Information on EC 2.3.1.166 - 2alpha-hydroxytaxane 2-O-benzoyltransferase

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The expected taxonomic range for this enzyme is: Taxus

EC NUMBER
COMMENTARY
2.3.1.166
-
RECOMMENDED NAME
GeneOntology No.
2alpha-hydroxytaxane 2-O-benzoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III = CoA + 10-deacetylbaccatin III
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acylation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
Diterpenoid biosynthesis
-
Metabolic pathways
-
taxol biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
benzoyl-CoA:taxan-2alpha-ol O-benzoyltransferase
The enzyme was studied using the semisynthetic substrate 2-debenzoyl-7,13-diacetylbaccatin III. It will not acylate the hydroxy group at 1beta, 7beta, 10beta or 13alpha of 10-deacetyl baccatin III, or at 2alpha or 5alpha of taxa-4(20),11-diene-2alpha,5alpha-diol.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2-O-benzoyltransferase
-
-
benzoyl-CoA:taxane 2alpha-O-benzoyltransferase
-
-
-
-
benzoyltransferase, taxane 2alpha-O-
-
-
-
-
mTBT
-
mutant, which partitions into the soluble (active) enzyme fraction about 5fold greater than the wild-type enzyme and therefore allows enzymatical investigations
taxane 2alpha-O-benzoyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
329318-50-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-methylbenzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl cyclohexanecarboxylate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate
CoA + ?
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III
CoA + 10-deacetylbaccatin III
show the reaction diagram
Q9FPW3, -
-
-
?
benzoyl-CoA + 2-debenzoyl-7,13-diacetylbaccatin III
CoA + 7,13-diacetylbaccatin III
show the reaction diagram
-
-
-
-
?
benzoyl-CoA + 2-debenzoyl-7,13-diacetylbaccatin III
CoA + 7,13-diacetylbaccatin III
show the reaction diagram
Q9FPW3, -
2-debenzoyl-7,13-diacetylbaccatin III is a semisynthetic substrate
-
?
benzoyl-CoA + [13-3H]-7,13-O,O-diacetyl-2-O-debenzoylbaccatin III
CoA + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
Q9FPW3, -
enzyme does not benzoylate the 1beta-, 7beta-, 10beta- or 13alpha-hydroxyl groups of 10-deacetylbaccatin III, nor does it benzoylate the 2alpha- or 5alpha-hydroxyl groups of taxa-4(20),11(12)-dien-2alpha,5alpha-diol, acetyl-CoA no cosubstrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
benzoyl-CoA + 10-deacetyl-2-debenzoylbaccatin III
CoA + 10-deacetylbaccatin III
show the reaction diagram
Q9FPW3, -
-
-
?
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.51
-
(2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate
-
reaction was performed with mutant mTBT
0.049
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate
-
reaction was performed with mutant mTBT
0.37
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate
-
reaction was performed with mutant mTBT
0.17
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate
-
reaction was performed with mutant mTBT
0.055
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate
-
reaction was performed with mutant mTBT
0.27
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate
-
reaction was performed with mutant mTBT
0.042
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate
-
reaction was performed with mutant mTBT
0.12
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate
-
reaction was performed with mutant mTBT
0.012
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate
-
reaction was performed with mutant mTBT
0.11
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate
-
reaction was performed with mutant mTBT
0.057
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate
-
reaction was performed with mutant mTBT
0.12
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate
-
reaction was performed with mutant mTBT
0.0064
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate
-
reaction was performed with mutant mTBT
0.042
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate
-
reaction was performed with mutant mTBT
0.12
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate
-
reaction was performed with mutant mTBT
0.045
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate
-
reaction was performed with mutant mTBT
0.18
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate
-
reaction was performed with mutant mTBT
0.64
-
2-debenzoyl-7,13-diacetylbaccatin III
-
-
0.3
-
benzoyl-CoA
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00417
-
(2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate
-
reaction was performed with mutant mTBT
0.02833
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate
-
reaction was performed with mutant mTBT
0.00217
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate
-
reaction was performed with mutant mTBT
0.01083
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate
-
reaction was performed with mutant mTBT
0.00038
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate
-
reaction was performed with mutant mTBT
0.00125
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate
-
reaction was performed with mutant mTBT
0.00004
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-methylbenzoate
-
-
0.00027
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate
-
reaction was performed with mutant mTBT
0.00127
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate
-
reaction was performed with mutant mTBT
0.00035
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate
-
reaction was performed with mutant mTBT
0.00317
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate
-
reaction was performed with mutant mTBT
0.02
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate
-
reaction was performed with mutant mTBT
0.000004
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl cyclohexanecarboxylate
-
reaction was performed with mutant mTBT
0.00183
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate
-
reaction was performed with mutant mTBT
0.00023
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate
-
reaction was performed with mutant mTBT
0.00017
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate
-
reaction was performed with mutant mTBT
0.00583
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate
-
reaction was performed with mutant mTBT
0.00217
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate
-
reaction was performed with mutant mTBT
0.0085
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate
-
reaction was performed with mutant mTBT
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00818
-
(2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate
-
reaction was performed with mutant mTBT
293119
0.5782
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate
-
reaction was performed with mutant mTBT
293122
0.00586
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate
-
reaction was performed with mutant mTBT
293128
0.06371
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate
-
reaction was performed with mutant mTBT
293116
0.00697
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate
-
reaction was performed with mutant mTBT
293130
0.00463
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate
-
reaction was performed with mutant mTBT
293117
0.00635
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate
-
reaction was performed with mutant mTBT
293123
0.01056
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate
-
reaction was performed with mutant mTBT
293118
0.00292
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate
-
reaction was performed with mutant mTBT
293115
0.0288
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate
-
reaction was performed with mutant mTBT
293114
0.3509
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate
-
reaction was performed with mutant mTBT
293121
0.01528
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate
-
reaction was performed with mutant mTBT
293124
0.03646
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate
-
reaction was performed with mutant mTBT
293125
0.00397
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate
-
reaction was performed with mutant mTBT
293129
0.04858
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate
-
reaction was performed with mutant mTBT
293120
0.04815
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate
-
reaction was performed with mutant mTBT
293126
0.04722
-
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate
-
reaction was performed with mutant mTBT
293127
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
9.9
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
31
-
-
enzyme assay
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.3
-
-
calculated
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
no expression in fruit and stem
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50090
-
-
calculated from sequence of cDNA
52000
-
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
ca. x * 50000, SDS-PAGE
?
-
x * 50000, SDS-PAGE
?
-
x * 50090, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed as wild type and mutant protein in Escherichia coli
-
expressed in Escherichia coli JM109
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Q19P/N23K
-
enzyme with this mutations, named mTBT, partitions into the soluble (active) enzyme fraction about 5fold greater than the wild-type enzyme
additional information
-
functional expression in Saccharomyces cerevisiae for reconstruction of taxoid biosynthesis