arrieta, J.G.; Hernandez, L.; Davies, G.J.
Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus
Biochem. J.
390
19-27
2005
Gluconacetobacter diazotrophicus (Q43998), Gluconacetobacter diazotrophicus, Gluconacetobacter diazotrophicus SRT4 (Q43998), Gluconacetobacter diazotrophicus SRT4
van Hijum, S.A.; Kralj, S.; Ozimek, L.K.; Dijkhuizen, L.; van Geel-Schutten, I.G.
Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria
Microbiol. Mol. Biol. Rev.
70
157-176
2006
Gluconacetobacter diazotrophicus, Lactobacillus sp., Streptococcus salivarius, Streptococcus sp., Zymomonas mobilis, Bacillus subtilis (P05655)
Menendez, C.; Banguela, A.; Caballero-Mellado, J.; Hernandez, L.
Transcriptional regulation and signal-peptide-dependent secretion of exolevanase (LsdB) in the endophyte Gluconacetobacter diazotrophicus
Appl. Environ. Microbiol.
75
1782-1785
2009
Gluconacetobacter diazotrophicus (Q43998), Gluconacetobacter diazotrophicus
Visnapuu, T.; Mardo, K.; Alamaee, T.
Levansucrases of a Pseudomonas syringae pathovar as catalysts for the synthesis of potentially prebiotic oligo- and polysaccharides
New Biotechnol.
32
597-605
2015
Priestia megaterium, Bacillus subtilis, Burkholderia cepacia, Dactylis glomerata, Erwinia amylovora, Lactobacillus gasseri, Limosilactobacillus reuteri, Fructilactobacillus sanfranciscensis, Zymomonas mobilis, Limosilactobacillus panis, Phleum pratense, Pseudomonas syringae (O68609), Gluconacetobacter diazotrophicus (Q43998), Pseudomonas syringae pv. tomato (Q883P5), Pseudomonas syringae pv. tomato (Q88BN6), Pseudomonas chlororaphis subsp. aurantiaca (Q93FU9), Bacillus licheniformis (W8GV60), Pseudomonas syringae pv. tomato DC3000 (Q883P5), Pseudomonas syringae pv. tomato DC3000 (Q88BN6)
Jakob, F.; Quintero, Y.; Musacchio, A.; Estrada-de Los Santos, P.; Hernandez, L.; Vogel, R.F.
Acetic acid bacteria encode two levansucrase types of different ecological relationship
Environ. Microbiol.
21
4151-4165
2019
Azotobacter chroococcum, Gluconobacter albidus, Pseudomonas syringae, Kozakia baliensis, Erwinia amylovora (A0A0M3KKU6), Burkholderia vietnamiensis (A4JNE0), Paraburkholderia graminis (B1G3X6), Beijerinckia indica subsp. indica (B2IF78), Zymomonas mobilis subsp. mobilis (P0DJA3), Gluconacetobacter diazotrophicus (Q43998), Beijerinckia indica subsp. indica ATCC 9039 (B2IF78), Paraburkholderia graminis ATCC 700544 (B1G3X6), Erwinia amylovora ATCC 49946 (A0A0M3KKU6), Zymomonas mobilis subsp. mobilis ATCC 31821 (P0DJA3)
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