Information on EC 2.3.3.2 - decylcitrate synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
2.3.3.2
-
RECOMMENDED NAME
GeneOntology No.
decylcitrate synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
lauroyl-CoA + H2O + oxaloacetate = (2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
condensation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
dodecanoyl-CoA:oxaloacetate C-dodecanoyltransferase (thioester-hydrolysing, 1-carboxyundecyl-forming)
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate oxaloacetate-lyase (CoA-acylating)
-
-
-
-
2-decylcitrate synthase
-
-
-
-
EC 4.1.3.23
-
-
formerly
-
CAS REGISTRY NUMBER
COMMENTARY
9068-72-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Penicillium spiculisporum
strain Lehman
-
-
Manually annotated by BRENDA team
Penicillium spiculisporum Lehman
strain Lehman
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
11-formamidoundecanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxy-11-formamidotridecanoyl + CoA
show the reaction diagram
Penicillium spiculisporum, Penicillium spiculisporum Lehman
-
-
-
-
?
acetyl-CoA + H2O + oxaloacetate
citrate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
?
butanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxyhexane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
-
?
decanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxydodecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum, Penicillium spiculisporum Lehman
-
-
-
-
?
lauroyl-CoA + H2O + 2-oxoglutarate
?
show the reaction diagram
Penicillium spiculisporum, Penicillium spiculisporum Lehman
-
-
-
-
?
lauroyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
-
?
lauroyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum Lehman
-
-
-
-
?
lauroyl-CoA + H2O + pyruvate
?
show the reaction diagram
Penicillium spiculisporum, Penicillium spiculisporum Lehman
-
-
-
-
?
myristoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxypentadecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
-
?
octanoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxydecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum, Penicillium spiculisporum Lehman
-
-
-
-
?
palmitoyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxyheptadecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
-
?
phenylacetyl-CoA + H2O + oxaloacetate
phenylcitrate + CoA
show the reaction diagram
Penicillium spiculisporum
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
lauroyl-CoA + H2O + oxaloacetate
(2S,3S)-2-hydroxytridecane-1,2,3-tricarboxylate + CoA
show the reaction diagram
Penicillium spiculisporum, Penicillium spiculisporum Lehman
-
-
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5,5'-dithio-bis-(2-nitrobenzoic acid)
Penicillium spiculisporum
-
-
acetyl-CoA
Penicillium spiculisporum
-
-
CoASSCoA
Penicillium spiculisporum
-
-
iodoacetamide
Penicillium spiculisporum
-
-
Lauroyl-CoA
Penicillium spiculisporum
-
strong substrate inhibition, does not follow Michaelis-Menten kinetics
N-ethylmaleimide
Penicillium spiculisporum
-
-
NADH
Penicillium spiculisporum
-
at 5 mM, slight inhibition
p-chloromercuribenzoate
Penicillium spiculisporum
-
-
palmitoyl-CoA
Penicillium spiculisporum
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.03
-
11-Formamidoundecanoyl-CoA
Penicillium spiculisporum
-
-
0.0001
-
Lauroyl-CoA
Penicillium spiculisporum
-
-
0.035
-
oxaloacetate
Penicillium spiculisporum
-
at 0.021 mM lauroyl-CoA
1.2
-
oxaloacetate
Penicillium spiculisporum
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
9
-
Penicillium spiculisporum
-
above this pH
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
35
Penicillium spiculisporum
-
-
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
90000
-
Penicillium spiculisporum
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
Penicillium spiculisporum
-
2 * 45000, SDS-PAGE
dimer
Penicillium spiculisporum Lehman
-
2 * 45000, SDS-PAGE
-
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
thiols such as mercaptoethanol or cysteine stabilize
Penicillium spiculisporum
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-18°C, 0.5 M (NH4)2SO4, 20% activity loss, 2 months
Penicillium spiculisporum
-
0°C, enzyme concentration 2 mg/ml, 80 h, 50% activity
Penicillium spiculisporum
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Penicillium spiculisporum
-