Cloned (Comment) | Organism |
---|---|
expressed as wild type and mutant protein in Escherichia coli | Taxus cuspidata |
Protein Variants | Comment | Organism |
---|---|---|
Q19P/N23K | enzyme with this mutations, named mTBT, partitions into the soluble (active) enzyme fraction about 5fold greater than the wild-type enzyme | Taxus cuspidata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.012 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.042 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.042 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.045 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.049 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.055 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.057 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.11 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.12 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.12 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.12 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.17 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.18 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.27 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.37 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.51 | - |
(2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate | reaction was performed with mutant mTBT | Taxus cuspidata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
SDS-PAGE | Taxus cuspidata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus cuspidata | Q9FPW3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Taxus cuspidata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-methylbenzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl cyclohexanecarboxylate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + (2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate | - |
Taxus cuspidata | CoA + ? | - |
? | |
benzoyl-CoA + [13-3H]-7,13-O,O-diacetyl-2-O-debenzoylbaccatin III | - |
Taxus cuspidata | CoA + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-O-benzoyltransferase | - |
Taxus cuspidata |
mTBT | mutant, which partitions into the soluble (active) enzyme fraction about 5fold greater than the wild-type enzyme and therefore allows enzymatical investigations | Taxus cuspidata |
TBT | - |
Taxus cuspidata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000004 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl cyclohexanecarboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00004 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-methylbenzoate | - |
Taxus cuspidata | |
0.00017 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00023 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00027 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00035 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00038 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00125 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00127 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00183 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00217 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00217 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00317 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00417 | - |
(2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00583 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.0085 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.01083 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.02 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.02833 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate | reaction was performed with mutant mTBT | Taxus cuspidata |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00292 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-methylbenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00397 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl hexanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00463 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 3-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00586 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 1,3-thiazole-5-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00635 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-chlorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00697 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-methylpropanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.00818 | - |
(2alpha,5beta,7beta,10beta,13alpha)-1-hydroxy-9-oxo-5,20-epoxytax-11-ene-2,4,7,10,13-pentayl pentaacetate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.01056 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 4-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.01528 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-2-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.0288 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl benzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.03646 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl furan-3-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.04722 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-3-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.04815 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl thiophene-2-carboxylate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.04858 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl propanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.06371 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl 2-fluorobenzoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.3509 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl butanoate | reaction was performed with mutant mTBT | Taxus cuspidata | |
0.5782 | - |
(2alpha,5beta,7beta,10beta,13alpha)-4,7,10,13-tetrakis(acetyloxy)-1-hydroxy-9-oxo-5,20-epoxytax-11-en-2-yl (2E)-but-2-enoate | reaction was performed with mutant mTBT | Taxus cuspidata |