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Literature summary for 2.4.1.10 extracted from

  • Hernandez, L.; Arrieta, J.; Menendez, C.; Vazquez, R.; Coego, A.; Suarez, V.; Selman, G.; Petit-Glatron, M.F.; Chambert, R.
    Isolation and enzymic properties of levansucrase secreted by Acetobacter diazotrophicus SRT4, a bacterium associated with sugar cane (1995), Biochem. J., 309, 113-118.
No PubMed abstract available
Search for more literature for 2.4.1.10

Inhibitors

Inhibitors Comment Organism Structure
Fe2(SO4)3
-
 Gluconacetobacter diazotrophicus
Hg2+ strong Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Gluconacetobacter diazotrophicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slightly activating Gluconacetobacter diazotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Gluconacetobacter diazotrophicus
200000
-
 above, gel filtration Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n Gluconacetobacter diazotrophicus synthesis of a high-molecular-mass fructan of the levan type glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
 i.e. Acetobacter diazotrophicus
-
Gluconacetobacter diazotrophicus
-
 constitutive LsdA, strain SRT4
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconacetobacter diazotrophicus

Reaction

Reaction Comment Organism Reaction ID
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside ping-pong mechanism Gluconacetobacter diazotrophicus
a
sucrose + [6)-beta-D-fructofuranosyl-(2->]n alpha-D-glucopyranoside = D-glucose + [6)-beta-D-fructofuranosyl-(2->]n+1 alpha-D-glucopyranoside formation of a transient fructosyl-enzyme intermediate, kinetics of the hydrolase, exchange and transfructosylation reaction Gluconacetobacter diazotrophicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n various acceptors Gluconacetobacter diazotrophicus glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
sucrose + (2,6-beta-D-fructosyl)n synthesis of a high-molecular-mass fructan of the levan type Gluconacetobacter diazotrophicus glucose + (2,6-beta-D-fructosyl)n+1
-
 ?
sucrose + D-glucose exchange reaction Gluconacetobacter diazotrophicus glucose + sucrose
-
 ?
sucrose + sucrose
-
 Gluconacetobacter diazotrophicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.5 Gluconacetobacter diazotrophicus
5
-
-
 Gluconacetobacter diazotrophicus